• Je něco špatně v tomto záznamu ?

Determination of Butyrate Synthesis Capacity in Gut Microbiota: Quantification of but Gene Abundance by qPCR in Fecal Samples

N. Daskova, M. Heczkova, I. Modos, P. Videnska, P. Splichalova, H. Pelantova, M. Kuzma, J. Gojda, M. Cahova

. 2021 ; 11 (9) : . [pub] 20210902

Jazyk angličtina Země Švýcarsko

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc22012227

Butyrate is formed in the gut during bacterial fermentation of dietary fiber and is attributed numerous beneficial effects on the host metabolism. We aimed to develop a method for the assessment of functional capacity of gut microbiota butyrate synthesis based on the qPCR quantification of bacterial gene coding butyryl-CoA:acetate CoA-transferase, the key enzyme of butyrate synthesis. In silico, we identified bacteria possessing but gene among human gut microbiota by searching but coding sequences in available databases. We designed and validated six sets of degenerate primers covering all selected bacteria, based on their phylogenetic nearness and sequence similarity, and developed a method for gene abundance normalization in human fecal DNA. We determined but gene abundance in fecal DNA of subjects with opposing dietary patterns and metabolic phenotypes-lean vegans (VG) and healthy obese omnivores (OB) with known fecal microbiota and metabolome composition. We found higher but gene copy number in VG compared with OB, in line with higher fecal butyrate content in VG group. We further found a positive correlation between the relative abundance of target bacterial genera identified by next-generation sequencing and groups of but gene-containing bacteria determined by specific primers. In conclusion, this approach represents a simple and feasible tool for estimation of microbial functional capacity.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc22012227
003      
CZ-PrNML
005      
20220506131320.0
007      
ta
008      
220425s2021 sz f 000 0|eng||
009      
AR
024    7_
$a 10.3390/biom11091303 $2 doi
035    __
$a (PubMed)34572516
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a sz
100    1_
$a Daskova, Nikola $u Institute for Clinical and Experimental Medicine, Videnska 1958, 140 21 Prague 4, Czech Republic $u First Faculty of Medicine, Charles University, Katerinska 1660/32, 121 08 Prague, Czech Republic $1 https://orcid.org/0000000280388361
245    10
$a Determination of Butyrate Synthesis Capacity in Gut Microbiota: Quantification of but Gene Abundance by qPCR in Fecal Samples / $c N. Daskova, M. Heczkova, I. Modos, P. Videnska, P. Splichalova, H. Pelantova, M. Kuzma, J. Gojda, M. Cahova
520    9_
$a Butyrate is formed in the gut during bacterial fermentation of dietary fiber and is attributed numerous beneficial effects on the host metabolism. We aimed to develop a method for the assessment of functional capacity of gut microbiota butyrate synthesis based on the qPCR quantification of bacterial gene coding butyryl-CoA:acetate CoA-transferase, the key enzyme of butyrate synthesis. In silico, we identified bacteria possessing but gene among human gut microbiota by searching but coding sequences in available databases. We designed and validated six sets of degenerate primers covering all selected bacteria, based on their phylogenetic nearness and sequence similarity, and developed a method for gene abundance normalization in human fecal DNA. We determined but gene abundance in fecal DNA of subjects with opposing dietary patterns and metabolic phenotypes-lean vegans (VG) and healthy obese omnivores (OB) with known fecal microbiota and metabolome composition. We found higher but gene copy number in VG compared with OB, in line with higher fecal butyrate content in VG group. We further found a positive correlation between the relative abundance of target bacterial genera identified by next-generation sequencing and groups of but gene-containing bacteria determined by specific primers. In conclusion, this approach represents a simple and feasible tool for estimation of microbial functional capacity.
650    _2
$a mladiství $7 D000293
650    _2
$a dospělí $7 D000328
650    _2
$a butyráty $x metabolismus $7 D002087
650    _2
$a DNA bakterií $x genetika $7 D004269
650    _2
$a feces $x mikrobiologie $7 D005243
650    _2
$a střevní mikroflóra $x genetika $7 D000069196
650    _2
$a genová dávka $7 D018628
650    12
$a bakteriální geny $7 D005798
650    _2
$a lidé $7 D006801
650    _2
$a lidé středního věku $7 D008875
650    _2
$a obezita $x mikrobiologie $7 D009765
650    _2
$a fenotyp $7 D010641
650    _2
$a fylogeneze $7 D010802
650    12
$a polymerázová řetězová reakce $7 D016133
650    _2
$a reprodukovatelnost výsledků $7 D015203
650    _2
$a neparametrická statistika $7 D018709
650    _2
$a vegani $7 D000066989
650    _2
$a mladý dospělý $7 D055815
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Heczkova, Marie $u Institute for Clinical and Experimental Medicine, Videnska 1958, 140 21 Prague 4, Czech Republic
700    1_
$a Modos, Istvan $u Institute for Clinical and Experimental Medicine, Videnska 1958, 140 21 Prague 4, Czech Republic
700    1_
$a Videnska, Petra $u RECETOX, Faculty of Science Masaryk University, Kamenice 753, 625 00 Brno, Czech Republic
700    1_
$a Splichalova, Petra $u RECETOX, Faculty of Science Masaryk University, Kamenice 753, 625 00 Brno, Czech Republic
700    1_
$a Pelantova, Helena $u Institute of Microbiology, AS CR, Videnska 1083, 142 20 Prague 4, Czech Republic
700    1_
$a Kuzma, Marek $u Institute of Microbiology, AS CR, Videnska 1083, 142 20 Prague 4, Czech Republic $u Department of Analytical Chemistry, Faculty of Science, Palacky University Olomouc, 17. Listopadu 1192/12, 779 00 Olomouc, Czech Republic
700    1_
$a Gojda, Jan $u Department of Internal Medicine, Kralovske Vinohrady University Hospital and Third Faculty of Medicine, Charles University, Srobarova 1150, 100 34 Prague 10, Czech Republic $1 https://orcid.org/0000000279955947
700    1_
$a Cahova, Monika $u Institute for Clinical and Experimental Medicine, Videnska 1958, 140 21 Prague 4, Czech Republic
773    0_
$w MED00188737 $t Biomolecules $x 2218-273X $g Roč. 11, č. 9 (2021)
856    41
$u https://pubmed.ncbi.nlm.nih.gov/34572516 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20220425 $b ABA008
991    __
$a 20220506131313 $b ABA008
999    __
$a ok $b bmc $g 1789704 $s 1163428
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2021 $b 11 $c 9 $e 20210902 $i 2218-273X $m Biomolecules $n Biomolecules $x MED00188737
LZP    __
$a Pubmed-20220425

Najít záznam

Citační ukazatele

Možnosti archivace