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Direct and Indirect Antioxidant Effects of Selected Plant Phenolics in Cell-Based Assays
J. Treml, P. Večeřová, P. Herczogová, K. Šmejkal
Language English Country Switzerland
Document type Journal Article
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- MeSH
- Antioxidant Response Elements MeSH
- Antioxidants chemistry pharmacology MeSH
- Biomarkers MeSH
- Gene Expression MeSH
- NF-E2-Related Factor 2 genetics metabolism MeSH
- Phenols chemistry pharmacology MeSH
- Glucose MeSH
- Humans MeSH
- Molecular Structure MeSH
- Oxidative Stress MeSH
- Antineoplastic Agents chemistry pharmacology MeSH
- Plant Extracts chemistry pharmacology MeSH
- Superoxide Dismutase-1 genetics metabolism MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
Background: Oxidative stress is a key factor in the pathophysiology of many diseases. This study aimed to verify the antioxidant activity of selected plant phenolics in cell-based assays and determine their direct or indirect effects. Methods: The cellular antioxidant assay (CAA) assay was employed for direct scavenging assays. In the indirect approach, the influence of each test substance on the gene and protein expression and activity of selected antioxidant enzymes was observed. One assay also dealt with activation of the Nrf2-ARE pathway. The overall effect of each compound was measured using a glucose oxidative stress protection assay. Results: Among the test compounds, acteoside showed the highest direct scavenging activity and no effect on the expression of antioxidant enzymes. It increased only the activity of catalase. Diplacone was less active in direct antioxidant assays but positively affected enzyme expression and catalase activity. Morusin showed no antioxidant activity in the CAA assay. Similarly, pomiferin had only mild antioxidant activity and proved rather cytotoxic. Conclusions: Of the four selected phenolics, only acteoside and diplacone demonstrated antioxidant effects in cell-based assays.
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