-
Something wrong with this record ?
Maternal Exposure Results in Long-Term Deoxynivalenol Persistence in Piglets' Plasma and Modulates the Immune System
H. Štěpánová, K. Hlavová, K. Šťastný, E. Gopfert, L. Levá, M. Faldyna
Language English Country Switzerland
Document type Journal Article, Research Support, Non-U.S. Gov't
Grant support
LD15055
Ministry of Education, Youth and Sports of the Czech Republic - International
CZ.1.05/2.1.00/19.0385
Ministry of Education, Youth and Sports of the Czech Republic - International
RO0518
Ministry of Agriculture of the Czech Republic - International
NLK
Directory of Open Access Journals
from 2009
Free Medical Journals
from 2009
PubMed Central
from 2009
Europe PubMed Central
from 2009
ProQuest Central
from 2009-01-01
Open Access Digital Library
from 2009-01-01
Open Access Digital Library
from 2009-01-01
Medline Complete (EBSCOhost)
from 2010-09-01
Health & Medicine (ProQuest)
from 2009-01-01
ROAD: Directory of Open Access Scholarly Resources
from 2009
- MeSH
- Time Factors MeSH
- Cytokines metabolism MeSH
- Phenotype MeSH
- Gestational Age MeSH
- Immune System drug effects immunology metabolism MeSH
- Injections, Intravenous MeSH
- Maternal-Fetal Exchange * MeSH
- Maternal Exposure MeSH
- Inflammation Mediators metabolism MeSH
- Sus scrofa MeSH
- T-Lymphocyte Subsets drug effects immunology metabolism MeSH
- Pregnancy MeSH
- Trichothecenes administration & dosage blood toxicity MeSH
- Prenatal Exposure Delayed Effects * MeSH
- Animals MeSH
- Check Tag
- Pregnancy MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Deoxynivalenol (DON)-contaminated feed represents a serious problem for pigs due to their high sensitivity to its toxicological effects. The aim of the present study was to evaluate the impact of intrauterine DON exposure on the immune system of piglets. Pure DON was intravenously administered to sows at the end of gestation (during the last 2-3 days of gestation, one dose of 300 µg per day). The plasma concentration of DON was analyzed using liquid chromatography combined with high-resolution Orbitrap-based mass spectrometry (LC-MS/MS (HR)) and selected immune parameters were monitored six times in piglets from birth to 18 weeks. DON was found in the plasma of 90% of newborn piglets at a mean concentration of 6.28 ng/mL and subsequently, at one, three, and seven weeks after birth with decreasing concentrations. Trace amounts were still present in the plasma 14 weeks after birth. Flow cytometry revealed a significant impact of DON on T lymphocyte subpopulations during the early postnatal period. Lower percentages of regulatory T cells, T helper lymphocytes, and their double positive CD4+CD8+ subset were followed by increased percentages of cytotoxic T lymphocytes and γδ T cells. The capacity to produce pro-inflammatory cytokines was also significantly lower after intrauterine DON exposure. In conclusion, this study revealed a long-term persistence of DON in the plasma of the piglets as a consequence of short-term intrauterine exposure, leading to altered immune parameters.
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc21020106
- 003
- CZ-PrNML
- 005
- 20210830101723.0
- 007
- ta
- 008
- 210728s2020 sz f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.3390/toxins12100615 $2 doi
- 035 __
- $a (PubMed)32992825
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a sz
- 100 1_
- $a Štěpánová, Hana $u Veterinary Research Institute, 62100 Brno, Czech Republic
- 245 10
- $a Maternal Exposure Results in Long-Term Deoxynivalenol Persistence in Piglets' Plasma and Modulates the Immune System / $c H. Štěpánová, K. Hlavová, K. Šťastný, E. Gopfert, L. Levá, M. Faldyna
- 520 9_
- $a Deoxynivalenol (DON)-contaminated feed represents a serious problem for pigs due to their high sensitivity to its toxicological effects. The aim of the present study was to evaluate the impact of intrauterine DON exposure on the immune system of piglets. Pure DON was intravenously administered to sows at the end of gestation (during the last 2-3 days of gestation, one dose of 300 µg per day). The plasma concentration of DON was analyzed using liquid chromatography combined with high-resolution Orbitrap-based mass spectrometry (LC-MS/MS (HR)) and selected immune parameters were monitored six times in piglets from birth to 18 weeks. DON was found in the plasma of 90% of newborn piglets at a mean concentration of 6.28 ng/mL and subsequently, at one, three, and seven weeks after birth with decreasing concentrations. Trace amounts were still present in the plasma 14 weeks after birth. Flow cytometry revealed a significant impact of DON on T lymphocyte subpopulations during the early postnatal period. Lower percentages of regulatory T cells, T helper lymphocytes, and their double positive CD4+CD8+ subset were followed by increased percentages of cytotoxic T lymphocytes and γδ T cells. The capacity to produce pro-inflammatory cytokines was also significantly lower after intrauterine DON exposure. In conclusion, this study revealed a long-term persistence of DON in the plasma of the piglets as a consequence of short-term intrauterine exposure, leading to altered immune parameters.
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a cytokiny $x metabolismus $7 D016207
- 650 _2
- $a ženské pohlaví $7 D005260
- 650 _2
- $a gestační stáří $7 D005865
- 650 _2
- $a imunitní systém $x účinky léků $x imunologie $x metabolismus $7 D007107
- 650 _2
- $a mediátory zánětu $x metabolismus $7 D018836
- 650 _2
- $a injekce intravenózní $7 D007275
- 650 _2
- $a matka - expozice noxám $7 D018811
- 650 12
- $a maternofetální výměna látek $7 D008431
- 650 _2
- $a fenotyp $7 D010641
- 650 _2
- $a těhotenství $7 D011247
- 650 12
- $a zpožděný efekt prenatální expozice $7 D011297
- 650 _2
- $a Sus scrofa $7 D034421
- 650 _2
- $a T-lymfocyty - podskupiny $x účinky léků $x imunologie $x metabolismus $7 D016176
- 650 _2
- $a časové faktory $7 D013997
- 650 _2
- $a trichotheceny $x aplikace a dávkování $x krev $x toxicita $7 D014255
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Hlavová, Karolina $u Veterinary Research Institute, 62100 Brno, Czech Republic
- 700 1_
- $a Šťastný, Kamil $u Veterinary Research Institute, 62100 Brno, Czech Republic
- 700 1_
- $a Gopfert, Eduard $u Veterinary Research Institute, 62100 Brno, Czech Republic
- 700 1_
- $a Levá, Lenka $u Veterinary Research Institute, 62100 Brno, Czech Republic
- 700 1_
- $a Faldyna, Martin $u Veterinary Research Institute, 62100 Brno, Czech Republic
- 773 0_
- $w MED00177194 $t Toxins $x 2072-6651 $g Roč. 12, č. 10 (2020)
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/32992825 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y p $z 0
- 990 __
- $a 20210728 $b ABA008
- 991 __
- $a 20210830101723 $b ABA008
- 999 __
- $a ok $b bmc $g 1690820 $s 1140552
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2020 $b 12 $c 10 $e 20200925 $i 2072-6651 $m Toxins $n Toxins $x MED00177194
- GRA __
- $a LD15055 $p Ministry of Education, Youth and Sports of the Czech Republic $2 International
- GRA __
- $a CZ.1.05/2.1.00/19.0385 $p Ministry of Education, Youth and Sports of the Czech Republic $2 International
- GRA __
- $a RO0518 $p Ministry of Agriculture of the Czech Republic $2 International
- LZP __
- $a Pubmed-20210728