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Generation of Remosomes by the SWI/SNF Chromatin Remodeler Family
MS. Shukla, SH. Syed, R. Boopathi, EB. Simon, S. Nahata, L. Ramos, D. Dalkara, C. Moskalenko, A. Travers, D. Angelov, S. Dimitrov, A. Hamiche, J. Bednar,
Language English Country Great Britain
Document type Journal Article, Research Support, Non-U.S. Gov't
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- MeSH
- Adenosine Triphosphate metabolism pharmacology MeSH
- Cell-Free System MeSH
- Chromosomal Proteins, Non-Histone physiology MeSH
- DNA, Bacterial metabolism MeSH
- DNA Footprinting MeSH
- Fungal Proteins physiology MeSH
- Histones genetics metabolism MeSH
- Microscopy, Atomic Force MeSH
- Multiprotein Complexes physiology MeSH
- Nucleosomes physiology ultrastructure MeSH
- Plasmids chemistry MeSH
- RNA-Binding Proteins physiology MeSH
- Recombinant Proteins metabolism MeSH
- Deoxyribonucleases, Type II Site-Specific MeSH
- Chromatin Assembly and Disassembly genetics physiology MeSH
- Saccharomyces cerevisiae metabolism ultrastructure MeSH
- Xenopus laevis genetics MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Chromatin remodelers are complexes able to both alter histone-DNA interactions and to mobilize nucleosomes. The mechanism of their action and the conformation of remodeled nucleosomes remain a matter of debates. In this work we compared the type and structure of the products of nucleosome remodeling by SWI/SNF and ACF complexes using high-resolution microscopy combined with novel biochemical approaches. We find that SWI/SNF generates a multitude of nucleosome-like metastable particles termed "remosomes". Restriction enzyme accessibility assay, DNase I footprinting and AFM experiments reveal perturbed histone-DNA interactions within these particles. Electron cryo-microscopy shows that remosomes adopt a variety of different structures with variable irregular DNA path, similar to those described upon RSC remodeling. Remosome DNA accessibility to restriction enzymes is also markedly increased. We suggest that the generation of remosomes is a common feature of the SWI/SNF family remodelers. In contrast, the ACF remodeler, belonging to ISWI family, only produces repositioned nucleosomes and no evidence for particles associated with extra DNA, or perturbed DNA paths was found. The remosome generation by the SWI/SNF type of remodelers may represent a novel mechanism involved in processes where nucleosomal DNA accessibility is required, such as DNA repair or transcription regulation.
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- $a Shukla, Manu Shubhdarshan $u Université de Lyon, Laboratoire de Biologie et Modélisation de la Cellule, CNRS-UMR 5239, Ecole Normale Supérieure de Lyon, 46 Allée d'Italie, 69364, Lyon, cedex 07, France. v1mshukl@exseed.ed.ac.uk. Université Grenoble Alpes, CNRS UMR 5309, INSERM U1209, Institute for Advanced Biosciences (IAB), Site Santé - Allée des Alpes, 38700, La Tronche, France. v1mshukl@exseed.ed.ac.uk. Wellcome Centre for Cell Biology and Institute of Cell Biology, School of Biological Sciences, The University of Edinburgh, Swann Building, King's Buildings, Mayfield Road, Edinburgh, EH9 3BF, United Kingdom. v1mshukl@exseed.ed.ac.uk.
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- $a Generation of Remosomes by the SWI/SNF Chromatin Remodeler Family / $c MS. Shukla, SH. Syed, R. Boopathi, EB. Simon, S. Nahata, L. Ramos, D. Dalkara, C. Moskalenko, A. Travers, D. Angelov, S. Dimitrov, A. Hamiche, J. Bednar,
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