-
Je něco špatně v tomto záznamu ?
UHPLC coupled with charged aerosol detector for rapid separation of steviol glycosides in commercial sweeteners and extract of Stevia rebaudiana
M. Hollá, D. Šatínský, F. Švec, H. Sklenářová
Jazyk angličtina Země Velká Británie
Typ dokumentu časopisecké články
Odkazy
PubMed
34626939
DOI
10.1016/j.jpba.2021.114398
Knihovny.cz E-zdroje
- MeSH
- aerosoly MeSH
- diterpeny kauranové * analýza MeSH
- glukosidy MeSH
- glykosidy MeSH
- lidé MeSH
- listy rostlin chemie MeSH
- rostlinné extrakty MeSH
- sladidla analýza MeSH
- Stevia * MeSH
- vysokoúčinná kapalinová chromatografie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
Natural sweeteners are in high demand as a part of a healthy lifestyle. Among them, sweeteners with decreased caloric value and suitability for diabetes patients are most requested. Extension in their consumption extends the need for their quality control. A fast gradient UHPLC coupled with charged aerosol detection enabling quantitation of stevioside, rebaudioside A-D, and steviolbioside in commercial sweeteners and Stevia rebaudiana plant extracts has been developed. The method was developed to achieve high efficiency, simplicity, versatility, and low solvent consumption. All steviol glycosides were baseline-separated in less than 4 min with a total run time of 7 min. Buffer-free eluents were used in the separations and only 2.45 mL solvent were needed per analysis. The Luna Omega Polar column featuring polar modification of the C18 stationary phase was employed with mobile phases composed of water and acetonitrile for the excellent separation of polar steviol glycosides. The flow rate of the mobile phase 0.35 mL/min, column temperature 50 °C and injection volume 2 µL were used. Critical pair of glycosides, stevioside and rebaudioside A, were baseline separated with a resolution of 2.41. The universal charged aerosol detector allowed quantitation of steviol glycosides with a limit of detection and quantitation 0.15 and 0.5 µg/mL, respectively. Method intra-day precision was less than 2% (RSD), and the recovery was 89.6-105.0% and 93.8-111.4% for plant material and sweetener tablets, respectively. The quantity of steviol glycosides in three out of four commercial sweeteners was 3.0-12.3% higher than declared. The content was about 12.4% less than declared in one sample. But the difference from the labeled content corresponded to trueness and precision of the developed method together with variability of sweeteners production. The most abundant glycoside detected in sweeteners was stevioside followed by rebaudioside A. A leaf-to-stem ratio describing the dominant accumulation of steviol glycosides in leaves affected the differences in the amount of steviol glycosides among plant samples.
- 000
- 00000naa a2200000 a 4500
- 001
- bmc22002971
- 003
- CZ-PrNML
- 005
- 20220127150748.0
- 007
- ta
- 008
- 220113s2022 xxk f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1016/j.jpba.2021.114398 $2 doi
- 035 __
- $a (PubMed)34626939
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxk
- 100 1_
- $a Hollá, Marcela $u Department of Analytical Chemistry, Faculty of Pharmacy in Hradec Králové, Charles University, 50005 Hradec Králové, Czech Republic
- 245 10
- $a UHPLC coupled with charged aerosol detector for rapid separation of steviol glycosides in commercial sweeteners and extract of Stevia rebaudiana / $c M. Hollá, D. Šatínský, F. Švec, H. Sklenářová
- 520 9_
- $a Natural sweeteners are in high demand as a part of a healthy lifestyle. Among them, sweeteners with decreased caloric value and suitability for diabetes patients are most requested. Extension in their consumption extends the need for their quality control. A fast gradient UHPLC coupled with charged aerosol detection enabling quantitation of stevioside, rebaudioside A-D, and steviolbioside in commercial sweeteners and Stevia rebaudiana plant extracts has been developed. The method was developed to achieve high efficiency, simplicity, versatility, and low solvent consumption. All steviol glycosides were baseline-separated in less than 4 min with a total run time of 7 min. Buffer-free eluents were used in the separations and only 2.45 mL solvent were needed per analysis. The Luna Omega Polar column featuring polar modification of the C18 stationary phase was employed with mobile phases composed of water and acetonitrile for the excellent separation of polar steviol glycosides. The flow rate of the mobile phase 0.35 mL/min, column temperature 50 °C and injection volume 2 µL were used. Critical pair of glycosides, stevioside and rebaudioside A, were baseline separated with a resolution of 2.41. The universal charged aerosol detector allowed quantitation of steviol glycosides with a limit of detection and quantitation 0.15 and 0.5 µg/mL, respectively. Method intra-day precision was less than 2% (RSD), and the recovery was 89.6-105.0% and 93.8-111.4% for plant material and sweetener tablets, respectively. The quantity of steviol glycosides in three out of four commercial sweeteners was 3.0-12.3% higher than declared. The content was about 12.4% less than declared in one sample. But the difference from the labeled content corresponded to trueness and precision of the developed method together with variability of sweeteners production. The most abundant glycoside detected in sweeteners was stevioside followed by rebaudioside A. A leaf-to-stem ratio describing the dominant accumulation of steviol glycosides in leaves affected the differences in the amount of steviol glycosides among plant samples.
- 650 _2
- $a aerosoly $7 D000336
- 650 _2
- $a vysokoúčinná kapalinová chromatografie $7 D002851
- 650 12
- $a diterpeny kauranové $x analýza $7 D045786
- 650 _2
- $a glukosidy $7 D005960
- 650 _2
- $a glykosidy $7 D006027
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a rostlinné extrakty $7 D010936
- 650 _2
- $a listy rostlin $x chemie $7 D018515
- 650 12
- $a Stevia $7 D036625
- 650 _2
- $a sladidla $x analýza $7 D013549
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Šatínský, Dalibor $u Department of Analytical Chemistry, Faculty of Pharmacy in Hradec Králové, Charles University, 50005 Hradec Králové, Czech Republic
- 700 1_
- $a Švec, František $u Department of Analytical Chemistry, Faculty of Pharmacy in Hradec Králové, Charles University, 50005 Hradec Králové, Czech Republic
- 700 1_
- $a Sklenářová, Hana $u Department of Analytical Chemistry, Faculty of Pharmacy in Hradec Králové, Charles University, 50005 Hradec Králové, Czech Republic. Electronic address: sklenarova@faf.cuni.cz
- 773 0_
- $w MED00002894 $t Journal of pharmaceutical and biomedical analysis $x 1873-264X $g Roč. 207, č. - (2022), s. 114398
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/34626939 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y p $z 0
- 990 __
- $a 20220113 $b ABA008
- 991 __
- $a 20220127150745 $b ABA008
- 999 __
- $a ok $b bmc $g 1750685 $s 1154120
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2022 $b 207 $c - $d 114398 $e 20210930 $i 1873-264X $m Journal of pharmaceutical and biomedical analysis $n J Pharm Biomed Anal $x MED00002894
- LZP __
- $a Pubmed-20220113