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Discovery of Unusual Cyanobacterial Tryptophan-Containing Anabaenopeptins by MS/MS-Based Molecular Networking
S. Saha, G. Esposito, P. Urajová, J. Mareš, D. Ewe, A. Caso, M. Macho, K. Delawská, A. Kust, P. Hrouzek, J. Juráň, V. Costantino, K. Saurav
Jazyk angličtina Země Švýcarsko
Typ dokumentu časopisecké články
Grantová podpora
19-17868Y
Grantová Agentura České Republiky
CZ.02.2.69/0.0/0.0/18_070/0010493
Ministerstvo Školství, Mládeže a Tělovýchovy
Campania Oncoterapie No. B61G18000470007.
Regione Campania
NLK
Directory of Open Access Journals
od 1997
Free Medical Journals
od 1997
PubMed Central
od 2001
Europe PubMed Central
od 2001
ProQuest Central
od 1997-01-01
Open Access Digital Library
od 1997-01-01
Medline Complete (EBSCOhost)
od 2009-03-01
Health & Medicine (ProQuest)
od 1997-01-01
- MeSH
- cyklické peptidy * chemie genetika izolace a purifikace farmakologie MeSH
- HeLa buňky MeSH
- hmotnostní spektrometrie MeSH
- lidé MeSH
- nukleární magnetická rezonance biomolekulární MeSH
- proliferace buněk účinky léků MeSH
- sinice * chemie genetika MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
Heterocytous cyanobacteria are among the most prolific sources of bioactive secondary metabolites, including anabaenopeptins (APTs). A terrestrial filamentous Brasilonema sp. CT11 collected in Costa Rica bamboo forest as a black mat, was studied using a multidisciplinary approach: genome mining and HPLC-HRMS/MS coupled with bioinformatic analyses. Herein, we report the nearly complete genome consisting of 8.79 Mbp with a GC content of 42.4%. Moreover, we report on three novel tryptophan-containing APTs; anabaenopeptin 788 (1), anabaenopeptin 802 (2), and anabaenopeptin 816 (3). Furthermore, the structure of two homologues, i.e., anabaenopeptin 802 (2a) and anabaenopeptin 802 (2b), was determined by spectroscopic analysis (NMR and MS). Both compounds were shown to exert weak to moderate antiproliferative activity against HeLa cell lines. This study also provides the unique and diverse potential of biosynthetic gene clusters and an assessment of the predicted chemical space yet to be discovered from this genus.
Institute of Botany of the Czech Academy of Sciences 252 43 Průhonice Czech Republic
Task Force Big Fed2 The Blue Chemistry lab Università degli Studi di Napoli 80131 Napoli Italy
Citace poskytuje Crossref.org
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