Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

CTX-M-producing Escherichia coli in pigs from a Czech farm during production cycle

M. Zelendova, M. Dolejska, M. Masarikova, I. Jamborova, J. Vasek, J. Smola, I. Manga, A. Cizek,

. 2020 ; 71 (4) : 369-376. [pub] 20200625

Jazyk angličtina Země Velká Británie

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc20027783

Grantová podpora
NV18-09-00605 Czech Science Foundation

We evaluated the prevalence and epidemiology of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli isolates in pigs during production cycle on a Czech farm with the history of previous use of ceftiofur. ESBL-producing E. coli isolates were obtained from rectal swabs from pigs of different age groups (suckling piglets, weaned piglets, growers and sows). Collected samples were directly cultivated on MacConkey agar with cefotaxime (2 mg l-1 ), whereas intestinal swabs of slaughtered pigs and surface swabs from pig carcasses were also pre-enriched in buffered peptone water without antimicrobials before the cultivation. Clonal relationship of selected isolates was determined by XbaI pulse-field gel electrophoresis and multi-locus sequence typing. The transferability of plasmids carrying blaCTX-M genes was tested by conjugation experiments. From all examined samples, 141 (43·7%, n = 323) were positive for ESBL-producing E. coli. All ESBL-producing isolates showed resistance to multiple antimicrobials and were positive for blaCTX-M genes. The blaCTX-M-1 was carried by conjugative IncN/ST1 plasmids (c. 40-45 kb) while the blaCTX-M-15 was located on conjugative F plasmids with F:18:A5:B1 formula (c. 165 kb). This study demonstrated the persistence of CTX-M-positive E. coli isolates 2 months after banner of ceftiofur usage and indicated possible risk of transmission of these isolates to humans via the food chain.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc20027783
003      
CZ-PrNML
005      
20210114152343.0
007      
ta
008      
210105s2020 xxk f 000 0|eng||
009      
AR
024    7_
$a 10.1111/lam.13331 $2 doi
035    __
$a (PubMed)32452042
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxk
100    1_
$a Zelendova, M $u CEITEC VFU, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic. Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic.
245    10
$a CTX-M-producing Escherichia coli in pigs from a Czech farm during production cycle / $c M. Zelendova, M. Dolejska, M. Masarikova, I. Jamborova, J. Vasek, J. Smola, I. Manga, A. Cizek,
520    9_
$a We evaluated the prevalence and epidemiology of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli isolates in pigs during production cycle on a Czech farm with the history of previous use of ceftiofur. ESBL-producing E. coli isolates were obtained from rectal swabs from pigs of different age groups (suckling piglets, weaned piglets, growers and sows). Collected samples were directly cultivated on MacConkey agar with cefotaxime (2 mg l-1 ), whereas intestinal swabs of slaughtered pigs and surface swabs from pig carcasses were also pre-enriched in buffered peptone water without antimicrobials before the cultivation. Clonal relationship of selected isolates was determined by XbaI pulse-field gel electrophoresis and multi-locus sequence typing. The transferability of plasmids carrying blaCTX-M genes was tested by conjugation experiments. From all examined samples, 141 (43·7%, n = 323) were positive for ESBL-producing E. coli. All ESBL-producing isolates showed resistance to multiple antimicrobials and were positive for blaCTX-M genes. The blaCTX-M-1 was carried by conjugative IncN/ST1 plasmids (c. 40-45 kb) while the blaCTX-M-15 was located on conjugative F plasmids with F:18:A5:B1 formula (c. 165 kb). This study demonstrated the persistence of CTX-M-positive E. coli isolates 2 months after banner of ceftiofur usage and indicated possible risk of transmission of these isolates to humans via the food chain.
650    _2
$a zvířata $7 D000818
650    _2
$a Escherichia coli $x enzymologie $x genetika $x izolace a purifikace $7 D004926
650    _2
$a infekce vyvolané Escherichia coli $x mikrobiologie $x patofyziologie $x veterinární $7 D004927
650    _2
$a proteiny z Escherichia coli $x genetika $x metabolismus $7 D029968
650    _2
$a farmy $7 D000072480
650    _2
$a lidé $7 D006801
650    _2
$a multilokusová sekvenční typizace $7 D058885
650    _2
$a plazmidy $x genetika $x metabolismus $7 D010957
650    _2
$a prasata $x růst a vývoj $x mikrobiologie $7 D013552
650    _2
$a nemoci prasat $x mikrobiologie $x patofyziologie $7 D013553
650    _2
$a beta-laktamasy $x genetika $x metabolismus $7 D001618
651    _2
$a Česká republika $7 D018153
655    _2
$a časopisecké články $7 D016428
700    1_
$a Dolejska, M $u CEITEC VFU, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic. Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic.
700    1_
$a Masarikova, M $u CEITEC VFU, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic. Department of Infectious Diseases and Microbiology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic.
700    1_
$a Jamborova, I $u CEITEC VFU, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic.
700    1_
$a Vasek, J $u Ruminant and Swine Clinic, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic.
700    1_
$a Smola, J $u CEITEC VFU, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic. Ruminant and Swine Clinic, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic.
700    1_
$a Manga, I $u CEITEC VFU, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic. Department of Infectious Diseases and Microbiology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic.
700    1_
$a Cizek, A $u CEITEC VFU, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic. Department of Infectious Diseases and Microbiology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic.
773    0_
$w MED00003137 $t Letters in applied microbiology $x 1472-765X $g Roč. 71, č. 4 (2020), s. 369-376
856    41
$u https://pubmed.ncbi.nlm.nih.gov/32452042 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20210105 $b ABA008
991    __
$a 20210114152341 $b ABA008
999    __
$a ok $b bmc $g 1608118 $s 1118963
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2020 $b 71 $c 4 $d 369-376 $e 20200625 $i 1472-765X $m Letters in applied microbiology $n Lett Appl Microbiol $x MED00003137
GRA    __
$a NV18-09-00605 $p Czech Science Foundation
LZP    __
$a Pubmed-20210105

Najít záznam

Citační ukazatele

Možnosti archivace