-
Je něco špatně v tomto záznamu ?
Oligopeptide-targeted polymer nanoprobes for fluorescence-guided endoscopic surgery
R Pola, J Parnica, K Zuska, E Böhmová, M Filipová, M Pechar, J Pankrác, J Mucksová, J Kalina, P Trefil, L Šefc, D Větvička, P Poučková, J Bouček, O Janoušková and T Etrych
Jazyk angličtina Země Velká Británie
Typ dokumentu práce podpořená grantem
Grantová podpora
NV16-28594A
MZ0
CEP - Centrální evidence projektů
- MeSH
- endoskopie * metody MeSH
- fluorescence MeSH
- fluorescenční barviva MeSH
- lidé MeSH
- nádorové biomarkery MeSH
- nádory chirurgie diagnostické zobrazování MeSH
- nanočástice MeSH
- oligopeptidy MeSH
- polymery MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
A polymer probe based on N-(2-hydroxypropyl)methacrylamide copolymers labelled with a fluorescent dye Dy-633 or Cy-7 and decorated with targeting oligopeptides GE-7 or GE-11, specific targeting ligands binding to epidermal growth factor receptor (EGFR) highly expressed on surface of tumour cells, was designed, synthesised and characterised. Specific accumulation of the polymer probe in the tumour mass is a prerequisite for successful fluorescence-guided endoscopic surgery as the fluorescence signal from the malignant cells enables more precise resection of the tumour without damaging the healthy tissue. Flow cytometry and confocal microscopy was used to assess the binding efficacy of the oligopeptide conjugates to EGFR on the cell membranes of the malignant cells. The results showed that the highest binding efficacy was achieved with polymers bearing the GE-11 targeting oligopeptide in human EGFR-positive hypopharyngeal carcinoma cells (FaDu) and in breast adenocarcinoma cells (MDA-MB-231). Similarly, the polymer probes targeted by the GE-11 oligopeptidewere found in vivo as highly effective in tumour accumulation, as determined from fluorescence imaging. Indeed, the ex vivo cross-section of the tumours showed significant tumour border fluorescence proving the potential of the studied polymer probes. Moreover, the presence of the active targeting moiety on the polymer-drug conjugate should enable the use of such a conjugate as a targeted polymer system for treatment of solid tumours. Replacement of the fluorescent probe with a cytostatic drug provides a targeted polymer nanocancerostatic for advanced treatment of neoplastic diseases, thus the polymer probes have multiple functions.
1st Faculty of Medicine Charles University Prague U Nemocnice 5 120 00 Prague 2 Czechia
BIOPHARM Research Institute of Biopharmacy and Veterinary Drugs a s 254 49 Jilove u Prahy Czechia
- 000
- 00000naa a2200000 a 4500
- 001
- bmc22008065
- 003
- CZ-PrNML
- 005
- 20240624085150.0
- 007
- ta
- 008
- 220311s2019 xxk f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1088/2399-7532/ab159e $2 doi
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxk
- 100 1_
- $a Pola, Robert $7 xx0232782 $u Institute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovského sq. 2, 162 06 Prague 6, Czechia (www.imc.cas.cz)
- 245 10
- $a Oligopeptide-targeted polymer nanoprobes for fluorescence-guided endoscopic surgery / $c R Pola, J Parnica, K Zuska, E Böhmová, M Filipová, M Pechar, J Pankrác, J Mucksová, J Kalina, P Trefil, L Šefc, D Větvička, P Poučková, J Bouček, O Janoušková and T Etrych
- 520 9_
- $a A polymer probe based on N-(2-hydroxypropyl)methacrylamide copolymers labelled with a fluorescent dye Dy-633 or Cy-7 and decorated with targeting oligopeptides GE-7 or GE-11, specific targeting ligands binding to epidermal growth factor receptor (EGFR) highly expressed on surface of tumour cells, was designed, synthesised and characterised. Specific accumulation of the polymer probe in the tumour mass is a prerequisite for successful fluorescence-guided endoscopic surgery as the fluorescence signal from the malignant cells enables more precise resection of the tumour without damaging the healthy tissue. Flow cytometry and confocal microscopy was used to assess the binding efficacy of the oligopeptide conjugates to EGFR on the cell membranes of the malignant cells. The results showed that the highest binding efficacy was achieved with polymers bearing the GE-11 targeting oligopeptide in human EGFR-positive hypopharyngeal carcinoma cells (FaDu) and in breast adenocarcinoma cells (MDA-MB-231). Similarly, the polymer probes targeted by the GE-11 oligopeptidewere found in vivo as highly effective in tumour accumulation, as determined from fluorescence imaging. Indeed, the ex vivo cross-section of the tumours showed significant tumour border fluorescence proving the potential of the studied polymer probes. Moreover, the presence of the active targeting moiety on the polymer-drug conjugate should enable the use of such a conjugate as a targeted polymer system for treatment of solid tumours. Replacement of the fluorescent probe with a cytostatic drug provides a targeted polymer nanocancerostatic for advanced treatment of neoplastic diseases, thus the polymer probes have multiple functions.
