-
Something wrong with this record ?
Non-apoptotic functions of caspase-7 during osteogenesis
E. Svandova, H. Lesot, T. Vanden Berghe, AS. Tucker, PT. Sharpe, P. Vandenabeele, E. Matalova,
Language English Country England, Great Britain
Document type Journal Article, Research Support, Non-U.S. Gov't
NLK
Directory of Open Access Journals
from 2010
Free Medical Journals
from 2010
Freely Accessible Science Journals
from 2010
Nature Open Access
from 2010-01-01
PubMed Central
from 2010
Europe PubMed Central
from 2010
ProQuest Central
from 2010-01-01
Open Access Digital Library
from 2010-01-01
Open Access Digital Library
from 2010-01-01
Open Access Digital Library
from 2010-01-01
Health & Medicine (ProQuest)
from 2010-01-01
ROAD: Directory of Open Access Scholarly Resources
from 2010
Springer Nature OA/Free Journals
from 2010-01-01
- MeSH
- Apoptosis MeSH
- Embryonic Development MeSH
- Caspase 3 metabolism MeSH
- Caspase 7 genetics metabolism MeSH
- Bone and Bones metabolism pathology radiography MeSH
- Mice, Knockout MeSH
- Mice MeSH
- Osteogenesis * MeSH
- Osteocalcin metabolism MeSH
- Tomography, X-Ray Computed MeSH
- Smad1 Protein genetics metabolism MeSH
- MSX1 Transcription Factor genetics metabolism MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Caspase-3 and -7 are generally known for their central role in the execution of apoptosis. However, their function is not limited to apoptosis and under specific conditions activation has been linked to proliferation or differentiation of specialised cell types. In the present study, we followed the localisation of the activated form of caspase-7 during intramembranous (alveolar and mandibular bones) and endochondral (long bones of limbs) ossification in mice. In both bone types, the activated form of caspase-7 was detected from the beginning of ossification during embryonic development and persisted postnatally. The bone status was investigated by microCT in both wild-type and caspase-7-deficient adult mice. Intramembranous bone in mutant mice displayed a statistically significant decrease in volume while the mineral density was not altered. Conversely, endochondral bone showed constant volume but a significant decrease in mineral density in caspase-7 knock-out mice. Cleaved caspase-7 was present in a number of cells that did not show signs of apoptosis. PCR array analysis of the mandibular bone of caspase-7-deficient versus wild-type mice pointed to a significant decrease in mRNA levels for Msx1 and Smad1 in early bone formation. These observations might explain the decrease in the alveolar bone volume of adult knock-out mice. In conclusion, this study is the first to report a non-apoptotic function of caspase-7 in osteogenesis and also demonstrates further specificities in endochondral versus intramembranous ossification.
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc16010754
- 003
- CZ-PrNML
- 005
- 20160412124346.0
- 007
- ta
- 008
- 160408s2014 enk f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1038/cddis.2014.330 $2 doi
- 024 7_
- $a 10.1038/cddis.2014.330 $2 doi
- 035 __
- $a (PubMed)25118926
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a enk
- 100 1_
- $a Svandova, E $u 1] Institute of Animal Physiology and Genetics CAS, v.v.i., Brno, Czech Republic [2] Department of Physiology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic.
- 245 10
- $a Non-apoptotic functions of caspase-7 during osteogenesis / $c E. Svandova, H. Lesot, T. Vanden Berghe, AS. Tucker, PT. Sharpe, P. Vandenabeele, E. Matalova,
- 520 9_
- $a Caspase-3 and -7 are generally known for their central role in the execution of apoptosis. However, their function is not limited to apoptosis and under specific conditions activation has been linked to proliferation or differentiation of specialised cell types. In the present study, we followed the localisation of the activated form of caspase-7 during intramembranous (alveolar and mandibular bones) and endochondral (long bones of limbs) ossification in mice. In both bone types, the activated form of caspase-7 was detected from the beginning of ossification during embryonic development and persisted postnatally. The bone status was investigated by microCT in both wild-type and caspase-7-deficient adult mice. Intramembranous bone in mutant mice displayed a statistically significant decrease in volume while the mineral density was not altered. Conversely, endochondral bone showed constant volume but a significant decrease in mineral density in caspase-7 knock-out mice. Cleaved caspase-7 was present in a number of cells that did not show signs of apoptosis. PCR array analysis of the mandibular bone of caspase-7-deficient versus wild-type mice pointed to a significant decrease in mRNA levels for Msx1 and Smad1 in early bone formation. These observations might explain the decrease in the alveolar bone volume of adult knock-out mice. In conclusion, this study is the first to report a non-apoptotic function of caspase-7 in osteogenesis and also demonstrates further specificities in endochondral versus intramembranous ossification.
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a apoptóza $7 D017209
- 650 _2
- $a kosti a kostní tkáň $x metabolismus $x patologie $x radiografie $7 D001842
- 650 _2
- $a kaspasa 3 $x metabolismus $7 D053148
- 650 _2
- $a kaspasa 7 $x genetika $x metabolismus $7 D053179
- 650 _2
- $a embryonální vývoj $7 D047108
- 650 _2
- $a transkripční faktor MSX1 $x genetika $x metabolismus $7 D051957
- 650 _2
- $a myši $7 D051379
- 650 _2
- $a myši knockoutované $7 D018345
- 650 _2
- $a osteokalcin $x metabolismus $7 D015675
- 650 12
- $a osteogeneze $7 D010012
- 650 _2
- $a protein Smad1 $x genetika $x metabolismus $7 D051898
- 650 _2
- $a počítačová rentgenová tomografie $7 D014057
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Lesot, H $u 1] INSERM UMR 1109, team "Osteoarticular and Dental Regenerative NanoMedicine", Strasbourg, France [2] Faculté de chirurgie dentaire, Université de Strasbourg, Strasbourg, France.
- 700 1_
- $a Vanden Berghe, T $u 1] Inflammation Research Centre, VIB, Ghent, Belgium [2] Department of Molecular Biology, Ghent University, Ghent, Belgium.
- 700 1_
- $a Tucker, A S $u Department of Craniofacial Development and Stem Cell Biology, King's College London, London, UK.
- 700 1_
- $a Sharpe, P T $u Department of Craniofacial Development and Stem Cell Biology, King's College London, London, UK.
- 700 1_
- $a Vandenabeele, P $u 1] Inflammation Research Centre, VIB, Ghent, Belgium [2] Department of Molecular Biology, Ghent University, Ghent, Belgium.
- 700 1_
- $a Matalova, E $u 1] Institute of Animal Physiology and Genetics CAS, v.v.i., Brno, Czech Republic [2] Department of Physiology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic.
- 773 0_
- $w MED00173233 $t Cell death & disease $x 2041-4889 $g Roč. 5, č. - (2014), s. e1366
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/25118926 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20160408 $b ABA008
- 991 __
- $a 20160412124429 $b ABA008
- 999 __
- $a ok $b bmc $g 1114183 $s 935122
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2014 $b 5 $c - $d e1366 $e 20140814 $i 2041-4889 $m Cell death & disease $n Cell Death Dis $x MED00173233
- LZP __
- $a Pubmed-20160408
