• Je něco špatně v tomto záznamu ?

AGR2-AGR3 hetero-oligomeric complexes: Identification and characterization

H. Černocká, P. Vonka, V. Kasalová, L. Sommerova, V. Vandova, R. Hrstka, V. Ostatna

. 2021 ; 140 (-) : 107808. [pub] 20210326

Jazyk angličtina Země Nizozemsko

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc21025183

In this paper we compare electrochemical behavior of two homolog proteins, namely anterior gradient 2 (AGR2) and anterior gradient 3 (AGR3), playing an important role in cancer cell biology. The slight variation in their protein structures has an impact on protein adsorption and orientation at charged surface and also enables AGR2 and AGR3 to form heterocomplexes. We confirm interaction between AGR2 and AGR3 (i) in vitro by immunochemical and constant current chronopotentiometric stripping (CPS) analysis and (ii) in vivo by bioluminescence resonance energy transfer (BRET) assay. Mutation of AGR2 in dimerization domain (E60A) prevents development of wild type AGR2 dimers and also negatively affects interaction with wild type AGR3 as shown by CPS analysis. Beside new information about AGR2 and AGR3 protein including their joint interaction, our work introduces possible applications of CPS in bioanalysis of protein complexes, including those relatively unstable, but important in the cancer research.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc21025183
003      
CZ-PrNML
005      
20211026134039.0
007      
ta
008      
211013s2021 ne f 000 0|eng||
009      
AR
024    7_
$a 10.1016/j.bioelechem.2021.107808 $2 doi
035    __
$a (PubMed)33848875
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a ne
100    1_
$a Černocká, Hana $u Institute of Biophysics, The Czech Academy of Sciences, v.v.i., Královopolská 135, 61265 Brno, Czech Republic
245    10
$a AGR2-AGR3 hetero-oligomeric complexes: Identification and characterization / $c H. Černocká, P. Vonka, V. Kasalová, L. Sommerova, V. Vandova, R. Hrstka, V. Ostatna
520    9_
$a In this paper we compare electrochemical behavior of two homolog proteins, namely anterior gradient 2 (AGR2) and anterior gradient 3 (AGR3), playing an important role in cancer cell biology. The slight variation in their protein structures has an impact on protein adsorption and orientation at charged surface and also enables AGR2 and AGR3 to form heterocomplexes. We confirm interaction between AGR2 and AGR3 (i) in vitro by immunochemical and constant current chronopotentiometric stripping (CPS) analysis and (ii) in vivo by bioluminescence resonance energy transfer (BRET) assay. Mutation of AGR2 in dimerization domain (E60A) prevents development of wild type AGR2 dimers and also negatively affects interaction with wild type AGR3 as shown by CPS analysis. Beside new information about AGR2 and AGR3 protein including their joint interaction, our work introduces possible applications of CPS in bioanalysis of protein complexes, including those relatively unstable, but important in the cancer research.
650    _2
$a adsorpce $7 D000327
650    _2
$a transportní proteiny $x chemie $7 D002352
650    _2
$a lidé $7 D006801
650    _2
$a molekulární modely $7 D008958
650    _2
$a mukoproteiny $x chemie $7 D009088
650    _2
$a nádorové proteiny $x chemie $7 D009363
650    _2
$a onkogenní proteiny $x chemie $7 D015513
650    _2
$a proteinové domény $7 D000072417
650    12
$a multimerizace proteinu $7 D055503
650    _2
$a kvarterní struktura proteinů $7 D020836
655    _2
$a časopisecké články $7 D016428
700    1_
$a Vonka, Petr $u Masaryk Memorial Cancer Institute, Research Centre for Applied Molecular Oncology, Zluty kopec 7, 65653 Brno, Czech Republic
700    1_
$a Kasalová, Veronika $u Institute of Biophysics, The Czech Academy of Sciences, v.v.i., Královopolská 135, 61265 Brno, Czech Republic
700    1_
$a Sommerova, Lucia $u Masaryk Memorial Cancer Institute, Research Centre for Applied Molecular Oncology, Zluty kopec 7, 65653 Brno, Czech Republic
700    1_
$a Vandova, Veronika $u Masaryk Memorial Cancer Institute, Research Centre for Applied Molecular Oncology, Zluty kopec 7, 65653 Brno, Czech Republic
700    1_
$a Hrstka, Roman $u Masaryk Memorial Cancer Institute, Research Centre for Applied Molecular Oncology, Zluty kopec 7, 65653 Brno, Czech Republic
700    1_
$a Ostatna, Veronika $u Institute of Biophysics, The Czech Academy of Sciences, v.v.i., Královopolská 135, 61265 Brno, Czech Republic. Electronic address: ostatna@ibp.cz
773    0_
$w MED00005708 $t Bioelectrochemistry (Amsterdam, Netherlands) $x 1878-562X $g Roč. 140, č. - (2021), s. 107808
856    41
$u https://pubmed.ncbi.nlm.nih.gov/33848875 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20211013 $b ABA008
991    __
$a 20211026134045 $b ABA008
999    __
$a ok $b bmc $g 1714299 $s 1145690
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2021 $b 140 $c - $d 107808 $e 20210326 $i 1878-562X $m Bioelectrochemistry $n Bioelectrochemistry $x MED00005708
LZP    __
$a Pubmed-20211013
Zpět

Najít záznam

Citační ukazatele

Možnosti archivace