Over the past decades, the in vitro use of pluripotent cell lines gained a crucial role in toxicology, preclinical drug testing and developmental biology. NTERA2 clone D1 cells were identified as pluripotent cells with high potential for neural differentiation. Although they are commonly used cellular sources in neuropharmacology and neurodevelopmental studies, their endodermal and mesodermal differentiation potential awaits further characterization. Here, we devised improved protocols for hepatogenic and osteogenic differentiation of NTERA2 clone D1 cells. Our in vitro differentiation assays showed significant up-regulation of multiple hepatogenic markers. We also observed robust mineralization and osteogenic marker expression of NTERA2 clone D1 cells upon in vitro osteogenic induction. These results suggest that NTERA2 clone D1 cells may be utilized as an in vitro model system to study various aspects of liver biology and osteogenesis. In addition, tri-lineage differentiation of NTERA2 clone D1 cells may serve as a simple experimental control system when validating pluripotency of other cell types.

Department of Physiology Faculty of Medicine Semmelweis University Budapest Hungary

Roska Tamás Doctoral School of Science and Technology Faculty of Information Technology and Bionics Pázmány Péter Catholic University Budapest Hungary

Stem Cell Research Laboratory Department of Dermatology Venereology and Dermatooncology Faculty of Medicine Semmelweis University Budapest Hungary

Erratum in 2023, roč. 69, č. 1, s. 40