-
Je něco špatně v tomto záznamu ?
Reactive Oxygen Species Imaging in U937 Cells
A. Prasad, M. Sedlářová, A. Balukova, A. Ovsii, M. Rác, M. Křupka, S. Kasai, P. Pospíšil,
Jazyk angličtina Země Švýcarsko
Typ dokumentu časopisecké články
Free Medical Journals od 2010
PubMed Central od 2010
Europe PubMed Central od 2010
Open Access Digital Library od 2010-01-01
Open Access Digital Library od 2010-01-01
ROAD: Directory of Open Access Scholarly Resources od 2010
Odkazy
PubMed
33178031
DOI
10.3389/fphys.2020.552569
Knihovny.cz E-zdroje
- Publikační typ
- časopisecké články MeSH
The U937 cell culture is a pro-monocytic, human histiocytic lymphoma cell line. These monocytes can differentiate into either macrophages or dendritic cells (antigen-presenting cells) depending on the initiators. The U937 cells activated in the presence of phorbol 12-myristate 13-acetate (PMA) change their morphology into macrophage-like cells creating pseudopodia and adhering generously. Macrophages are known to produce reactive oxygen species (ROS) mostly during phagocytosis of foreign particles, an important non-specific immune response. Recently, we have focused on the role of hydroxyl radical (HO∙) and provide evidence on its importance for differentiation in U937 cells. Based on electron paramagnetic resonance (EPR) spectroscopy combined with confocal laser scanning microscopy (CLSM), formation of HO∙ was confirmed within the cells undergoing differentiation and/or apoptosis during the PMA treatment. This study aims to increase our knowledge of ROS metabolism in model cell lines used in human research.
Department of Botany Faculty of Science Palacký University Olomouc Czechia
Department of Immunology Faculty of Medicine and Dentistry Palacký University Olomouc Czechia
Graduate Department of Electronics Tohoku Institute of Technology Sendai Japan
- 000
- 00000naa a2200000 a 4500
- 001
- bmc21001965
- 003
- CZ-PrNML
- 005
- 20210126092715.0
- 007
- ta
- 008
- 210105s2020 sz f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.3389/fphys.2020.552569 $2 doi
- 035 __
- $a (PubMed)33178031
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a sz
- 100 1_
- $a Prasad, Ankush $u Department of Biophysics, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Olomouc, Czechia.
- 245 10
- $a Reactive Oxygen Species Imaging in U937 Cells. / $c A. Prasad, M. Sedlářová, A. Balukova, A. Ovsii, M. Rác, M. Křupka, S. Kasai, P. Pospíšil,
- 520 9_
- $a The U937 cell culture is a pro-monocytic, human histiocytic lymphoma cell line. These monocytes can differentiate into either macrophages or dendritic cells (antigen-presenting cells) depending on the initiators. The U937 cells activated in the presence of phorbol 12-myristate 13-acetate (PMA) change their morphology into macrophage-like cells creating pseudopodia and adhering generously. Macrophages are known to produce reactive oxygen species (ROS) mostly during phagocytosis of foreign particles, an important non-specific immune response. Recently, we have focused on the role of hydroxyl radical (HO∙) and provide evidence on its importance for differentiation in U937 cells. Based on electron paramagnetic resonance (EPR) spectroscopy combined with confocal laser scanning microscopy (CLSM), formation of HO∙ was confirmed within the cells undergoing differentiation and/or apoptosis during the PMA treatment. This study aims to increase our knowledge of ROS metabolism in model cell lines used in human research.
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Sedlářová, Michaela $u Department of Botany, Faculty of Science, Palacký University, Olomouc, Czechia.
- 700 1_
- $a Balukova, Anastasiia $u Department of Biophysics, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Olomouc, Czechia.
- 700 1_
- $a Ovsii, Alina $u Department of Biophysics, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Olomouc, Czechia.
- 700 1_
- $a Rác, Marek $u Department of Biophysics, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Olomouc, Czechia.
- 700 1_
- $a Křupka, Michal $u Department of Immunology, Faculty of Medicine and Dentistry, Palacký University, Olomouc, Czechia.
- 700 1_
- $a Kasai, Shigenobu $u Graduate Department of Electronics, Tohoku Institute of Technology, Sendai, Japan.
- 700 1_
- $a Pospíšil, Pavel $u Department of Biophysics, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Olomouc, Czechia.
- 773 0_
- $w MED00174601 $t Frontiers in physiology $x 1664-042X $g Roč. 11, č. - (2020), s. 552569
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/33178031 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y - $z 0
- 990 __
- $a 20210105 $b ABA008
- 991 __
- $a 20210126092711 $b ABA008
- 999 __
- $a ind $b bmc $g 1614094 $s 1122249
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2020 $b 11 $c - $d 552569 $e 20201015 $i 1664-042X $m Frontiers in physiology $n Front. physiol. $x MED00174601
- LZP __
- $a Pubmed-20210105