Classical models of gene expression were built using genetics and biochemistry. Although these approaches are powerful, they have very limited consideration of the spatial and temporal organization of gene expression. Although the spatial organization and dynamics of RNA polymerase II (RNAPII) transcription machinery have fundamental functional consequences for gene expression, its detailed studies have been abrogated by the limits of classical light microscopy for a long time. The advent of super-resolution microscopy (SRM) techniques allowed for the visualization of the RNAPII transcription machinery with nanometer resolution and millisecond precision. In this review, we summarize the recent methodological advances in SRM, focus on its application for studies of the nanoscale organization in space and time of RNAPII transcription, and discuss its consequences for the mechanistic understanding of gene expression.

Department of Biology of the Cell Nucleus Institute of Molecular Genetics of the Czech Academy of Sciences Vídeňská 1083 142 20 Prague Czech Republic

Faculty of Science Charles University Albertov 6 128 00 Prague Czech Republic

Microscopy Centre Institute of Molecular Genetics of the Czech Academy of Sciences Vídeňská 1083 142 20 Prague Czech Republic

000      

00000naa a2200000 a 4500

001      

bmc21025544

003      

CZ-PrNML

005      

20211026133722.0

007      

ta

008      

211013s2021 sz f 000 0|eng||

009      

AR

024    7_

$a 10.3390/ijms22136694 $2 doi

035    __

$a (PubMed)34206594

040    __

$a ABA008 $b cze $d ABA008 $e AACR2

041    0_

$a eng

044    __

$a sz

100    1_

$a Hoboth, Peter $u Department of Biology of the Cell Nucleus, Institute of Molecular Genetics of the Czech Academy of Sciences, Vídeňská 1083, 142 20 Prague, Czech Republic $u Faculty of Science, Charles University, Albertov 6, 128 00 Prague, Czech Republic

245    10

$a How Single-Molecule Localization Microscopy Expanded Our Mechanistic Understanding of RNA Polymerase II Transcription / $c P. Hoboth, O. Šebesta, P. Hozák

520    9_

$a Classical models of gene expression were built using genetics and biochemistry. Although these approaches are powerful, they have very limited consideration of the spatial and temporal organization of gene expression. Although the spatial organization and dynamics of RNA polymerase II (RNAPII) transcription machinery have fundamental functional consequences for gene expression, its detailed studies have been abrogated by the limits of classical light microscopy for a long time. The advent of super-resolution microscopy (SRM) techniques allowed for the visualization of the RNAPII transcription machinery with nanometer resolution and millisecond precision. In this review, we summarize the recent methodological advances in SRM, focus on its application for studies of the nanoscale organization in space and time of RNAPII transcription, and discuss its consequences for the mechanistic understanding of gene expression.

650    _2

$a zvířata $7 D000818

650    12

$a regulace genové exprese $7 D005786

650    _2

$a lidé $7 D006801

650    12

$a fluorescenční mikroskopie $x metody $7 D008856

650    _2

$a vazba proteinů $7 D011485

650    _2

$a RNA-polymerasa II $x metabolismus $7 D012319

650    _2

$a zobrazení jednotlivé molekuly $x metody $7 D000072760

650    _2

$a transkripční faktory $x metabolismus $7 D014157

650    12

$a genetická transkripce $7 D014158

655    _2

$a časopisecké články $7 D016428

655    _2

$a přehledy $7 D016454

700    1_

$a Šebesta, Ondřej $u Faculty of Science, Charles University, Albertov 6, 128 00 Prague, Czech Republic

700    1_

$a Hozák, Pavel $u Department of Biology of the Cell Nucleus, Institute of Molecular Genetics of the Czech Academy of Sciences, Vídeňská 1083, 142 20 Prague, Czech Republic $u Microscopy Centre, Institute of Molecular Genetics of the Czech Academy of Sciences, Vídeňská 1083, 142 20 Prague, Czech Republic

773    0_

$w MED00176142 $t International journal of molecular sciences $x 1422-0067 $g Roč. 22, č. 13 (2021)

856    41

$u https://pubmed.ncbi.nlm.nih.gov/34206594 $y Pubmed

910    __

$a ABA008 $b sig $c sign $y p $z 0

990    __

$a 20211013 $b ABA008

991    __

$a 20211026133728 $b ABA008

999    __

$a ok $b bmc $g 1714551 $s 1146051

BAS    __

$a 3

BAS    __

$a PreBMC

BMC    __

$a 2021 $b 22 $c 13 $e 20210622 $i 1422-0067 $m International journal of molecular sciences $n Int J Mol Sci $x MED00176142

GRA    __

$a LM2018129 $p Ministry of Education, Youth and Science

GRA    __

$a CZ.02.1.01/0.0/0.0/18_046/0016045 $p Ministry of Education, Youth and Science

GRA    __

$a CZ.02.1.01/0.0/0.0/16_013/0001775 $p European Regional Development Fund

GRA    __

$a CZ.1.05/4.1.00/16.0347 $p European Regional Development Fund

GRA    __

$a CZ.2.16/3.1.00/21515 $p European Regional Development Fund

GRA    __

$a NPUI LO1220 and LO1419 (RVO: 68378050-KAV-NPUI) $p National Program of Sustainability

GRA    __

$a 19-05608S $p Grantová Agentura České Republiky

GRA    __

$a 18-19714S $p Grantová Agentura České Republiky

GRA    __

$a JSPS-20-06 $p Akademie Věd České Republiky

GRA    __

$a RVO: 68378050 $p Institutional Research Concept of the Institute of Molecular Genetics

GRA    __

$a COST Inter-excellence internship LTC19048 $p Ministry of Education, Youth and Science

GRA    __

$a LTC20024 $p Ministry of Education, Youth and Science

GRA    __

$a Action 15214 EuroCellnet $p Ministry of Education, Youth and Science

LZP    __

$a Pubmed-20211013