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The impact of extractable organic matter from gasoline and alternative fuel emissions on bronchial cell models (BEAS-2B, MucilAirTM)

M. Sima, T. Cervena, F. Elzeinova, A. Ambroz, V. Beranek, M. Vojtisek-Lom, J. Klema, M. Ciganek, P. Rossner

. 2022 ; 80 (-) : 105316. [pub] 20220121

Jazyk angličtina Země Velká Británie

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc22010734

Air pollution caused by road traffic has an unfavorable impact on the environment and also on human health. It has previously been shown, that complete gasoline emissions lead to toxic effects in cell models originating from human airways. Here we focused on extractable organic matter (EOM) from particulate matter, collected from gasoline emissions from fuels with different ethanol content. We performed cytotoxicity evaluation, quantification of mucin and extracellular reactive oxygen species (ROS) production, DNA breaks detection, and selected gene deregulation analysis, after one and five days of exposure of human bronchial epithelial model (BEAS-2B) and a 3D model of the human airway (MucilAirTM). Our data suggest that the longer exposure had more pronounced effects on the parameters of cytotoxicity and mucin production, while the impacts on ROS generation and DNA integrity were limited. In both cell models the expression of CYP1A1 was induced, regardless of the exposure period or EOM tested. Several other genes, including FMO2, IL1A, or TNF, were deregulated depending on the exposure time. In conclusion, ethanol content in the fuels did not significantly impact the toxicity of EOM. Biological effects were mostly linked to xenobiotics metabolism and inflammatory response. BEAS-2B cells were more sensitive to the treatment.

Citace poskytuje Crossref.org

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$a Sima, Michal $u Department of Nanotoxicology and Molecular Epidemiology, Institute of Experimental Medicine CAS, Videnska 1083, 142 20 Prague, Czech Republic. Electronic address: michal.sima@iem.cas.cz
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$a Air pollution caused by road traffic has an unfavorable impact on the environment and also on human health. It has previously been shown, that complete gasoline emissions lead to toxic effects in cell models originating from human airways. Here we focused on extractable organic matter (EOM) from particulate matter, collected from gasoline emissions from fuels with different ethanol content. We performed cytotoxicity evaluation, quantification of mucin and extracellular reactive oxygen species (ROS) production, DNA breaks detection, and selected gene deregulation analysis, after one and five days of exposure of human bronchial epithelial model (BEAS-2B) and a 3D model of the human airway (MucilAirTM). Our data suggest that the longer exposure had more pronounced effects on the parameters of cytotoxicity and mucin production, while the impacts on ROS generation and DNA integrity were limited. In both cell models the expression of CYP1A1 was induced, regardless of the exposure period or EOM tested. Several other genes, including FMO2, IL1A, or TNF, were deregulated depending on the exposure time. In conclusion, ethanol content in the fuels did not significantly impact the toxicity of EOM. Biological effects were mostly linked to xenobiotics metabolism and inflammatory response. BEAS-2B cells were more sensitive to the treatment.
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$a Cervena, Tereza $u Department of Nanotoxicology and Molecular Epidemiology, Institute of Experimental Medicine CAS, Videnska 1083, 142 20 Prague, Czech Republic; Department of Physiology, Faculty of Science, Charles University, Vinicna 7, 128 44 Prague, Czech Republic. Electronic address: tereza.cervena@iem.cas.cz
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$a Elzeinova, Fatima $u Department of Nanotoxicology and Molecular Epidemiology, Institute of Experimental Medicine CAS, Videnska 1083, 142 20 Prague, Czech Republic. Electronic address: fatima.elzeinova@iem.cas.cz
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$a Ambroz, Antonin $u Department of Nanotoxicology and Molecular Epidemiology, Institute of Experimental Medicine CAS, Videnska 1083, 142 20 Prague, Czech Republic. Electronic address: antonin.ambroz@iem.cas.cz
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$a Beranek, Vit $u Centre of Vehicles for Sustainable Mobility, Faculty of Mechanical Engineering, Czech Technical University in Prague, Technicka 4, 160 00 Prague, Czech Republic. Electronic address: vit.beranek@fs.cvut.cz
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$a Vojtisek-Lom, Michal $u Centre of Vehicles for Sustainable Mobility, Faculty of Mechanical Engineering, Czech Technical University in Prague, Technicka 4, 160 00 Prague, Czech Republic. Electronic address: michal.vojtisek@fs.cvut.cz
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$a Klema, Jiri $u Department of Computer Science, Czech Technical University in Prague, 121 35 Prague, Czech Republic. Electronic address: klema@fel.cvut.cz
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$a Ciganek, Miroslav $u Department of Chemistry and Toxicology, Veterinary Research Institute, 621 00 Brno, Czech Republic. Electronic address: ciganek@vri.cz
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$a Rossner, Pavel $u Department of Nanotoxicology and Molecular Epidemiology, Institute of Experimental Medicine CAS, Videnska 1083, 142 20 Prague, Czech Republic. Electronic address: pavel.rossner@iem.cas.cz
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