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Non-apoptotic functions of caspase-7 during osteogenesis
E. Svandova, H. Lesot, T. Vanden Berghe, AS. Tucker, PT. Sharpe, P. Vandenabeele, E. Matalova,
Jazyk angličtina Země Anglie, Velká Británie
Typ dokumentu časopisecké články, práce podpořená grantem
NLK
Directory of Open Access Journals
od 2010
Free Medical Journals
od 2010
Freely Accessible Science Journals
od 2010
PubMed Central
od 2010
Europe PubMed Central
od 2010
ProQuest Central
od 2010-01-01 do 2017-12-31
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od 2010-01-01
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od 2010-01-01
Open Access Digital Library
od 2010-01-01
Health & Medicine (ProQuest)
od 2010-01-01 do 2017-12-31
ROAD: Directory of Open Access Scholarly Resources
od 2010
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od 2010-01-01
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od 2010-01-01
PubMed
25118926
DOI
10.1038/cddis.2014.330
Knihovny.cz E-zdroje
- MeSH
- apoptóza MeSH
- embryonální vývoj MeSH
- kaspasa 3 metabolismus MeSH
- kaspasa 7 genetika metabolismus MeSH
- kosti a kostní tkáň metabolismus patologie radiografie MeSH
- myši knockoutované MeSH
- myši MeSH
- osteogeneze * MeSH
- osteokalcin metabolismus MeSH
- počítačová rentgenová tomografie MeSH
- protein Smad1 genetika metabolismus MeSH
- transkripční faktor MSX1 genetika metabolismus MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Caspase-3 and -7 are generally known for their central role in the execution of apoptosis. However, their function is not limited to apoptosis and under specific conditions activation has been linked to proliferation or differentiation of specialised cell types. In the present study, we followed the localisation of the activated form of caspase-7 during intramembranous (alveolar and mandibular bones) and endochondral (long bones of limbs) ossification in mice. In both bone types, the activated form of caspase-7 was detected from the beginning of ossification during embryonic development and persisted postnatally. The bone status was investigated by microCT in both wild-type and caspase-7-deficient adult mice. Intramembranous bone in mutant mice displayed a statistically significant decrease in volume while the mineral density was not altered. Conversely, endochondral bone showed constant volume but a significant decrease in mineral density in caspase-7 knock-out mice. Cleaved caspase-7 was present in a number of cells that did not show signs of apoptosis. PCR array analysis of the mandibular bone of caspase-7-deficient versus wild-type mice pointed to a significant decrease in mRNA levels for Msx1 and Smad1 in early bone formation. These observations might explain the decrease in the alveolar bone volume of adult knock-out mice. In conclusion, this study is the first to report a non-apoptotic function of caspase-7 in osteogenesis and also demonstrates further specificities in endochondral versus intramembranous ossification.
Citace poskytuje Crossref.org
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