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Multiplexed digital polymerase chain reaction as a powerful diagnostic tool
M. Gaňová, H. Zhang, H. Zhu, M. Korabečná, P. Neužil
Language English Country Great Britain
Document type Journal Article, Review
- MeSH
- Biosensing Techniques * MeSH
- Polymerase Chain Reaction MeSH
- Publication type
- Journal Article MeSH
- Review MeSH
The digital polymerase chain reaction (dPCR) multiplexing method can simultaneously detect and quantify closely related deoxyribonucleic acid sequences in complex mixtures. The dPCR concept is continuously improved by the development of microfluidics and micro- and nanofabrication, and different complex techniques are introduced. In this review, we introduce dPCR techniques based on sample compartmentalization, droplet- and chip-based systems, and their combinations. We then discuss dPCR multiplexing methods in both laboratory research settings and advanced or routine clinical applications. We focus on their strengths and weaknesses with regard to the character of biological samples and to the required precision of such analysis, as well as showing recently published work based on those methods. Finally, we envisage possible future achievements in this field.
Central European Institute of Technology Brno University of Technology 612 00 Brno Czech Republic
School of Mechanical Engineering Northwestern Polytechnical University Xi'an 710072 PR China
References provided by Crossref.org
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- $a The digital polymerase chain reaction (dPCR) multiplexing method can simultaneously detect and quantify closely related deoxyribonucleic acid sequences in complex mixtures. The dPCR concept is continuously improved by the development of microfluidics and micro- and nanofabrication, and different complex techniques are introduced. In this review, we introduce dPCR techniques based on sample compartmentalization, droplet- and chip-based systems, and their combinations. We then discuss dPCR multiplexing methods in both laboratory research settings and advanced or routine clinical applications. We focus on their strengths and weaknesses with regard to the character of biological samples and to the required precision of such analysis, as well as showing recently published work based on those methods. Finally, we envisage possible future achievements in this field.
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- $a Neužil, Pavel $u Central European Institute of Technology, Brno University of Technology, 612 00, Brno, Czech Republic; School of Mechanical Engineering, Northwestern Polytechnical University, Xi'an, 710072, PR China; The Faculty of Electrical Engineering and Communication, Brno University of Technology, 616 00, Brno, Czech Republic. Electronic address: pavel.neuzil@nwpu.edu.cn
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