-
Je něco špatně v tomto záznamu ?
Defects in memory B-cell and plasma cell subsets expressing different immunoglobulin-subclasses in patients with CVID and immunoglobulin subclass deficiencies
E. Blanco, M. Pérez-Andrés, S. Arriba-Méndez, C. Serrano, I. Criado, L. Del Pino-Molina, S. Silva, I. Madruga, M. Bakardjieva, C. Martins, A. Serra-Caetano, A. Romero, T. Contreras-Sanfeliciano, C. Bonroy, F. Sala, A. Martín, JM. Bastida, F....
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- dítě MeSH
- dospělí MeSH
- imunoglobuliny nedostatek imunologie MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- plazmatické buňky imunologie MeSH
- počet buněk MeSH
- podskupiny B-lymfocytů imunologie MeSH
- předškolní dítě MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- syndromy imunologické nedostatečnosti imunologie MeSH
- Check Tag
- dítě MeSH
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- předškolní dítě MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
BACKGROUND: Predominantly antibody deficiencies (PADs) are the most prevalent primary immunodeficiencies, but their B-cell defects and underlying genetic alterations remain largely unknown. OBJECTIVE: We investigated patients with PADs for the distribution of 41 blood B-cell and plasma cell (PC) subsets, including subsets defined by expression of distinct immunoglobulin heavy chain subclasses. METHODS: Blood samples from 139 patients with PADs, 61 patients with common variable immunodeficiency (CVID), 68 patients with selective IgA deficiency (IgAdef), 10 patients with IgG subclass deficiency with IgA deficiency, and 223 age-matched control subjects were studied by using flow cytometry with EuroFlow immunoglobulin isotype staining. Patients were classified according to their B-cell and PC immune profile, and the obtained patient clusters were correlated with clinical manifestations of PADs. RESULTS: Decreased counts of blood PCs, memory B cells (MBCs), or both expressing distinct IgA and IgG subclasses were identified in all patients with PADs. In patients with IgAdef, B-cell defects were mainly restricted to surface membrane (sm)IgA+ PCs and MBCs, with 2 clear subgroups showing strongly decreased numbers of smIgA+ PCs with mild versus severe smIgA+ MBC defects and higher frequencies of nonrespiratory tract infections, autoimmunity, and affected family members. Patients with IgG subclass deficiency with IgA deficiency and those with CVID showed defects in both smIgA+ and smIgG+ MBCs and PCs. Reduced numbers of switched PCs were systematically found in patients with CVID (absent in 98%), with 6 different defective MBC (and clinical) profiles: (1) profound decrease in MBC numbers; (2) defective CD27+ MBCs with almost normal IgG3+ MBCs; (3) absence of switched MBCs; and (4) presence of both unswitched and switched MBCs without and; (5) with IgG2+ MBCs; and (6) with IgA1+ MBCs. CONCLUSION: Distinct PAD defective B-cell patterns were identified that are associated with unique clinical profiles.
Bioinformatics service University of Salamanca Salamanca Spain
Centro de Salud Miguel Armijo Salamanca Spain
Department of Immunology Erasmus MC Rotterdam The Netherlands
Department of Laboratory Medicine University Hospital Ghent Ghent Belgium
Department of Medicine Cancer Research Centre Salamanca Spain
Hospital D Estefânia CHLC Lisbon Portugal
Instituto de Medicina Molecular Faculdade de Medicina Universidade de Lisboa Lisbon Portugal
NOVA Medical School Faculdade de Ciências Médicas Universidade Nova de Lisboa Lisbon Portugal
Servicio de Bioquímica Clínica Hospital Universitario de Salamanca Salamanca Spain
Servicio de Hematología Hospital de Navarra Pamplona Spain
Servicio de Inmunología Fundación Jiménez Díaz Madrid Spain
Servicio de Pediatría Hospital Universitario de Salamanca Salamanca Spain
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc20023702
- 003
- CZ-PrNML
- 005
- 20201214130902.0
- 007
- ta
- 008
- 201125s2019 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1016/j.jaci.2019.02.017 $2 doi
- 035 __
- $a (PubMed)30826363
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Blanco, Elena $u Department of Medicine, Cancer Research Centre (IBMCC, USAL-CSIC), Cytometry Service (NUCLEUS), University of Salamanca (USAL), Institute of Biomedical Research of Salamanca (IBSAL), Salamanca, Spain; Biomedical Research Networking Centre Consortium of Oncology (CIBERONC), number CB16/12/00400, Instituto de Salud Carlos III, Madrid, Spain.
