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Patients With Common Variable Immunodeficiency (CVID) Show Higher Gut Bacterial Diversity and Levels of Low-Abundance Genes Than the Healthy Housemates
J. Bosák, M. Lexa, K. Fiedorová, DC. Gadara, L. Micenková, Z. Spacil, J. Litzman, T. Freiberger, D. Šmajs
Jazyk angličtina Země Švýcarsko
Typ dokumentu časopisecké články, práce podpořená grantem
NLK
Directory of Open Access Journals
od 2010
Free Medical Journals
od 2010
PubMed Central
od 2010
Europe PubMed Central
od 2010
Open Access Digital Library
od 2010-01-01
Open Access Digital Library
od 2010-01-01
ROAD: Directory of Open Access Scholarly Resources
od 2010
- MeSH
- adenosin metabolismus MeSH
- běžná variabilní imunodeficience genetika mikrobiologie MeSH
- biodiverzita MeSH
- Clostridiaceae fyziologie MeSH
- dysbióza genetika mikrobiologie MeSH
- feces mikrobiologie MeSH
- homeostáza MeSH
- lidé MeSH
- metabolomika MeSH
- metagenom MeSH
- RNA ribozomální 16S genetika MeSH
- střevní mikroflóra genetika MeSH
- výpočetní biologie metody MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Common variable immunodeficiency (CVID) is a clinically and genetically heterogeneous disorder with inadequate antibody responses and low levels of immunoglobulins including IgA that is involved in the maintenance of the intestinal homeostasis. In this study, we analyzed the taxonomical and functional metagenome of the fecal microbiota and stool metabolome in a cohort of six CVID patients without gastroenterological symptomatology and their healthy housemates. The fecal microbiome of CVID patients contained higher numbers of bacterial species and altered abundance of thirty-four species. Hungatella hathewayi was frequent in CVID microbiome and absent in controls. Moreover, the CVID metagenome was enriched for low-abundance genes likely encoding nonessential functions, such as bacterial motility and metabolism of aromatic compounds. Metabolomics revealed dysregulation in several metabolic pathways, mostly associated with decreased levels of adenosine in CVID patients. Identified features have been consistently associated with CVID diagnosis across the patients with various immunological characteristics, length of treatment, and age. Taken together, this initial study revealed expansion of bacterial diversity in the host immunodeficient conditions and suggested several bacterial species and metabolites, which have potential to be diagnostic and/or prognostic CVID markers in the future.
Centre for Cardiovascular Surgery and Transplantation Brno Czechia
Department of Biology Faculty of Medicine Masaryk University Brno Czechia
Department of Clinical Immunology and Allergology St Anne's University Hospital in Brno Brno Czechia
Faculty of Informatics Masaryk University Brno Czechia
RECETOX Center Faculty of Science Masaryk University Brno Czechia
Citace poskytuje Crossref.org
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