• Je něco špatně v tomto záznamu ?

Hydrogen Sulfide Effects on the Survival of Lactobacilli with Emphasis on the Development of Inflammatory Bowel Diseases

I. Kushkevych, V. Kotrsová, D. Dordević, L. Buňková, M. Vítězová, A. Amedei,

. 2019 ; 9 (12) : . [pub] 20191120

Jazyk angličtina Země Švýcarsko

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc20025488

The gut microbiota is a complex component of humans that depends on diet, host genome, and lifestyle. The background: The study purpose is to find relations between nutrition, intestinal lactic acid bacteria (LAB) from various environments (human, animal intestine, and yogurt) and sulfate-reducing microbial communities in the large intestine; to compare kinetic growth parameters of LAB; and to determine their sensitivity to different concentration of hydrogen sulfide produced by intestinal sulfate-reducing bacteria. METHODS: Microbiological (isolation and identification), biochemical (electrophoresis), molecular biology methods (DNA isolation and PCR analysis), and statistical processing (average and standard error calculations) of the results were used. THE RESULTS: The toxicity of hydrogen sulfide produced by sulfate-reducing bacteria, the survival of lactic acid bacteria, and minimal inhibitory concentrations (MIC) were determined. The measured hydrogen sulfide sensitivity values were the same for L. paracasei and L. reuteri (MIC > 1.1 mM). In addition, L. plantarum and L.fermentum showed also a similar sensitivity (MIC > 0.45 mM) but significantly (p < 0.05) lower than L.reuteri and L. paracasei (1.1 > 0.45 mM). L. paracasei and L. reuteri are more sensitive to hydrogen sulfide than L. fermentum and L. plantarum. L. pentosus was sensitive to the extremely low concentration of H2S (MIC > 0.15 mM). CONCLUSIONS: The Lactobacillus species were significantly sensitive to hydrogen sulfide, which is a final metabolite of intestinal sulfate-reducing bacteria. The results are definitely helpful for a better understanding of complicated interaction among intestinal microbiota and nutrition.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc20025488
003      
CZ-PrNML
005      
20201222160232.0
007      
ta
008      
201125s2019 sz f 000 0|eng||
009      
AR
024    7_
$a 10.3390/biom9120752 $2 doi
035    __
$a (PubMed)31756903
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a sz
100    1_
$a Kushkevych, Ivan $u Department of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 753/5, 62500 Brno, Czech Republic.
245    10
$a Hydrogen Sulfide Effects on the Survival of Lactobacilli with Emphasis on the Development of Inflammatory Bowel Diseases / $c I. Kushkevych, V. Kotrsová, D. Dordević, L. Buňková, M. Vítězová, A. Amedei,
520    9_
$a The gut microbiota is a complex component of humans that depends on diet, host genome, and lifestyle. The background: The study purpose is to find relations between nutrition, intestinal lactic acid bacteria (LAB) from various environments (human, animal intestine, and yogurt) and sulfate-reducing microbial communities in the large intestine; to compare kinetic growth parameters of LAB; and to determine their sensitivity to different concentration of hydrogen sulfide produced by intestinal sulfate-reducing bacteria. METHODS: Microbiological (isolation and identification), biochemical (electrophoresis), molecular biology methods (DNA isolation and PCR analysis), and statistical processing (average and standard error calculations) of the results were used. THE RESULTS: The toxicity of hydrogen sulfide produced by sulfate-reducing bacteria, the survival of lactic acid bacteria, and minimal inhibitory concentrations (MIC) were determined. The measured hydrogen sulfide sensitivity values were the same for L. paracasei and L. reuteri (MIC > 1.1 mM). In addition, L. plantarum and L.fermentum showed also a similar sensitivity (MIC > 0.45 mM) but significantly (p < 0.05) lower than L.reuteri and L. paracasei (1.1 > 0.45 mM). L. paracasei and L. reuteri are more sensitive to hydrogen sulfide than L. fermentum and L.&nbsp;plantarum. L. pentosus was sensitive to the extremely low concentration of H2S (MIC > 0.15 mM). CONCLUSIONS: The Lactobacillus species were significantly sensitive to hydrogen sulfide, which is a final metabolite of intestinal sulfate-reducing bacteria. The results are definitely helpful for a better understanding of complicated interaction among intestinal microbiota and nutrition.
650    _2
$a zvířata $7 D000818
650    _2
$a Bacteria $x klasifikace $x izolace a purifikace $x metabolismus $7 D001419
650    _2
$a feces $x mikrobiologie $7 D005243
650    _2
$a střevní mikroflóra $x účinky léků $7 D000069196
650    _2
$a lidé $7 D006801
650    _2
$a sulfan $x metabolismus $x farmakologie $7 D006862
650    _2
$a idiopatické střevní záněty $x mikrobiologie $7 D015212
650    _2
$a střeva $x mikrobiologie $7 D007422
650    _2
$a Lactobacillus $x účinky léků $x růst a vývoj $7 D007778
650    _2
$a myši $7 D051379
650    _2
$a mikrobiální testy citlivosti $7 D008826
650    _2
$a mikrobiální viabilita $x účinky léků $7 D050296
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Kotrsová, Věra $u Department of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 753/5, 62500 Brno, Czech Republic.
700    1_
$a Dordević, Dani $u Department of Plant Origin Foodstuffs Hygiene and Technology, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, 61242 Brno, Czech Republic.
700    1_
$a Buňková, Leona $u The Department of Environmental Protection Engineering, Faculty of Technology, Tomas Bata University in Zlín, 76001 Zlín, Czech Republic.
700    1_
$a Vítězová, Monika $u Department of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 753/5, 62500 Brno, Czech Republic.
700    1_
$a Amedei, Amedeo $u Department of Experimental and Clinical Medicine, University of Florence, 50134 Florence, Italy.
773    0_
$w MED00188737 $t Biomolecules $x 2218-273X $g Roč. 9, č. 12 (2019)
856    41
$u https://pubmed.ncbi.nlm.nih.gov/31756903 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20201125 $b ABA008
991    __
$a 20201222160228 $b ABA008
999    __
$a ok $b bmc $g 1599633 $s 1116174
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2019 $b 9 $c 12 $e 20191120 $i 2218-273X $m Biomolecules $n Biomolecules $x MED00188737
LZP    __
$a Pubmed-20201125

Najít záznam

Citační ukazatele

Možnosti archivace