- 245 10
- $a Defects in memory B-cell and plasma cell subsets expressing different immunoglobulin-subclasses in patients with CVID and immunoglobulin subclass deficiencies / $c E. Blanco, M. Pérez-Andrés, S. Arriba-Méndez, C. Serrano, I. Criado, L. Del Pino-Molina, S. Silva, I. Madruga, M. Bakardjieva, C. Martins, A. Serra-Caetano, A. Romero, T. Contreras-Sanfeliciano, C. Bonroy, F. Sala, A. Martín, JM. Bastida, F. Lorente, C. Prieto, I. Dávila, M. Marcos, T. Kalina, M. Vlkova, Z. Chovancova, AI. Cordeiro, J. Philippé, F. Haerynck, E. López-Granados, AE. Sousa, M. van der Burg, JJM. van Dongen, A. Orfao, EuroFlow PID group,
- 520 9_
- $a BACKGROUND: Predominantly antibody deficiencies (PADs) are the most prevalent primary immunodeficiencies, but their B-cell defects and underlying genetic alterations remain largely unknown. OBJECTIVE: We investigated patients with PADs for the distribution of 41 blood B-cell and plasma cell (PC) subsets, including subsets defined by expression of distinct immunoglobulin heavy chain subclasses. METHODS: Blood samples from 139 patients with PADs, 61 patients with common variable immunodeficiency (CVID), 68 patients with selective IgA deficiency (IgAdef), 10 patients with IgG subclass deficiency with IgA deficiency, and 223 age-matched control subjects were studied by using flow cytometry with EuroFlow immunoglobulin isotype staining. Patients were classified according to their B-cell and PC immune profile, and the obtained patient clusters were correlated with clinical manifestations of PADs. RESULTS: Decreased counts of blood PCs, memory B cells (MBCs), or both expressing distinct IgA and IgG subclasses were identified in all patients with PADs. In patients with IgAdef, B-cell defects were mainly restricted to surface membrane (sm)IgA+ PCs and MBCs, with 2 clear subgroups showing strongly decreased numbers of smIgA+ PCs with mild versus severe smIgA+ MBC defects and higher frequencies of nonrespiratory tract infections, autoimmunity, and affected family members. Patients with IgG subclass deficiency with IgA deficiency and those with CVID showed defects in both smIgA+ and smIgG+ MBCs and PCs. Reduced numbers of switched PCs were systematically found in patients with CVID (absent in 98%), with 6 different defective MBC (and clinical) profiles: (1) profound decrease in MBC numbers; (2) defective CD27+ MBCs with almost normal IgG3+ MBCs; (3) absence of switched MBCs; and (4) presence of both unswitched and switched MBCs without and; (5) with IgG2+ MBCs; and (6) with IgA1+ MBCs. CONCLUSION: Distinct PAD defective B-cell patterns were identified that are associated with unique clinical profiles.
- 650 _2
- $a mladiství $7 D000293
- 650 _2
- $a dospělí $7 D000328
- 650 _2
- $a senioři $7 D000368
- 650 _2
- $a senioři nad 80 let $7 D000369
- 650 _2
- $a podskupiny B-lymfocytů $x imunologie $7 D016175
- 650 _2
- $a počet buněk $7 D002452
- 650 _2
- $a dítě $7 D002648
- 650 _2
- $a předškolní dítě $7 D002675
- 650 _2
- $a ženské pohlaví $7 D005260
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a imunoglobuliny $x nedostatek $x imunologie $7 D007136
- 650 _2
- $a syndromy imunologické nedostatečnosti $x imunologie $7 D007153
- 650 _2
- $a mužské pohlaví $7 D008297
- 650 _2
- $a lidé středního věku $7 D008875
- 650 _2
- $a plazmatické buňky $x imunologie $7 D010950
- 650 _2
- $a mladý dospělý $7 D055815
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Pérez-Andrés, Martín $u Department of Medicine, Cancer Research Centre (IBMCC, USAL-CSIC), Cytometry Service (NUCLEUS), University of Salamanca (USAL), Institute of Biomedical Research of Salamanca (IBSAL), Salamanca, Spain; Biomedical Research Networking Centre Consortium of Oncology (CIBERONC), number CB16/12/00400, Instituto de Salud Carlos III, Madrid, Spain.
- 700 1_
- $a Arriba-Méndez, Sonia $u Servicio de Pediatría, Hospital Universitario de Salamanca, Salamanca, Spain.
- 700 1_
- $a Serrano, Cristina $u Servicio de Inmunología, Fundación Jiménez Díaz, Madrid, Spain.
- 700 1_
- $a Criado, Ignacio $u Department of Medicine, Cancer Research Centre (IBMCC, USAL-CSIC), Cytometry Service (NUCLEUS), University of Salamanca (USAL), Institute of Biomedical Research of Salamanca (IBSAL), Salamanca, Spain; Biomedical Research Networking Centre Consortium of Oncology (CIBERONC), number CB16/12/00400, Instituto de Salud Carlos III, Madrid, Spain.
- 700 1_
- $a Del Pino-Molina, Lucía $u Clinical Immunology Department, University Hospital La Paz and Physiopathology of Lymphocytes in Immunodeficiencies Group, IdiPAZ Institute for Health Research, Madrid, Spain.
- 700 1_
- $a Silva, Susana $u Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisbon, Portugal.
- 700 1_
- $a Madruga, Ignacio $u Servicio de Medicina Interna, Hospital Universitario de Salamanca, Institute for Biomedical Research of Salamanca, Department of Medicine, University of Salamanca, Salamanca, Spain.
- 700 1_
- $a Bakardjieva, Marina $u CLIP, Department of Haematology/Oncology, 2nd Faculty of Medicine, Charles University, Prague, Czech Republic.
