Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Decreased Epicardial CTRP3 mRNA Levels in Patients with Type 2 Diabetes Mellitus and Coronary Artery Disease Undergoing Elective Cardiac Surgery: A Possible Association with Coronary Atherosclerosis

Z. Matloch, M. Mraz, BJ. Kasperova, H. Kratochvilova, P. Svoboda, I. Pleyerova, K. Reznickova, S. Norman, D. Hlavacek, J. Mahrik, P. Ivak, