-
Something wrong with this record ?
Sensitive Fluorescence In Situ Hybridization on Semithin Sections of Adult Schistosoma mansoni Using DIG-Labeled RNA Probes
L. Ulrychová, M. Horn, J. Dvořák
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Digoxigenin metabolism MeSH
- In Situ Hybridization, Fluorescence methods MeSH
- RNA, Messenger genetics metabolism MeSH
- RNA Probes metabolism MeSH
- Schistosoma mansoni cytology MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
In situ hybridization is a tool for evaluation of gene expression within tissues or single cells. This protocol describes optimized sensitive fluorescence detection of gene transcripts (mRNAs) in semithin sections of Schistosoma mansoni adult worms using specifically designed and labeled RNA probes. Due to improved methodologies in tissue preservation, sectioning, amplification of fluorescent signal, and prehybridization tissue treatment, it is possible to detect transcripts in the fine structures of schistosomes. The protocol is sensitive enough to detect very low abundance targets. This procedure is optimized for tissues derived from S. mansoni adult worms; however, it can be successfully applied to other trematode species.
Department of Parasitology Faculty of Science Charles University Prague Czech Republic
Institute of Organic Chemistry and Biochemistry Czech Academy of Sciences Prague Czech Republic
Institute of Specific Prophylaxis and Tropical Medicine Medical University of Vienna Vienna Austria
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc21012978
- 003
- CZ-PrNML
- 005
- 20210716111000.0
- 007
- ta
- 008
- 210420s2020 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1007/978-1-0716-0635-3_4 $2 doi
- 035 __
- $a (PubMed)32451994
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Ulrychová, Lenka $u Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Prague, Czech Republic ; Department of Parasitology, Faculty of Science, Charles University, Prague, Czech Republic
- 245 10
- $a Sensitive Fluorescence In Situ Hybridization on Semithin Sections of Adult Schistosoma mansoni Using DIG-Labeled RNA Probes / $c L. Ulrychová, M. Horn, J. Dvořák
- 520 9_
- $a In situ hybridization is a tool for evaluation of gene expression within tissues or single cells. This protocol describes optimized sensitive fluorescence detection of gene transcripts (mRNAs) in semithin sections of Schistosoma mansoni adult worms using specifically designed and labeled RNA probes. Due to improved methodologies in tissue preservation, sectioning, amplification of fluorescent signal, and prehybridization tissue treatment, it is possible to detect transcripts in the fine structures of schistosomes. The protocol is sensitive enough to detect very low abundance targets. This procedure is optimized for tissues derived from S. mansoni adult worms; however, it can be successfully applied to other trematode species.
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a digoxigenin $x metabolismus $7 D004076
- 650 _2
- $a hybridizace in situ fluorescenční $x metody $7 D017404
- 650 _2
- $a RNA sondy $x metabolismus $7 D015347
- 650 _2
- $a messenger RNA $x genetika $x metabolismus $7 D012333
- 650 _2
- $a Schistosoma mansoni $x cytologie $7 D012550
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Horn, Martin $u Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Prague, Czech Republic
- 700 1_
- $a Dvořák, Jan $u Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Prague, Czech Republic. dvorak19@af.czu.cz ; Department of Zoology and Fisheries, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences in Prague, Prague, Czech Republic. dvorak19@af.czu.cz ; Institute of Specific Prophylaxis and Tropical Medicine, Medical University of Vienna, Vienna, Austria. dvorak19@af.czu.cz
- 773 0_
- $w MED00180389 $t Methods in molecular biology (Clifton, N.J.) $x 1940-6029 $g Roč. 2151, č. - (2020), s. 43-53
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/32451994 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y p $z 0
- 990 __
- $a 20210420 $b ABA008
- 991 __
- $a 20210716111000 $b ABA008
- 999 __
- $a ok $b bmc $g 1651207 $s 1133357
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2020 $b 2151 $c - $d 43-53 $e - $i 1940-6029 $m Methods in molecular biology $n Methods Mol Biol $x MED00180389
- LZP __
- $a Pubmed-20210420
