• Something wrong with this record ?

Comparison of Dietary Oils with Different Polyunsaturated Fatty Acid n-3 and n-6 Content in the Rat Model of Cutaneous Wound Healing

T. Komprda, Z. Sladek, Z. Sevcikova, V. Svehlova, J. Wijacki, R. Guran, T. Do, Z. Lackova, H. Polanska, L. Vrlikova, V. Popelkova, P. Michalek, O. Zitka, M. Buchtova

. 2020 ; 21 (21) : . [pub] 20201024

Language English Country Switzerland

Document type Comparative Study, Journal Article

Persistent link   https://www.medvik.cz/link/bmc21011990

Grant support
AF-IGA 2019-TP006 Internal Grant Agency of Mendel University in Brno
LQ1601 CEITEC 2020

Dietary supplementation with polyunsaturated fatty acids (PUFA) n-3 can affect cutaneous wound healing; however, recent findings demonstrate the variable extent of their influence on the quality of healing. Here, we compare the effect of several dietary oils, containing different levels of PUFA n-3 and PUFA n-6, on wound healing in the rat model. Rats were fed the feed mixture with 8% palm oil (P), safflower oil (S), fish oil (F) or Schizochytrium microalga extract (Sch) and compared to the animals fed by control feed mixture (C). Dorsal full-thickness cutaneous excisions were performed after 52 days of feeding and skin was left to heal for an additional 12 days. Histopathological analysis of skin wounds was performed, including immune cells immunolabeling and the determination of hydroxyproline amount as well as gene expression analyses of molecules contributing to different steps of the healing. Matrix-assisted-laser-desorption-ionization mass-spectrometry-imaging (MALDI-MSI) was used to determine the amount of collagen α-1(III) chain fragment in healing samples. Treatment by Schizochytrium extract resulted in decrease in the total wound area, in contrast to the safflower oil group where the size of the wound was larger when comparing to control animals. Diet with Schizochytrium extract and safflower oils displayed a tendency to increase the number of new vessels. The number of MPO-positive cells was diminished following any of oil treatment in comparison to the control, but their highest amount was found in animals with a fish oil diet. On the other hand, the number of CD68-positive macrophages was increased, with the most significant enhancement in the fish oil and safflower oil group. Hydroxyproline concentration was the highest in the safflower oil group but it was also enhanced in all other analyzed treatments in comparison to the control. MALDI-MSI signal intensity of a collagen III fragment decreased in the sequence C > S > Sch > P > F treatment. In conclusion, we observed differences in tissue response during healing between dietary oils, with the activation of inflammation observed following the treatment with oil containing high eicosapentaenoic acid (EPA) level (fish oil) and enhanced healing features were induced by the diet with high content of docosahexaenoic acid (DHA, Schizochytrium extract).

