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Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results
P. Møller, A. Azqueta, E. Boutet-Robinet, G. Koppen, S. Bonassi, M. Milić, G. Gajski, S. Costa, JP. Teixeira, C. Costa Pereira, M. Dusinska, R. Godschalk, G. Brunborg, KB. Gutzkow, L. Giovannelli, MS. Cooke, E. Richling, B. Laffon, V....
Language English Country Great Britain
Document type Journal Article, Review
NLK
ProQuest Central
from 2006-06-01 to 1 year ago
Health & Medicine (ProQuest)
from 2006-06-01 to 1 year ago
- MeSH
- Guideline Adherence statistics & numerical data MeSH
- Comet Assay methods standards MeSH
- Consensus MeSH
- Laboratories MeSH
- Humans MeSH
- Research Design * MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Review MeSH
The comet assay is a widely used test for the detection of DNA damage and repair activity. However, there are interlaboratory differences in reported levels of baseline and induced damage in the same experimental systems. These differences may be attributed to protocol differences, although it is difficult to identify the relevant conditions because detailed comet assay procedures are not always published. Here, we present a Consensus Statement for the Minimum Information for Reporting Comet Assay (MIRCA) providing recommendations for describing comet assay conditions and results. These recommendations differentiate between 'desirable' and 'essential' information: 'essential' information refers to the precise details that are necessary to assess the quality of the experimental work, whereas 'desirable' information relates to technical issues that might be encountered when repeating the experiments. Adherence to MIRCA recommendations should ensure that comet assay results can be easily interpreted and independently verified by other researchers.
Biomedical Center Medical Faculty in Pilsen Charles University Prague Prague Czech Republic
Centre for Environmental Sciences Hasselt University Hasselt Belgium
Department of Genetic Toxicology and Cancer Biology National Institute of Biology Ljubljana Slovenia
Department of Human Sciences and Quality of Life Promotion San Raffaele University Rome Italy
Department of Nutrition University of Oslo Oslo Norway
Department of Pharmacology and Toxicology University of Navarra Pamplona Spain
Department of Toxicology Faculty of Pharmacy Hacettepe University Ankara Turkey
Environmental Health Department National Institute of Health Dr Ricardo Jorge Porto Portugal
Epidemiological Research Unit Instituto de Saúde Pública Universidade do Porto Porto Portugal
IdiSNA Navarra Institute for Health Research Pamplona Spain
Institute of Experimental Medicine Czech Academy of Sciences Prague Czech Republic
Institute of Pharmacology and Toxicology University of Würzburg Würzburg Germany
Instituto de Investigación Biomédica de A Coruña AE CICA INIBIC Coruña Spain
Laboratory of Translational Biomedicine University of Southern Santa Catarina UNESC Criciúma Brazil
Mutagenesis Unit Institute for Medical Research and Occupational Health Zagreb Croatia
Toxalim Université de Toulouse INRAE ENVT INP Purpan UPS Toulouse France
Unit of Clinical and Molecular Epidemiology IRCCS San Raffaele Pisana Rome Italy
References provided by Crossref.org
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- $a Møller, Peter $u Department of Public Health, Section of Environmental Health, University of Copenhagen, Copenhagen, Denmark. pemo@sund.ku.dk
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