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Analysis of the characteristics of phosphine production by anaerobic digestion based on microbial community dynamics, metabolic pathways, and isolation of the phosphate-reducing strain
Y. Fan, X. Niu, D. Zhang, Z. Lin, M. Fu, S. Zhou,
Language English Country Great Britain
Document type Journal Article
Links
PubMed
33182078
DOI
10.1016/j.chemosphere.2020.128213
Knihovny.cz E-resources
- MeSH
- Anaerobiosis MeSH
- Bioreactors microbiology MeSH
- Clostridiales genetics metabolism MeSH
- Nitrogen metabolism MeSH
- Escherichia genetics metabolism MeSH
- Phosphates metabolism MeSH
- Phosphines analysis metabolism MeSH
- Phosphorus metabolism MeSH
- Phylogeny MeSH
- Metabolic Networks and Pathways * genetics MeSH
- Microbiota * MeSH
- Sewage microbiology MeSH
- Hydrogen metabolism MeSH
- Publication type
- Journal Article MeSH
Although phosphine is ubiquitously present in anaerobic environments, little is known regarding the microbial community dynamics and metabolic pathways associated with phosphine formation in an anaerobic digestion system. This study investigated the production of phosphine in anaerobic digestion, with results indicating that phosphine production mainly occurred during logarithmic microbial growth. Dehydrogenase and hydrogen promoted the production of phosphine, with a maximum phosphine concentration of 300 mg/m3. The abundance of Ruminococcaceae and Escherichia was observed to promote phosphine generation. The analysis of metabolic pathways based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) and the MetaCyc pathway database revealed the highest relative abundance of replication and repair in genetic information processing; further, the cofactor, prosthetic group, electron carrier, and vitamin biosynthesis were observed to be closely related to phosphine formation. A phylogenetic tree was reconstructed based on the neighbor-joining method. The results indicated the clear evolutionary position of the isolated Pseudescherichia sp. SFM4 strain, adjacent to Escherichia, with a stable phosphate-reducing ability for a maximum phosphine concentration of 26 mg/m3. The response surface experiment indicated that the initial optimal conditions for phosphine production by SFM4 could be achieved with nitrogen, carbon, and phosphorus loads of 6.17, 300, and 10 mg/L, respectively, at pH 7.47. These results provide comprehensive insights into the dynamic changes in the microbial structure, isolated single bacterial strain, and metabolic pathways associated with phosphine formation. They also provide information on the molecular biology associated with phosphorus recycling.
School of Environment and Energy South China University of Technology Guangzhou 510006 China
Sino Singapore International Joint Research Institute Guangzhou 510700 PR China
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- $a Fan, Yimin $u School of Environment and Energy, South China University of Technology, Guangzhou, 510006, China. Electronic address: 2725610181@qq.com.
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- $a Although phosphine is ubiquitously present in anaerobic environments, little is known regarding the microbial community dynamics and metabolic pathways associated with phosphine formation in an anaerobic digestion system. This study investigated the production of phosphine in anaerobic digestion, with results indicating that phosphine production mainly occurred during logarithmic microbial growth. Dehydrogenase and hydrogen promoted the production of phosphine, with a maximum phosphine concentration of 300 mg/m3. The abundance of Ruminococcaceae and Escherichia was observed to promote phosphine generation. The analysis of metabolic pathways based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) and the MetaCyc pathway database revealed the highest relative abundance of replication and repair in genetic information processing; further, the cofactor, prosthetic group, electron carrier, and vitamin biosynthesis were observed to be closely related to phosphine formation. A phylogenetic tree was reconstructed based on the neighbor-joining method. The results indicated the clear evolutionary position of the isolated Pseudescherichia sp. SFM4 strain, adjacent to Escherichia, with a stable phosphate-reducing ability for a maximum phosphine concentration of 26 mg/m3. The response surface experiment indicated that the initial optimal conditions for phosphine production by SFM4 could be achieved with nitrogen, carbon, and phosphorus loads of 6.17, 300, and 10 mg/L, respectively, at pH 7.47. These results provide comprehensive insights into the dynamic changes in the microbial structure, isolated single bacterial strain, and metabolic pathways associated with phosphine formation. They also provide information on the molecular biology associated with phosphorus recycling.
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- $a Niu, Xiaojun $u School of Environment and Energy, South China University of Technology, Guangzhou, 510006, China; School of Environmental Science and Engineering, Guangdong University of Petrochemical Technology, Maoming, 525000, China; Sino-Singapore International Joint Research Institute, Guangzhou, 510700, PR China; Guangdong Provincial Key Laboratory of Atmospheric Environment and Pollution Control, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou, 510006, PR China; The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou, 510006, PR China. Electronic address: xjniu@scut.edu.cn.
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