CD molecules are surface molecules expressed on cells of the immune system that play key roles in immune cell-cell communication and sensing the microenvironment. These molecules are essential markers for the identification and isolation of leukocytes and lymphocyte subsets. Here, we present the results of the first phase of the CD Maps study, mapping the expression of CD1-CD100 (n = 110) on 47 immune cell subsets from blood, thymus, and tonsil using an eight-color standardized EuroFlow approach and quantification of expression. The resulting dataset included median antibody binding capacities (ABCs) and percentage of positivity for all markers on all subsets and was developed into an interactive CD Maps web resource. Using the resource, we examined differentially expressed proteins between granulocyte, monocyte, and dendritic cell subsets, and profiled dynamic expression of markers during thymocyte differentiation, T-cell maturation, and between functionally distinct B-cell subset clusters. The CD Maps resource will serve as a benchmark of antibody reactivities ensuring improved reproducibility of flow cytometry-based research. Moreover, it will provide a full picture of the surfaceome of human immune cells and serves as a useful platform to increase our understanding of leukocyte biology, as well as to facilitate the identification of new biomarkers and therapeutic targets of immunological and hematological diseases.

CLIP Childhood Leukaemia Investigation Prague Department of Paediatric Haematology and Oncology Charles University Prague Czechia

CLIP Childhood Leukaemia Investigation Prague Department of Paediatric Haematology and Oncology Charles University Prague Czechia Department of Paediatric Haematology and Oncology University Hospital Motol Prague Czechia

Department of Biomedical Sciences University of Barcelona Barcelona Spain

Department of Immunology Erasmus MC University Medical Center Rotterdam Netherlands

Department of Immunology Erasmus MC University Medical Center Rotterdam Netherlands Department of Immunology and Pathology Monash University and the Alfred Hospital Melbourne VIC Australia

Department of Medicine Cancer Research Centre Instituto de Salud Carlos 3 Madrid Spain

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$a CD Maps-Dynamic Profiling of CD1-CD100 Surface Expression on Human Leukocyte and Lymphocyte Subsets / $c T. Kalina, K. Fišer, M. Pérez-Andrés, D. Kuzílková, M. Cuenca, SJW. Bartol, E. Blanco, P. Engel, MC. van Zelm,

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