-
Something wrong with this record ?
Web-based tools for computational enzyme design
SM. Marques, J. Planas-Iglesias, J. Damborsky
Language English Country Great Britain
Document type Journal Article, Research Support, Non-U.S. Gov't, Review
- MeSH
- Biotechnology * MeSH
- Internet * MeSH
- Solubility MeSH
- Computational Biology MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Review MeSH
Enzymes are in high demand for very diverse biotechnological applications. However, natural biocatalysts often need to be engineered for fine-tuning their properties towards the end applications, such as the activity, selectivity, stability to temperature or co-solvents, and solubility. Computational methods are increasingly used in this task, providing predictions that narrow down the space of possible mutations significantly and can enormously reduce the experimental burden. Many computational tools are available as web-based platforms, making them accessible to non-expert users. These platforms are typically user-friendly, contain walk-throughs, and do not require deep expertise and installations. Here we describe some of the most recent outstanding web-tools for enzyme engineering and formulate future perspectives in this field.
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc22003987
- 003
- CZ-PrNML
- 005
- 20220127145648.0
- 007
- ta
- 008
- 220113s2021 xxk f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1016/j.sbi.2021.01.010 $2 doi
- 035 __
- $a (PubMed)33667757
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxk
- 100 1_
- $a Marques, Sérgio M $u Loschmidt Laboratories, Department of Experimental Biology and RECETOX, Faculty of Science, Masaryk University, Kamenice 5/C13, 625 00 Brno, Czech Republic; International Centre for Clinical Research, St. Anne's University Hospital Brno, Pekarska 53, 656 91 Brno, Czech Republic
- 245 10
- $a Web-based tools for computational enzyme design / $c SM. Marques, J. Planas-Iglesias, J. Damborsky
- 520 9_
- $a Enzymes are in high demand for very diverse biotechnological applications. However, natural biocatalysts often need to be engineered for fine-tuning their properties towards the end applications, such as the activity, selectivity, stability to temperature or co-solvents, and solubility. Computational methods are increasingly used in this task, providing predictions that narrow down the space of possible mutations significantly and can enormously reduce the experimental burden. Many computational tools are available as web-based platforms, making them accessible to non-expert users. These platforms are typically user-friendly, contain walk-throughs, and do not require deep expertise and installations. Here we describe some of the most recent outstanding web-tools for enzyme engineering and formulate future perspectives in this field.
- 650 12
- $a biotechnologie $7 D001709
- 650 _2
- $a výpočetní biologie $7 D019295
- 650 12
- $a internet $7 D020407
- 650 _2
- $a rozpustnost $7 D012995
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 655 _2
- $a přehledy $7 D016454
- 700 1_
- $a Planas-Iglesias, Joan $u Loschmidt Laboratories, Department of Experimental Biology and RECETOX, Faculty of Science, Masaryk University, Kamenice 5/C13, 625 00 Brno, Czech Republic; International Centre for Clinical Research, St. Anne's University Hospital Brno, Pekarska 53, 656 91 Brno, Czech Republic
- 700 1_
- $a Damborsky, Jiri $u Loschmidt Laboratories, Department of Experimental Biology and RECETOX, Faculty of Science, Masaryk University, Kamenice 5/C13, 625 00 Brno, Czech Republic; International Centre for Clinical Research, St. Anne's University Hospital Brno, Pekarska 53, 656 91 Brno, Czech Republic. Electronic address: jiri@chemi.muni.cz
- 773 0_
- $w MED00006632 $t Current opinion in structural biology $x 1879-033X $g Roč. 69, č. - (2021), s. 19-34
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/33667757 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y p $z 0
- 990 __
- $a 20220113 $b ABA008
- 991 __
- $a 20220127145644 $b ABA008
- 999 __
- $a ok $b bmc $g 1751447 $s 1155136
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2021 $b 69 $c - $d 19-34 $e 20210302 $i 1879-033X $m Current opinion in structural biology $n Curr Opin Struct Biol $x MED00006632
- LZP __
- $a Pubmed-20220113
