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Epigenetic Distribution of Recombinant Plant Chromosome Fragments in a Human-Arabidopsis Hybrid Cell Line
Y. Liaw, Y. Liu, C. Teo, P. Cápal, N. Wada, K. Fukui, J. Doležel, N. Ohmido
Language English Country Switzerland
Document type Journal Article
Grant support
CZ.02.1.01/0.0/0.0/16_019/0000827
ERDF project
JSPS-18-07
Mobility Plus Project
JPJSBP120203507
Mobility Plus Project
Innovative Asia
JICA
Kobe University Visiting Researcher project
Kobe University
NLK
Free Medical Journals
from 2000
Freely Accessible Science Journals
from 2000
PubMed Central
from 2007
Europe PubMed Central
from 2007
ProQuest Central
from 2000-03-01
Open Access Digital Library
from 2000-01-01
Open Access Digital Library
from 2007-01-01
Health & Medicine (ProQuest)
from 2000-03-01
ROAD: Directory of Open Access Scholarly Resources
from 2000
PubMed
34063996
DOI
10.3390/ijms22115426
Knihovny.cz E-resources
- MeSH
- Arabidopsis genetics MeSH
- Cell Line MeSH
- Chromosomes, Plant genetics MeSH
- DNA, Plant genetics MeSH
- Epigenesis, Genetic genetics MeSH
- Epigenome genetics MeSH
- Epigenomics methods MeSH
- Genome, Plant genetics MeSH
- Hybrid Cells physiology MeSH
- Humans MeSH
- Methyltransferases genetics MeSH
- DNA Methylation genetics MeSH
- Repetitive Sequences, Nucleic Acid genetics MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
Methylation systems have been conserved during the divergence of plants and animals, although they are regulated by different pathways and enzymes. However, studies on the interactions of the epigenomes among evolutionarily distant organisms are lacking. To address this, we studied the epigenetic modification and gene expression of plant chromosome fragments (~30 Mb) in a human-Arabidopsis hybrid cell line. The whole-genome bisulfite sequencing results demonstrated that recombinant Arabidopsis DNA could retain its plant CG methylation levels even without functional plant methyltransferases, indicating that plant DNA methylation states can be maintained even in a different genomic background. The differential methylation analysis showed that the Arabidopsis DNA was undermethylated in the centromeric region and repetitive elements. Several Arabidopsis genes were still expressed, whereas the expression patterns were not related to the gene function. We concluded that the plant DNA did not maintain the original plant epigenomic landscapes and was under the control of the human genome. This study showed how two diverging genomes can coexist and provided insights into epigenetic modifications and their impact on the regulation of gene expressions between plant and animal genomes.
Graduate School of Human Development and Environment Kobe University Kobe Hyogo 657 8501 Japan
Graduate School of Pharmaceutical Sciences Osaka University Suita Osaka 565 0871 Japan
References provided by Crossref.org
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