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A primer to traction force microscopy

A. Zancla, P. Mozetic, M. Orsini, G. Forte, A. Rainer

. 2022 ; 298 (5) : 101867. [pub] 20220326

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články, přehledy, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc22018587

Traction force microscopy (TFM) has emerged as a versatile technique for the measurement of single-cell-generated forces. TFM has gained wide use among mechanobiology laboratories, and several variants of the original methodology have been proposed. However, issues related to the experimental setup and, most importantly, data analysis of cell traction datasets may restrain the adoption of TFM by a wider community. In this review, we summarize the state of the art in TFM-related research, with a focus on the analytical methods underlying data analysis. We aim to provide the reader with a friendly compendium underlying the potential of TFM and emphasizing the methodological framework required for a thorough understanding of experimental data. We also compile a list of data analytics tools freely available to the scientific community for the furtherance of knowledge on this powerful technique.

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$a Traction force microscopy (TFM) has emerged as a versatile technique for the measurement of single-cell-generated forces. TFM has gained wide use among mechanobiology laboratories, and several variants of the original methodology have been proposed. However, issues related to the experimental setup and, most importantly, data analysis of cell traction datasets may restrain the adoption of TFM by a wider community. In this review, we summarize the state of the art in TFM-related research, with a focus on the analytical methods underlying data analysis. We aim to provide the reader with a friendly compendium underlying the potential of TFM and emphasizing the methodological framework required for a thorough understanding of experimental data. We also compile a list of data analytics tools freely available to the scientific community for the furtherance of knowledge on this powerful technique.
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$a Mozetic, Pamela $u Institute of Nanotechnology (NANOTEC), National Research Council, Lecce, Italy; Division of Neuroscience, Institute of Experimental Neurology, San Raffaele Scientific Institute, Milan, Italy
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$a Orsini, Monica $u Department of Engineering, Università degli Studi Roma Tre, Rome, Italy
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$a Forte, Giancarlo $u Center for Translational Medicine (CTM), International Clinical Research Center (ICRC), St Anne's University Hospital, Brno, Czechia. Electronic address: giancarlo.forte@fnusa.cz
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$a Rainer, Alberto $u Department of Engineering, Università Campus Bio-Medico di Roma, Rome, Italy; Institute of Nanotechnology (NANOTEC), National Research Council, Lecce, Italy. Electronic address: a.rainer@unicampus.it
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