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Insufficient repeatability and reproducibility of MALDI-TOF MS-based identification of MRSA

V. Paskova, K. Chudejova, A. Sramkova, L. Kraftova, V. Jakubu, EA. Petinaki, H. Zemlickova, K. Neradova, CC. Papagiannitsis, J. Hrabak

. 2020 ; 65 (5) : 895-900. [pub] 20200702

Language English Country United States

Document type Journal Article, Multicenter Study

Persistent link   https://www.medvik.cz/link/bmc21011252

Grant support
NV19-05-00541 Ministerstvo Zdravotnictví Ceské Republiky

Rapid identification of methicillin-resistant Staphylococcus aureus (MRSA) is essential for proper initial antibiotic therapy and timely set up of hygienic measures. Recently, detection of MRSA using MALDI-TOF mass spectrometer mediated by the peptide-phenol-soluble modulin (PSM-mec)-linked to the class A mec gene complex present in SCCmec cassettes types II, III, and VIII of MRSA strains, has been commercially available. We present here a multicentre study on MALDI-TOF MS detection of MRSA evincing a poor repeatability and reproducibility of the assay. The sensitivity of the assay varies between 50 and 90% in strains carrying psmMEC and psmδ genes encoding for PSM-mec and δ-toxin (a member of the PSM peptide family), respectively. No false positive results were found. The very major error calculation was 30% and the major error achieved 0%. Interlaboratory repeatability varies between 0 and 100%. No significant difference was observed with the use of different cultivation media. Our data showed a poor sensitivity of the method excluding it from the use in routine laboratory testing.

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$a Paskova, Veronika $u Department of Microbiology, Faculty of Medicine and University Hospital in Pilsen, Charles University, Pilsen, Czech Republic ; Biomedical Center, Faculty of Medicine, Charles University, Pilsen, Czech Republic
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$a Insufficient repeatability and reproducibility of MALDI-TOF MS-based identification of MRSA / $c V. Paskova, K. Chudejova, A. Sramkova, L. Kraftova, V. Jakubu, EA. Petinaki, H. Zemlickova, K. Neradova, CC. Papagiannitsis, J. Hrabak
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$a Rapid identification of methicillin-resistant Staphylococcus aureus (MRSA) is essential for proper initial antibiotic therapy and timely set up of hygienic measures. Recently, detection of MRSA using MALDI-TOF mass spectrometer mediated by the peptide-phenol-soluble modulin (PSM-mec)-linked to the class A mec gene complex present in SCCmec cassettes types II, III, and VIII of MRSA strains, has been commercially available. We present here a multicentre study on MALDI-TOF MS detection of MRSA evincing a poor repeatability and reproducibility of the assay. The sensitivity of the assay varies between 50 and 90% in strains carrying psmMEC and psmδ genes encoding for PSM-mec and δ-toxin (a member of the PSM peptide family), respectively. No false positive results were found. The very major error calculation was 30% and the major error achieved 0%. Interlaboratory repeatability varies between 0 and 100%. No significant difference was observed with the use of different cultivation media. Our data showed a poor sensitivity of the method excluding it from the use in routine laboratory testing.
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$a Chudejova, Katerina $u Department of Microbiology, Faculty of Medicine and University Hospital in Pilsen, Charles University, Pilsen, Czech Republic ; Biomedical Center, Faculty of Medicine, Charles University, Pilsen, Czech Republic
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$a Sramkova, Anna $u Department of Microbiology, Faculty of Medicine and University Hospital in Pilsen, Charles University, Pilsen, Czech Republic ; Biomedical Center, Faculty of Medicine, Charles University, Pilsen, Czech Republic
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$a Kraftova, Lucie $u Department of Microbiology, Faculty of Medicine and University Hospital in Pilsen, Charles University, Pilsen, Czech Republic ; Biomedical Center, Faculty of Medicine, Charles University, Pilsen, Czech Republic
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$a Jakubu, Vladislav $u Biomedical Center, Faculty of Medicine, Charles University, Pilsen, Czech Republic ; National Reference Laboratory for Antibiotics, National Institute of Public Health, Prague, Czech Republic ; Charles University, Hradec Kralove, Czech Republic
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$a Petinaki, Efthimia A $u Department of Microbiology, University Hospital of Larissa, Larissa, Greece
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$a Zemlickova, Helena $u Biomedical Center, Faculty of Medicine, Charles University, Pilsen, Czech Republic $u National Reference Laboratory for Antibiotics, National Institute of Public Health, Prague, Czech Republic $u Department of Clinical Microbiology, University Hospital and Faculty of Medicine in Hradec Kralove, Charles University, Hradec Kralove, Czech Republic
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$a Neradova, Katerina $u Department of Clinical Microbiology, University Hospital and Faculty of Medicine in Hradec Kralove, Charles University, Hradec Kralove, Czech Republic
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$a Papagiannitsis, Costas C $u Department of Microbiology, Faculty of Medicine and University Hospital in Pilsen, Charles University, Pilsen, Czech Republic ; Biomedical Center, Faculty of Medicine, Charles University, Pilsen, Czech Republic
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$a Hrabak, Jaroslav $u Department of Microbiology, Faculty of Medicine and University Hospital in Pilsen, Charles University, Pilsen, Czech Republic. Jaroslav.Hrabak@lfp.cuni.cz ; Biomedical Center, Faculty of Medicine, Charles University, Pilsen, Czech Republic. Jaroslav.Hrabak@lfp.cuni.cz
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