- 650 _7
- $a fluorescenční barviva $7 D005456 $2 czmesh
- 650 _7
- $a fluorescence $7 D005453 $2 czmesh
- 650 17
- $a endoskopie $x metody $7 D004724 $2 czmesh
- 650 _7
- $a nanočástice $7 D053758 $2 czmesh
- 650 _7
- $a polymery $7 D011108 $2 czmesh
- 650 _7
- $a nádorové biomarkery $7 D014408 $2 czmesh
- 650 _7
- $a nádory $x chirurgie $x diagnostické zobrazování $7 D009369 $2 czmesh
- 650 _7
- $a oligopeptidy $7 D009842 $2 czmesh
- 650 _7
- $a lidé $7 D006801 $2 czmesh
- 655 _7
- $a práce podpořená grantem $7 D013485 $2 czmesh
- 700 1_
- $a Parnica, J $u Institute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovského sq. 2, 162 06 Prague 6, Czechia (www.imc.cas.cz)
- 700 1_
- $a Zuska, K $u Institute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovského sq. 2, 162 06 Prague 6, Czechia (www.imc.cas.cz)
- 700 1_
- $a Böhmová, Eliška $u Institute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovského sq. 2, 162 06 Prague 6, Czechia (www.imc.cas.cz) $7 xx0232781
- 700 1_
- $a Filipová, M $u Institute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovského sq. 2, 162 06 Prague 6, Czechia (www.imc.cas.cz)
- 700 1_
- $a Pechar, Michal, $d 1967- $u First Faculty of Medicine, Charles University Prague, U Nemocnice 5, 120 00, Prague 2, Czechia $7 xx0141621
- 700 1_
- $a Pankrác, J $u First Faculty of Medicine, Charles University Prague, U Nemocnice 5, 120 00, Prague 2, Czechia
- 700 1_
- $a Mucksová, J $u BIOPHARM, Research Institute of Biopharmacy and Veterinary Drugs, a.s., 254 49 Jilove u Prahy, Czechia
- 700 1_
- $a Kalina, J $u BIOPHARM, Research Institute of Biopharmacy and Veterinary Drugs, a.s., 254 49 Jilove u Prahy, Czechia
- 700 1_
- $a Trefil, P $u BIOPHARM, Research Institute of Biopharmacy and Veterinary Drugs, a.s., 254 49 Jilove u Prahy, Czechia
- 700 1_
- $a Šefc, Luděk, $d 1960- $u First Faculty of Medicine, Charles University Prague, U Nemocnice 5, 120 00, Prague 2, Czechia $7 xx0092587
- 700 1_
- $a Větvička, David $u Institute of Biophysics and Informatics, First Faculty of Medicine, Charles University, Salmovská 1, 120 00, Prague 2, Czechia $7 xx0214483
- 700 1_
- $a Poučková, Pavla, $d 1950- $u Institute of Biophysics and Informatics, First Faculty of Medicine, Charles University, Salmovská 1, 120 00, Prague 2, Czechia $7 jo2002102951
- 700 1_
- $a Bouček, Jan, $d 1976- $u Department of Otorhinolaryngology Head and Neck Surgery, First Faculty of Medicine, Charles University and University Hospital Motol, V Úvalu 84, 150 06, Prague 5, Czechia $7 xx0100852
- 700 1_
- $a Janoušková, O $u Institute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovského sq. 2, 162 06 Prague 6, Czechia (www.imc.cas.cz)
- 700 1_
- $a Etrych, T $u Institute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovského sq. 2, 162 06 Prague 6, Czechia (www.imc.cas.cz)
- 773 0_
- $t Multifunctional Materials $x 2399-7532 $g Roč. 2, č. 2 (2019), s. 024004 $w boh
- 910 __
- $a ABA008 $y 0 $z 0
- 990 __
- $a 20220311180810 $b ABA008
- 991 __
- $a 20240624085148 $b ABA008
- 999 __
- $a kom $b bmc $g 1769015 $s 1159259
- BAS __
- $a 3
- BMC __
- $a 2019 $b 2 $c 2 $d 024004 $x boh $i 2399-7532 $m Multifunctional Materials
- GRA __
- $a NV16-28594A $p MZ0
- LZP __
- $c NLK120 $d 20240621