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Strengths and Weaknesses of Cell Synchronization Protocols Based on Inhibition of DNA Synthesis
A. Ligasová, K. Koberna
Jazyk angličtina Země Švýcarsko
Typ dokumentu časopisecké články, přehledy
Grantová podpora
TN01000013
Technology Agency of the Czech Republic
project EATRIS-CZ, grant number LM2015064
Ministry of Education Youth and Sports
project ENOCH, grant number CZ.02.1.01/0.0/0.0/16_019/0000868
European Regional Development Fund
NLK
Free Medical Journals
od 2000
Freely Accessible Science Journals
od 2000
PubMed Central
od 2007
Europe PubMed Central
od 2007
ProQuest Central
od 2000-03-01
Open Access Digital Library
od 2000-01-01
Open Access Digital Library
od 2007-01-01
Health & Medicine (ProQuest)
od 2000-03-01
ROAD: Directory of Open Access Scholarly Resources
od 2000
PubMed
34639098
DOI
10.3390/ijms221910759
Knihovny.cz E-zdroje
- MeSH
- buněčné dělení * MeSH
- buněčný cyklus * MeSH
- DNA antagonisté a inhibitory biosyntéza MeSH
- lidé MeSH
- replikace DNA * MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
Synchronous cell populations are commonly used for the analysis of various aspects of cellular metabolism at specific stages of the cell cycle. Cell synchronization at a chosen cell cycle stage is most frequently achieved by inhibition of specific metabolic pathway(s). In this respect, various protocols have been developed to synchronize cells in particular cell cycle stages. In this review, we provide an overview of the protocols for cell synchronization of mammalian cells based on the inhibition of synthesis of DNA building blocks-deoxynucleotides and/or inhibition of DNA synthesis. The mechanism of action, examples of their use, and advantages and disadvantages are described with the aim of providing a guide for the selection of suitable protocol for different studied situations.
Citace poskytuje Crossref.org
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