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Importance of Cadherins Methylation in Ovarian Cancer: a Next Generation Sequencing Approach
M. Chmelarova, I. Baranova, E. Ruszova, J. Laco, K. Hrochova, E. Dvorakova, V. Palicka,
Jazyk angličtina Země Nizozemsko
Typ dokumentu časopisecké články
Grantová podpora
UHHK, 00179906
Ministerstvo Zdravotnictví Ceské Republiky
BBMRI, LM2015089
Ministerstvo Školství, Mládeže a Tělovýchovy
NLK
ProQuest Central
od 1997-03-01 do 2019-01-31
Medline Complete (EBSCOhost)
od 2014-01-01
Nursing & Allied Health Database (ProQuest)
od 1997-03-01 do 2019-01-31
Health & Medicine (ProQuest)
od 1997-03-01 do 2019-01-31
ROAD: Directory of Open Access Scholarly Resources
od 1995
- MeSH
- dospělí MeSH
- kadheriny genetika MeSH
- lidé středního věku MeSH
- lidé MeSH
- metylace DNA * MeSH
- mladý dospělý MeSH
- mucinózní adenokarcinom genetika patologie MeSH
- nádorové biomarkery genetika MeSH
- nádory endometria genetika patologie MeSH
- nádory vaječníků genetika patologie MeSH
- následné studie MeSH
- prognóza MeSH
- promotorové oblasti (genetika) MeSH
- regulace genové exprese u nádorů * MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- serózní cystadenokarcinom genetika patologie MeSH
- studie případů a kontrol MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladý dospělý MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
Epigenetic aberrations are well known to play an important role in carcinogenesis, and also have a great potential to serve as biomarkers in many types of cancers, including ovarian cancer in which sensitive and specific biomarkers and detection methods are critically needed. The aim of this study was to investigate methylation of cadherin genes CDH10, CDH13 and CDH18 in ovarian cancer tissue by comparison with control tissue. The study group consisted of 38 patients with ovarian cancer and 25 control patients. For detection of epigenetic events we used next generation sequencing, the most important data were confirmed using high-resolution melting analysis and real-time PCR. We observed significantly higher methylation in CDH13, sporadic methylation in CDH10 and loss of methylation in CDH18 in the ovarian cancer group compared with the control group. These observations suggest that changes in methylation of cadherin genes may be one of the major mechanisms associated with ovarian cancer progression. In addition, because of the high frequency of methylation of the CDH13 gene in the early stages of ovarian cancer, the analyzed CpG sites might be good targets for next study of potential ovarian cancer screening biomarkers.
Citace poskytuje Crossref.org
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- $a Chmelarova, Marcela $u Institute for Clinical Biochemistry and Diagnostics, Charles University, Faculty of Medicine and University Hospital in Hradec Kralove, Hradec Kralove, Czech Republic.
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- $a Importance of Cadherins Methylation in Ovarian Cancer: a Next Generation Sequencing Approach / $c M. Chmelarova, I. Baranova, E. Ruszova, J. Laco, K. Hrochova, E. Dvorakova, V. Palicka,
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- $a Epigenetic aberrations are well known to play an important role in carcinogenesis, and also have a great potential to serve as biomarkers in many types of cancers, including ovarian cancer in which sensitive and specific biomarkers and detection methods are critically needed. The aim of this study was to investigate methylation of cadherin genes CDH10, CDH13 and CDH18 in ovarian cancer tissue by comparison with control tissue. The study group consisted of 38 patients with ovarian cancer and 25 control patients. For detection of epigenetic events we used next generation sequencing, the most important data were confirmed using high-resolution melting analysis and real-time PCR. We observed significantly higher methylation in CDH13, sporadic methylation in CDH10 and loss of methylation in CDH18 in the ovarian cancer group compared with the control group. These observations suggest that changes in methylation of cadherin genes may be one of the major mechanisms associated with ovarian cancer progression. In addition, because of the high frequency of methylation of the CDH13 gene in the early stages of ovarian cancer, the analyzed CpG sites might be good targets for next study of potential ovarian cancer screening biomarkers.
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