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pDNA conjugated with citrate capped silver nanoparticles towards ultrasensitive bio-assay of haemophilus influenza in human biofluids: A novel optical biosensor
S. Hassanpour, A. Saadati, M. Hasanzadeh,
Language English Country Great Britain
Document type Journal Article
- MeSH
- Biosensing Techniques instrumentation methods MeSH
- Biological Assay MeSH
- DNA, Bacterial analysis genetics MeSH
- DNA Probes chemistry genetics MeSH
- Haemophilus influenzae genetics isolation & purification MeSH
- Nucleic Acid Hybridization MeSH
- Metal Nanoparticles chemistry MeSH
- Citric Acid chemistry MeSH
- Humans MeSH
- Limit of Detection MeSH
- Sensitivity and Specificity MeSH
- Silver chemistry MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
A sensitive and specific approach was developed for the determination of Haemophilus influenza using DNA based bio-assay. In this study, citrate capped silver nanoparticle was synthesized and employed for bioconjugation with pDNA toward target sequences detection. In this study, synthesized probe (SH-5'-AAT TTT CCA ACT TTT TCA CCT GCA T-3') of Haemophilus influenza was detected with great sensitivity and selectivity after hybridization with cDNA (5'-ATG CAG GTG AAA AAG TTG GAA AAT T-3'). Regarding to the obtained results, the low limit of quantification (LLOQ) of DNA sample was 1 ZM using 15 μL of probe and 200 μL of Cit/AgNPs. According to ultra-sensitivity of the fabricated optical DNA-based bio-assay, it has potential for bacterial determination both in clinical and environmental specimens. To evaluate the selectivity of developed DNA based biosensor, three mismatch sequences were applied. Finally, the designed genosensor is a significant diagnostic strategy for detection of Haemophilus influenza with great selectivity.
Biotechnology Research Center Tabriz University of Medical Sciences Tabriz Iran
Nutrition Research Center Tabriz University of Medical Sciences Tabriz Iran
Pharmaceutical Analysis Research Center Tabriz University of Medical Sciences Tabriz Iran
References provided by Crossref.org
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- $a A sensitive and specific approach was developed for the determination of Haemophilus influenza using DNA based bio-assay. In this study, citrate capped silver nanoparticle was synthesized and employed for bioconjugation with pDNA toward target sequences detection. In this study, synthesized probe (SH-5'-AAT TTT CCA ACT TTT TCA CCT GCA T-3') of Haemophilus influenza was detected with great sensitivity and selectivity after hybridization with cDNA (5'-ATG CAG GTG AAA AAG TTG GAA AAT T-3'). Regarding to the obtained results, the low limit of quantification (LLOQ) of DNA sample was 1 ZM using 15 μL of probe and 200 μL of Cit/AgNPs. According to ultra-sensitivity of the fabricated optical DNA-based bio-assay, it has potential for bacterial determination both in clinical and environmental specimens. To evaluate the selectivity of developed DNA based biosensor, three mismatch sequences were applied. Finally, the designed genosensor is a significant diagnostic strategy for detection of Haemophilus influenza with great selectivity.
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