• Something wrong with this record ?

A novel accurate LC-MS/MS method for quantitative determination of Z-lumirubin

J. Jašprová, A. Dvořák, M. Vecka, M. Leníček, O. Lacina, P. Valášková, M. Zapadlo, R. Plavka, P. Klán, L. Vítek,

. 2020 ; 10 (1) : 4411. [pub] 20200310

Language English Country Great Britain

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc20028391

Although phototherapy (PT) is a standard treatment for neonatal jaundice, no validated clinical methods for determination of bilirubin phototherapy products are available. Thus, the aim of our study was to establish a such method for clinical use. To achieve this aim, a LC-MS/MS assay for simultaneous determination of Z-lumirubin (LR) and unconjugated bilirubin (UCB) was conducted. LR was purified after irradiation of UCB at 460 nm. The assay was tested on human sera from PT-treated neonates. Samples were separated on a HPLC system with a triple quadrupole mass spectrometer detector. The instrument response was linear up to 5.8 and 23.4 mg/dL for LR and UCB, respectively, with submicromolar limits of detection and validity parameters relevant for use in clinical medicine. Exposure of newborns to PT raised serum LR concentrations three-fold (p < 0.01), but the absolute concentrations were low (0.37 ± 0.16 mg/dL), despite a dramatic decrease of serum UCB concentrations (13.6 ± 2.2 vs. 10.3 ± 3.3 mg/dL, p < 0.01). A LC-MS/MS method for the simultaneous determination of LR and UCB in human serum was established and validated for clinical use. This method should help to monitor neonates on PT, as well as to improve our understanding of both the kinetics and biology of bilirubin phototherapy products.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc20028391
003      
CZ-PrNML
005      
20210114153725.0
007      
ta
008      
210105s2020 xxk f 000 0|eng||
009      
AR
024    7_
$a 10.1038/s41598-020-61280-z $2 doi
035    __
$a (PubMed)32157102
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxk
100    1_
$a Jašprová, Jana $u Institute of Medical Biochemistry and Laboratory Diagnostics, Faculty General Hospital and 1st Faculty of Medicine, Charles University, Prague, Czech Republic.
245    12
$a A novel accurate LC-MS/MS method for quantitative determination of Z-lumirubin / $c J. Jašprová, A. Dvořák, M. Vecka, M. Leníček, O. Lacina, P. Valášková, M. Zapadlo, R. Plavka, P. Klán, L. Vítek,
520    9_
$a Although phototherapy (PT) is a standard treatment for neonatal jaundice, no validated clinical methods for determination of bilirubin phototherapy products are available. Thus, the aim of our study was to establish a such method for clinical use. To achieve this aim, a LC-MS/MS assay for simultaneous determination of Z-lumirubin (LR) and unconjugated bilirubin (UCB) was conducted. LR was purified after irradiation of UCB at 460 nm. The assay was tested on human sera from PT-treated neonates. Samples were separated on a HPLC system with a triple quadrupole mass spectrometer detector. The instrument response was linear up to 5.8 and 23.4 mg/dL for LR and UCB, respectively, with submicromolar limits of detection and validity parameters relevant for use in clinical medicine. Exposure of newborns to PT raised serum LR concentrations three-fold (p < 0.01), but the absolute concentrations were low (0.37 ± 0.16 mg/dL), despite a dramatic decrease of serum UCB concentrations (13.6 ± 2.2 vs. 10.3 ± 3.3 mg/dL, p < 0.01). A LC-MS/MS method for the simultaneous determination of LR and UCB in human serum was established and validated for clinical use. This method should help to monitor neonates on PT, as well as to improve our understanding of both the kinetics and biology of bilirubin phototherapy products.
650    _2
$a bilirubin $x analogy a deriváty $x krev $x chemie $7 D001663
650    _2
$a chromatografie kapalinová $7 D002853
650    _2
$a lidé $7 D006801
650    _2
$a novorozenec $7 D007231
650    _2
$a novorozenecká žloutenka $x krev $x terapie $7 D007567
650    _2
$a molekulární struktura $7 D015394
650    _2
$a fototerapie $x metody $7 D010789
650    _2
$a sérum $x chemie $7 D044967
650    _2
$a tandemová hmotnostní spektrometrie $7 D053719
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Dvořák, Aleš $u Institute of Medical Biochemistry and Laboratory Diagnostics, Faculty General Hospital and 1st Faculty of Medicine, Charles University, Prague, Czech Republic.
700    1_
$a Vecka, Marek $u Institute of Medical Biochemistry and Laboratory Diagnostics, Faculty General Hospital and 1st Faculty of Medicine, Charles University, Prague, Czech Republic.
700    1_
$a Leníček, Martin $u Institute of Medical Biochemistry and Laboratory Diagnostics, Faculty General Hospital and 1st Faculty of Medicine, Charles University, Prague, Czech Republic.
700    1_
$a Lacina, Ondřej $u HPST, s.r.o., Prague, Czech Republic.
700    1_
$a Valášková, Petra $u Institute of Medical Biochemistry and Laboratory Diagnostics, Faculty General Hospital and 1st Faculty of Medicine, Charles University, Prague, Czech Republic.
700    1_
$a Zapadlo, Miloš $u Department of Pediatrics and Neonatology, Faculty General Hospital and 1st Faculty of Medicine, Charles University, Prague, Czech Republic.
700    1_
$a Plavka, Richard $u Department of Pediatrics and Neonatology, Faculty General Hospital and 1st Faculty of Medicine, Charles University, Prague, Czech Republic.
700    1_
$a Klán, Petr $u Department of Chemistry and Recetox, Faculty of Science, Masaryk University, Brno, Czech Republic.
700    1_
$a Vítek, Libor $u Institute of Medical Biochemistry and Laboratory Diagnostics, Faculty General Hospital and 1st Faculty of Medicine, Charles University, Prague, Czech Republic. vitek@cesnet.cz. 4th Department of Internal Medicine, Faculty General Hospital and 1st Faculty of Medicine, Charles University, Prague, Czech Republic. vitek@cesnet.cz.
773    0_
$w MED00182195 $t Scientific reports $x 2045-2322 $g Roč. 10, č. 1 (2020), s. 4411
856    41
$u https://pubmed.ncbi.nlm.nih.gov/32157102 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20210105 $b ABA008
991    __
$a 20210114153722 $b ABA008
999    __
$a ok $b bmc $g 1608726 $s 1119571
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2020 $b 10 $c 1 $d 4411 $e 20200310 $i 2045-2322 $m Scientific reports $n Sci Rep $x MED00182195
LZP    __
$a Pubmed-20210105

Find record

Citation metrics

Archiving options