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Development of CE-C4D Method for Determination Tropane Alkaloids

M. Gołąb, M. Przybyłowska, P. Kubáň, P. Itterheimová, M. Woźniakiewicz

. 2021 ; 26 (19) : . [pub] 20210922

Language English Country Switzerland

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc22003690

Grant support
2016/23/B/ST4/00789 Narodowym Centrum Nauki
68081715 RVO CEP Register
CA16215 COST Action

A fast method for the determination of tropane alkaloids, using a portable CE instrument with a capacitively coupled contactless conductivity detector (CE-C4D) was developed and validated for determination of atropine and scopolamine in seeds from Solanaceae family plants. Separation was obtained within 5 min, using an optimized background electrolyte consisting of 0.5 M acetic acid with 0.25% (w/v) β-CD. The limit of detection and quantification was 0.5 µg/mL and 1.5 µg/mL, respectively, for both atropine and scopolamine. The developed method was validated with the following parameters-precision (CV): 1.07-2.08%, accuracy of the assay (recovery, RE): 101.0-102.7% and matrix effect (ME): 92.99-94.23%. Moreover, the optimized CE-C4D method was applied to the analysis of plant extracts and pharmaceuticals, proving its applicability and accuracy.

References provided by Crossref.org

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