- 700 1_
- $a Martins, Catarina $u NOVA Medical School/Faculdade de Ciências Médicas Universidade Nova de Lisboa, Lisbon, Portugal.
- 700 1_
- $a Serra-Caetano, Ana $u Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisbon, Portugal.
- 700 1_
- $a Romero, Alfonso $u Centro de Salud Miguel Armijo, Salamanca, Spain.
- 700 1_
- $a Contreras-Sanfeliciano, Teresa $u Servicio de Bioquímica Clínica, Hospital Universitario de Salamanca, Salamanca, Spain.
- 700 1_
- $a Bonroy, Carolien $u Department of Laboratory Medicine, University Hospital Ghent, Ghent, Belgium.
- 700 1_
- $a Sala, Francisco $u Servicio de Hematología, Hospital de Navarra, Pamplona, Spain.
- 700 1_
- $a Martín, Alejandro $u Servicio de Hematología, Hospital Universitario de Salamanca, Institute for Biomedical Research of Salamanca, Salamanca, Spain; Biomedical Research Networking Centre Consortium of Oncology (CIBERONC) number CB/16/12/00233, Instituto de salud Carlos III, Madrid, Spain.
- 700 1_
- $a Bastida, José María $u Servicio de Hematología, Hospital Universitario de Salamanca, Institute for Biomedical Research of Salamanca, Salamanca, Spain; Biomedical Research Networking Centre Consortium of Oncology (CIBERONC) number CB/16/12/00233, Instituto de salud Carlos III, Madrid, Spain.
- 700 1_
- $a Lorente, Félix $u Servicio de Pediatría, Hospital Universitario de Salamanca, Salamanca, Spain.
- 700 1_
- $a Prieto, Carlos $u Bioinformatics service (NUCLEUS), University of Salamanca, Salamanca, Spain.
- 700 1_
- $a Dávila, Ignacio $u Servicio de Alergia, Hospital Universitario de Salamanca, Institute for Biomedical Research of Salamanca, Biomedical and Diagnosis Science Department, University of Salamanca (USAL), Salamanca, Spain.
- 700 1_
- $a Marcos, Miguel $u Servicio de Medicina Interna, Hospital Universitario de Salamanca, Institute for Biomedical Research of Salamanca, Department of Medicine, University of Salamanca, Salamanca, Spain.
- 700 1_
- $a Kalina, Tomas $u CLIP, Department of Haematology/Oncology, 2nd Faculty of Medicine, Charles University, Prague, Czech Republic.
- 700 1_
- $a Vlkova, Marcela $u Department of Clinical Immunology and Allergology, St Anne's University Hospital, and Faculty of Medicine, Masaryk University, Brno, Czech Republic.
- 700 1_
- $a Chovancova, Zita $u Department of Clinical Immunology and Allergology, St Anne's University Hospital, and Faculty of Medicine, Masaryk University, Brno, Czech Republic.
- 700 1_
- $a Cordeiro, Ana Isabel $u Hospital D. Estefânia, CHLC, Lisbon, Portugal.
- 700 1_
- $a Philippé, Jan $u Department of Laboratory Medicine, University Hospital Ghent, Ghent, Belgium.
- 700 1_
- $a Haerynck, Filomeen $u Department of Respiratory Diseases and Department of Pediatrics and Genetics, University Hospital Ghent, Ghent, Belgium.
- 700 1_
- $a López-Granados, Eduardo $u Clinical Immunology Department, University Hospital La Paz and Physiopathology of Lymphocytes in Immunodeficiencies Group, IdiPAZ Institute for Health Research, Madrid, Spain.
- 700 1_
- $a Sousa, Ana E $u Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisbon, Portugal.
- 700 1_
- $a van der Burg, Mirjam $u Department of Immunology, Erasmus MC, Rotterdam, The Netherlands; Department of Pediatrics, Laboratory for Immunology, Leiden University Medical Center, Leiden, The Netherlands.
- 700 1_
- $a van Dongen, Jacques J M $u Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands.
- 700 1_
- $a Orfao, Alberto $u Department of Medicine, Cancer Research Centre (IBMCC, USAL-CSIC), Cytometry Service (NUCLEUS), University of Salamanca (USAL), Institute of Biomedical Research of Salamanca (IBSAL), Salamanca, Spain; Biomedical Research Networking Centre Consortium of Oncology (CIBERONC), number CB16/12/00400, Instituto de Salud Carlos III, Madrid, Spain. Electronic address: orfao@usal.es.
- 710 2_
- $a EuroFlow PID group
- 773 0_
- $w MED00002505 $t The Journal of allergy and clinical immunology $x 1097-6825 $g Roč. 144, č. 3 (2019), s. 809-824
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/30826363 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20201125 $b ABA008
- 991 __
- $a 20201214130859 $b ABA008
- 999 __
- $a ok $b bmc $g 1596021 $s 1114378
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2019 $b 144 $c 3 $d 809-824 $e 20190228 $i 1097-6825 $m Journal of allergy and clinical immunology $n J Allergy Clin Immunol $x MED00002505
- LZP __
- $a Pubmed-20201125
