• Something wrong with this record ?

Assessment of antifungal resistance and associated molecular mechanism in Candida albicans isolates from different cohorts of patients in North Indian state of Haryana

A. Kumar, R. Nair, M. Kumar, A. Banerjee, A. Chakrabarti, SM. Rudramurthy, R. Bagga, NA. Gaur, AK. Mondal, R. Prasad

. 2020 ; 65 (4) : 747-754. [pub] 20200326

Language English Country United States

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc21011239

The present study examines the trend in distribution of Candida species and their antifungal resistance patterns in hospitals across Haryana, a North Indian state with poorly addressed epidemiology of fungal infections. In our collection of 228 Candida isolates, Candida albicans dominated in both high vaginal swab (HVS) and urine samples while Candida glabrata and Candida tropicalis were the second-highest non-albicans Candida species (NAC), respectively. Of note, in blood samples, C. tropicalis and C. albicans were present in equal numbers. All 228 isolates were subjected to antifungal susceptibility tests, whereby 51% of C. albicans recovered from HVS samples displayed fluconazole resistance. To understand its mechanistic basis, expression profiling of efflux pump genes CDR1, CDR2, MDR1 and azole drug target, ERG11 was performed in 20 randomly selected resistant isolates, wherein many isolates elicited higher expression. Further, ERG11 gene sequencing suggested that most of the isolates harbored mutations, which are not reported with azole resistance. However, one isolate, RPCA9 (MIC 64 μg/mL) harbored triple mutation (Y132C, F145L, A114V), wherein Y132 and F145 sites were previously implicated in azole resistance. Interestingly, one isolate, (RPCA61) having MIC > 128 μg/mL harbored a novel mutation, G129R. Of note, HVS isolates RPCA 21, RPCA 22, and RPCA 44 (MICs 64 to > 128 μg/mL) did not show any change in alteration in ERG11 or overexpression of efflux pump genes. Together, this study presents a first report of Candida infections in selected hospitals of Haryana State.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc21011239
003      
CZ-PrNML
005      
20210420110147.0
007      
ta
008      
210420s2020 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1007/s12223-020-00785-6 $2 doi
035    __
$a (PubMed)32219719
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Kumar, Ashok $u Amity Institute of Biotechnology and Amity Institute of Integrative Sciences and Health, Amity University Haryana, Amity Education Valley, Gurugram, 122413, India
245    10
$a Assessment of antifungal resistance and associated molecular mechanism in Candida albicans isolates from different cohorts of patients in North Indian state of Haryana / $c A. Kumar, R. Nair, M. Kumar, A. Banerjee, A. Chakrabarti, SM. Rudramurthy, R. Bagga, NA. Gaur, AK. Mondal, R. Prasad
520    9_
$a The present study examines the trend in distribution of Candida species and their antifungal resistance patterns in hospitals across Haryana, a North Indian state with poorly addressed epidemiology of fungal infections. In our collection of 228 Candida isolates, Candida albicans dominated in both high vaginal swab (HVS) and urine samples while Candida glabrata and Candida tropicalis were the second-highest non-albicans Candida species (NAC), respectively. Of note, in blood samples, C. tropicalis and C. albicans were present in equal numbers. All 228 isolates were subjected to antifungal susceptibility tests, whereby 51% of C. albicans recovered from HVS samples displayed fluconazole resistance. To understand its mechanistic basis, expression profiling of efflux pump genes CDR1, CDR2, MDR1 and azole drug target, ERG11 was performed in 20 randomly selected resistant isolates, wherein many isolates elicited higher expression. Further, ERG11 gene sequencing suggested that most of the isolates harbored mutations, which are not reported with azole resistance. However, one isolate, RPCA9 (MIC 64 μg/mL) harbored triple mutation (Y132C, F145L, A114V), wherein Y132 and F145 sites were previously implicated in azole resistance. Interestingly, one isolate, (RPCA61) having MIC > 128 μg/mL harbored a novel mutation, G129R. Of note, HVS isolates RPCA 21, RPCA 22, and RPCA 44 (MICs 64 to > 128 μg/mL) did not show any change in alteration in ERG11 or overexpression of efflux pump genes. Together, this study presents a first report of Candida infections in selected hospitals of Haryana State.
650    _2
$a antifungální látky $x farmakologie $7 D000935
650    _2
$a azoly $x farmakologie $7 D001393
650    _2
$a Candida $x klasifikace $x účinky léků $x genetika $x izolace a purifikace $7 D002175
650    _2
$a Candida albicans $x účinky léků $x genetika $x izolace a purifikace $7 D002176
650    _2
$a kandidóza $x epidemiologie $x mikrobiologie $7 D002177
650    _2
$a systém (enzymů) cytochromů P-450 $x genetika $7 D003577
650    _2
$a fungální léková rezistence $x genetika $7 D025141
650    _2
$a fungální proteiny $x genetika $7 D005656
650    _2
$a regulace genové exprese u hub $7 D015966
650    _2
$a geny MDR $x genetika $7 D019450
650    _2
$a nemocnice $7 D006761
650    _2
$a lidé $7 D006801
650    _2
$a mikrobiální testy citlivosti $7 D008826
650    _2
$a mutace $7 D009154
650    _2
$a retrospektivní studie $7 D012189
651    _2
$a Indie $x epidemiologie $7 D007194
655    _2
$a časopisecké články $7 D016428
700    1_
$a Nair, Remya $u Amity Institute of Biotechnology and Amity Institute of Integrative Sciences and Health, Amity University Haryana, Amity Education Valley, Gurugram, 122413, India
700    1_
$a Kumar, Mohit $u Amity Institute of Biotechnology and Amity Institute of Integrative Sciences and Health, Amity University Haryana, Amity Education Valley, Gurugram, 122413, India $u International Centre for Genetic Engineering and Biotechnology, New Delhi, 110067, India
700    1_
$a Banerjee, Atanu $u Amity Institute of Biotechnology and Amity Institute of Integrative Sciences and Health, Amity University Haryana, Amity Education Valley, Gurugram, 122413, India
700    1_
$a Chakrabarti, Arunaloke $u The Postgraduate Institute of Medical Education and Research (PGIMER), Chandigarh, India
700    1_
$a Rudramurthy, Shivaprakash M $u The Postgraduate Institute of Medical Education and Research (PGIMER), Chandigarh, India
700    1_
$a Bagga, Ruchika $u Fortis Memorial Research Institute (FMRI), Gurugram, India
700    1_
$a Gaur, Naseem A $u International Centre for Genetic Engineering and Biotechnology, New Delhi, 110067, India
700    1_
$a Mondal, Alok K $u School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067, India
700    1_
$a Prasad, Rajendra $u Amity Institute of Biotechnology and Amity Institute of Integrative Sciences and Health, Amity University Haryana, Amity Education Valley, Gurugram, 122413, India. rprasad@ggn.amity.edu
773    0_
$w MED00011005 $t Folia microbiologica $x 1874-9356 $g Roč. 65, č. 4 (2020), s. 747-754
856    41
$u https://pubmed.ncbi.nlm.nih.gov/32219719 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20210420 $b ABA008
991    __
$a 20210420110143 $b ABA008
999    __
$a ok $b bmc $g 1643904 $s 1131618
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2020 $b 65 $c 4 $d 747-754 $e 20200326 $i 1874-9356 $m Folia microbiologica $n Folia microbiol. (Prague) $x MED00011005
LZP    __
$a Pubmed-20210420
Back

Find record

Citation metrics

Archiving options