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mRNA Subtype of Cancer-Associated Fibroblasts Significantly Affects Key Characteristics of Head and Neck Cancer Cells
B. Peltanová, H. Holcová Polanská, M. Raudenská, J. Balvan, J. Navrátil, T. Vičar, J. Gumulec, B. Čechová, M. Kräter, J. Guck, D. Kalfeřt, M. Grega, J. Plzák, J. Betka, M. Masařík
Jazyk angličtina Země Švýcarsko
Typ dokumentu časopisecké články
Grantová podpora
18-03978S
Czech Science Foundation
NV18-08-00229
Czech Health Research Council
FW03010186
Technology Agency of the Czech Republic
NLK
Directory of Open Access Journals
od 2010
Free Medical Journals
od 2009
PubMed Central
od 2009
Europe PubMed Central
od 2009
ProQuest Central
od 2009-01-01
Open Access Digital Library
od 2009-01-01
Open Access Digital Library
od 2009-01-01
ROAD: Directory of Open Access Scholarly Resources
od 2009
PubMed
35565415
DOI
10.3390/cancers14092286
Knihovny.cz E-zdroje
- Publikační typ
- časopisecké články MeSH
Head and neck squamous cell carcinomas (HNSCC) belong among severe and highly complex malignant diseases showing a high level of heterogeneity and consequently also a variance in therapeutic response, regardless of clinical stage. Our study implies that the progression of HNSCC may be supported by cancer-associated fibroblasts (CAFs) in the tumour microenvironment (TME) and the heterogeneity of this disease may lie in the level of cooperation between CAFs and epithelial cancer cells, as communication between CAFs and epithelial cancer cells seems to be a key factor for the sustained growth of the tumour mass. In this study, we investigated how CAFs derived from tumours of different mRNA subtypes influence the proliferation of cancer cells and their metabolic and biomechanical reprogramming. We also investigated the clinicopathological significance of the expression of these metabolism-related genes in tissue samples of HNSCC patients to identify a possible gene signature typical for HNSCC progression. We found that the right kind of cooperation between cancer cells and CAFs is needed for tumour growth and progression, and only specific mRNA subtypes can support the growth of primary cancer cells or metastases. Specifically, during coculture, cancer cell colony supporting effect and effect of CAFs on cell stiffness of cancer cells are driven by the mRNA subtype of the tumour from which the CAFs are derived. The degree of colony-forming support is reflected in cancer cell glycolysis levels and lactate shuttle-related transporters.
BIOCEV 1st Faculty of Medicine Charles University Prumyslova 595 25250 Vestec Czech Republic
Department of Physiology Faculty of Medicine Masaryk University Kamenice 5 62500 Brno Czech Republic
Max Planck Institute for the Science of Light Staudtstraße 2 91058 Erlangen Germany
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