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc21011990
003      
CZ-PrNML
005      
20210507102734.0
007      
ta
008      
210420s2020 sz f 000 0|eng||
009      
AR
024    7_
$a 10.3390/ijms21217911 $2 doi
035    __
$a (PubMed)33114430
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a sz
100    1_
$a Komprda, Tomas $u Department of Food Technology, Mendel University in Brno, Zemedelska 1, 61300 Brno, Czech Republic
245    10
$a Comparison of Dietary Oils with Different Polyunsaturated Fatty Acid n-3 and n-6 Content in the Rat Model of Cutaneous Wound Healing / $c T. Komprda, Z. Sladek, Z. Sevcikova, V. Svehlova, J. Wijacki, R. Guran, T. Do, Z. Lackova, H. Polanska, L. Vrlikova, V. Popelkova, P. Michalek, O. Zitka, M. Buchtova
520    9_
$a Dietary supplementation with polyunsaturated fatty acids (PUFA) n-3 can affect cutaneous wound healing; however, recent findings demonstrate the variable extent of their influence on the quality of healing. Here, we compare the effect of several dietary oils, containing different levels of PUFA n-3 and PUFA n-6, on wound healing in the rat model. Rats were fed the feed mixture with 8% palm oil (P), safflower oil (S), fish oil (F) or Schizochytrium microalga extract (Sch) and compared to the animals fed by control feed mixture (C). Dorsal full-thickness cutaneous excisions were performed after 52 days of feeding and skin was left to heal for an additional 12 days. Histopathological analysis of skin wounds was performed, including immune cells immunolabeling and the determination of hydroxyproline amount as well as gene expression analyses of molecules contributing to different steps of the healing. Matrix-assisted-laser-desorption-ionization mass-spectrometry-imaging (MALDI-MSI) was used to determine the amount of collagen α-1(III) chain fragment in healing samples. Treatment by Schizochytrium extract resulted in decrease in the total wound area, in contrast to the safflower oil group where the size of the wound was larger when comparing to control animals. Diet with Schizochytrium extract and safflower oils displayed a tendency to increase the number of new vessels. The number of MPO-positive cells was diminished following any of oil treatment in comparison to the control, but their highest amount was found in animals with a fish oil diet. On the other hand, the number of CD68-positive macrophages was increased, with the most significant enhancement in the fish oil and safflower oil group. Hydroxyproline concentration was the highest in the safflower oil group but it was also enhanced in all other analyzed treatments in comparison to the control. MALDI-MSI signal intensity of a collagen III fragment decreased in the sequence C > S > Sch > P > F treatment. In conclusion, we observed differences in tissue response during healing between dietary oils, with the activation of inflammation observed following the treatment with oil containing high eicosapentaenoic acid (EPA) level (fish oil) and enhanced healing features were induced by the diet with high content of docosahexaenoic acid (DHA, Schizochytrium extract).
650    _2
$a zvířata $7 D000818
650    _2
$a antigeny CD8 $x metabolismus $7 D016827
650    _2
$a kolagen typ III $x metabolismus $7 D024061
650    _2
$a dietní tuky nenasycené $x aplikace a dávkování $x farmakologie $7 D004042
650    _2
$a modely nemocí na zvířatech $7 D004195
650    _2
$a omega-3 mastné kyseliny $x analýza $7 D015525
650    _2
$a kyseliny mastné omega-6 $x analýza $7 D043371
650    _2
$a rybí oleje $x aplikace a dávkování $x chemie $x farmakologie $7 D005395
650    _2
$a indoly $x chemie $7 D007211
650    _2
$a makrofágy $x imunologie $7 D008264
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a palmový olej $x aplikace a dávkování $x chemie $x farmakologie $7 D000073878
650    _2
$a krysa rodu Rattus $7 D051381
650    _2
$a světlicový olej $x aplikace a dávkování $x chemie $x farmakologie $7 D012450
650    _2
$a kůže $x účinky léků $x zranění $x metabolismus $7 D012867
650    _2
$a spektrometrie hmotnostní - ionizace laserem za účasti matrice $7 D019032
650    _2
$a hojení ran $x účinky léků $7 D014945
655    _2
$a srovnávací studie $7 D003160
655    _2
$a časopisecké články $7 D016428
700    1_
$a Sladek, Zbysek $u Department of Animal Morphology, Physiology and Genetics, Mendel University in Brno, Zemedelska 1, 61300 Brno, Czech Republic
700    1_
$a Sevcikova, Zuzana $u Laboratory of Molecular Morphogenesis, Institute of Animal Physiology and Genetics, v.v.i., Czech Academy of Sciences, Brno, Veveri 97, 60200 Brno, Czech Republic
700    1_
$a Svehlova, Veronika $u Department of Food Technology, Mendel University in Brno, Zemedelska 1, 61300 Brno, Czech Republic
700    1_
$a Wijacki, Jan $u Department of Animal Morphology, Physiology and Genetics, Mendel University in Brno, Zemedelska 1, 61300 Brno, Czech Republic
700    1_
$a Guran, Roman $u Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, 61300 Brno, Czech Republic $u Central European Institute of Technology, Brno University of Technology, Purkynova 123, 61200 Brno, Czech Republic
700    1_
$a Do, Tomas $u Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, 61300 Brno, Czech Republic
700    1_
$a Lackova, Zuzana $u Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, 61300 Brno, Czech Republic $u Central European Institute of Technology, Brno University of Technology, Purkynova 123, 61200 Brno, Czech Republic
700    1_
$a Polanska, Hana $u Department of Pathological Physiology, Faculty of Medicine, Masaryk University, Kamenice 5, 62500 Brno, Czech Republic
700    1_
$a Vrlikova, Lucie $u Laboratory of Molecular Morphogenesis, Institute of Animal Physiology and Genetics, v.v.i., Czech Academy of Sciences, Brno, Veveri 97, 60200 Brno, Czech Republic
700    1_
$a Popelkova, Vendula $u Department of Food Technology, Mendel University in Brno, Zemedelska 1, 61300 Brno, Czech Republic
700    1_
$a Michalek, Petr $u Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, 61300 Brno, Czech Republic $u Central European Institute of Technology, Brno University of Technology, Purkynova 123, 61200 Brno, Czech Republic
700    1_
$a Zitka, Ondrej $u Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, 61300 Brno, Czech Republic $u Central European Institute of Technology, Brno University of Technology, Purkynova 123, 61200 Brno, Czech Republic
700    1_
$a Buchtova, Marcela $u Laboratory of Molecular Morphogenesis, Institute of Animal Physiology and Genetics, v.v.i., Czech Academy of Sciences, Brno, Veveri 97, 60200 Brno, Czech Republic $u Section of Animal Physiology and Immunology, Department of Experimental Biology, Faculty of Science, Masaryk University, 62500 Brno, Czech Republic
773    0_
$w MED00176142 $t International journal of molecular sciences $x 1422-0067 $g Roč. 21, č. 21 (2020)
856    41
$u https://pubmed.ncbi.nlm.nih.gov/33114430 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20210420 $b ABA008
991    __
$a 20210507102733 $b ABA008
999    __
$a ok $b bmc $g 1650381 $s 1132369
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2020 $b 21 $c 21 $e 20201024 $i 1422-0067 $m International journal of molecular sciences $n Int J Mol Sci $x MED00176142
GRA    __
$a AF-IGA 2019-TP006 $p Internal Grant Agency of Mendel University in Brno
GRA    __
$a LQ1601 $p CEITEC 2020
LZP    __
$a Pubmed-20210420

Find record

Citation metrics

Archiving options