Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Wild boar as a potential reservoir of zoonotic tick-borne pathogens

K. Hrazdilová, PM. Lesiczka, J. Bardoň, Š. Vyroubalová, B. Šimek, L. Zurek, D. Modrý

. 2021 ; 12 (1) : 101558. [pub] 20200925

Jazyk angličtina Země Nizozemsko

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc21026315

The wild boar (Sus scrofa) population has increased dramatically over the last decades throughout Europe and it has become a serious pest. In addition, the common habitat of wild boar and of the tick, Ixodes ricinus, indicates the potential of wild boar to play a role in epidemiology of epizootic and zoonotic tick-borne pathogens, including Anaplasma phagocytophilum. In Europe, epidemiological cycles and reservoirs of A. phagocytophilum, including its zoonotic haplotypes, are poorly understood. In this study, we focused on detection and further genetic characterization of A. phagocytophilum and piroplasmids in 550 wild boars from eleven districts of Moravia and Silesia in the Czech Republic. Using highly sensitive nested PCR targeting the groEL gene, the DNA of A. phagocytophilum was detected in 28 wild boars (5.1 %) representing six unique haplotypes. The dominant haplotype was found in 21 samples from 7 different districts. All detected haplotypes clustered in the largest clade representing the European ecotype I and the dominant haplotype fell to the subclade with the European human cases and strains from dogs and horses. Nested PCR targeting the variable region of the 18S rRNA gene of piroplasmids resulted in one positive sample with 99.8 % sequence identity to Babesia divergens. The presence of these two pathogens that are primarily circulated by I. ricinus confirms the local participation of wild boar in the host spectrum of this tick and warrants experimental studies to address wild boar as a reservoir of zoonotic haplotypes of A. phagocytophilum.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc21026315
003      
CZ-PrNML
005      
20211026133011.0
007      
ta
008      
211013s2021 ne f 000 0|eng||
009      
AR
024    7_
$a 10.1016/j.ttbdis.2020.101558 $2 doi
035    __
$a (PubMed)33010631
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a ne
100    1_
$a Hrazdilová, Kristýna $u CEITEC - Central European Institute of Technology, University of Veterinary and Pharmaceutical Sciences Brno, Palackého třída 1946/1, Brno, 612 42, Czech Republic; Faculty of Medicine in Pilsen, Biomedical Center, Charles University, alej Svobody 1655/76, 32300, Plzeň, Czech Republic. Electronic address: kristyna@hrazdilova.cz
245    10
$a Wild boar as a potential reservoir of zoonotic tick-borne pathogens / $c K. Hrazdilová, PM. Lesiczka, J. Bardoň, Š. Vyroubalová, B. Šimek, L. Zurek, D. Modrý
520    9_
$a The wild boar (Sus scrofa) population has increased dramatically over the last decades throughout Europe and it has become a serious pest. In addition, the common habitat of wild boar and of the tick, Ixodes ricinus, indicates the potential of wild boar to play a role in epidemiology of epizootic and zoonotic tick-borne pathogens, including Anaplasma phagocytophilum. In Europe, epidemiological cycles and reservoirs of A. phagocytophilum, including its zoonotic haplotypes, are poorly understood. In this study, we focused on detection and further genetic characterization of A. phagocytophilum and piroplasmids in 550 wild boars from eleven districts of Moravia and Silesia in the Czech Republic. Using highly sensitive nested PCR targeting the groEL gene, the DNA of A. phagocytophilum was detected in 28 wild boars (5.1 %) representing six unique haplotypes. The dominant haplotype was found in 21 samples from 7 different districts. All detected haplotypes clustered in the largest clade representing the European ecotype I and the dominant haplotype fell to the subclade with the European human cases and strains from dogs and horses. Nested PCR targeting the variable region of the 18S rRNA gene of piroplasmids resulted in one positive sample with 99.8 % sequence identity to Babesia divergens. The presence of these two pathogens that are primarily circulated by I. ricinus confirms the local participation of wild boar in the host spectrum of this tick and warrants experimental studies to address wild boar as a reservoir of zoonotic haplotypes of A. phagocytophilum.
650    _2
$a Anaplasma phagocytophilum $x genetika $x izolace a purifikace $7 D041081
650    _2
$a anaplasmóza $x epidemiologie $x mikrobiologie $7 D000712
650    _2
$a zvířata $7 D000818
650    _2
$a babezióza $x epidemiologie $x parazitologie $7 D001404
650    _2
$a zdroje nemoci $x parazitologie $x veterinární $7 D004197
650    _2
$a bakteriální geny $7 D005798
650    _2
$a protozoální geny $7 D017125
650    12
$a genetická variace $7 D014644
650    _2
$a Piroplasmida $x genetika $x izolace a purifikace $7 D016792
650    _2
$a prevalence $7 D015995
650    _2
$a Sus scrofa $7 D034421
650    _2
$a prasata $7 D013552
650    _2
$a nemoci prasat $x epidemiologie $x mikrobiologie $x parazitologie $7 D013553
651    _2
$a Česká republika $x epidemiologie $7 D018153
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Lesiczka, Paulina Maria $u CEITEC - Central European Institute of Technology, University of Veterinary and Pharmaceutical Sciences Brno, Palackého třída 1946/1, Brno, 612 42, Czech Republic
700    1_
$a Bardoň, Jan $u State Veterinary Institute Olomouc, Jakoubka ze Stříbra No. 1, 779 00, Olomouc, Czech Republic; Department of Microbiology, Faculty of Medicine and Dentistry, Palacký University in Olomouc, Hněvotínská 976/3, 779 00, Olomouc, Czech Republic
700    1_
$a Vyroubalová, Šárka $u State Veterinary Institute Olomouc, Jakoubka ze Stříbra No. 1, 779 00, Olomouc, Czech Republic
700    1_
$a Šimek, Bronislav $u State Veterinary Institute Jihlava, Rantířovská 93/20, Horní Kosov, 58601, Jihlava, Czech Republic
700    1_
$a Zurek, Ludek $u CEITEC - Central European Institute of Technology, University of Veterinary and Pharmaceutical Sciences Brno, Palackého třída 1946/1, Brno, 612 42, Czech Republic; Department of Chemistry and Biochemistry, Mendel University, Zemědělská 1, Brno, Czech Republic
700    1_
$a Modrý, David $u CEITEC - Central European Institute of Technology, University of Veterinary and Pharmaceutical Sciences Brno, Palackého třída 1946/1, Brno, 612 42, Czech Republic; Department of Pathology and Parasitology, University of Veterinary and Pharmaceutical Sciences, Palackého 1946/1, 612 42, Brno, Czech Republic; Biology Centre, Institute of Parasitology, Czech Academy of Sciences, Branišovská 31, 37005, České Budějovice, Czech Republic; Department of Botany and Zoology, Faculty of Science, Masaryk University, Kotlářská 2, 611 37, Brno, Czech Republic
773    0_
$w MED00167597 $t Ticks and tick-borne diseases $x 1877-9603 $g Roč. 12, č. 1 (2021), s. 101558
856    41
$u https://pubmed.ncbi.nlm.nih.gov/33010631 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20211013 $b ABA008
991    __
$a 20211026133017 $b ABA008
999    __
$a ok $b bmc $g 1715131 $s 1146822
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2021 $b 12 $c 1 $d 101558 $e 20200925 $i 1877-9603 $m Ticks and tick-borne diseases $n Ticks Tick Borne Dis $x MED00167597
LZP    __
$a Pubmed-20211013

Najít záznam

Citační ukazatele

Pouze přihlášení uživatelé

Možnosti archivace