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Comparison of proteomic approaches used for the detection of potential biomarkers of Alzheimer's disease in blood plasma
TA. Smirnova, A. Viskin, M. Hoskova, L. Habartova, V. Setnicka, P. Cejnar, S. Kuckova
Jazyk angličtina Země Německo
Typ dokumentu časopisecké články
Grantová podpora
A2_FPBT_2021_016
Specific University Research
A1_FPBT_2021_003
Specific University Research
A2_FCHI-2020_003
Specific University Research
17-05292S
Czech Science Foundation
LTAIN19007
Ministry of Education, Youth and Sports of the Czech Republic
Odkazy
PubMed
34545700
DOI
10.1002/jssc.202100468
Knihovny.cz E-zdroje
- MeSH
- Alzheimerova nemoc * krev diagnóza MeSH
- biologické markery krev MeSH
- chromatografie kapalinová metody MeSH
- krevní plazma chemie MeSH
- krevní proteiny analýza MeSH
- lidé středního věku MeSH
- lidé MeSH
- lipidy krev MeSH
- metabolismus lipidů MeSH
- proteomika metody MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- spektrometrie hmotnostní - ionizace laserem za účasti matrice metody MeSH
- tandemová hmotnostní spektrometrie metody MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
At present, Alzheimer's disease is detected mainly using psychological tests, which can only confirm the disease in its more advanced phases. Therefore, bioanalytical possibilities for detecting this disease earlier are being investigated. To date, the results of analyses, which focus mainly on the study of lipids and proteins either in cerebrospinal fluid or much less often in blood plasma, do not provide satisfactory results. In addition, cerebrospinal fluid sampling is uncomfortable for the patients and involves many health risks. In this work, we deal with proteomic analysis using Matrix-Assisted Laser Desorption/Ionisation-Time of Flight and Liquid Chromatography coupled to tandem Mass Spectrometry of blood plasma with a focus on various ways of preanalytical sample treatments. This should lead to results improvement and facilitate the subsequent evaluation using principal component analysis and partial least squares discriminant analysis. The obtained results indicate the direction of further research, namely the study of interactions between proteins and lipids contained in blood plasma. These substances may be regarded as potential biomarkers allowing for the diagnosis of Alzheimer ́s disease even in its early stages.
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- $a Smirnova, Tatiana Anatolievna $u Department of Biochemistry and Microbiology, University of Chemistry and Technology Prague, Prague, Czech Republic $1 https://orcid.org/0000000327130721
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- $a At present, Alzheimer's disease is detected mainly using psychological tests, which can only confirm the disease in its more advanced phases. Therefore, bioanalytical possibilities for detecting this disease earlier are being investigated. To date, the results of analyses, which focus mainly on the study of lipids and proteins either in cerebrospinal fluid or much less often in blood plasma, do not provide satisfactory results. In addition, cerebrospinal fluid sampling is uncomfortable for the patients and involves many health risks. In this work, we deal with proteomic analysis using Matrix-Assisted Laser Desorption/Ionisation-Time of Flight and Liquid Chromatography coupled to tandem Mass Spectrometry of blood plasma with a focus on various ways of preanalytical sample treatments. This should lead to results improvement and facilitate the subsequent evaluation using principal component analysis and partial least squares discriminant analysis. The obtained results indicate the direction of further research, namely the study of interactions between proteins and lipids contained in blood plasma. These substances may be regarded as potential biomarkers allowing for the diagnosis of Alzheimer ́s disease even in its early stages.
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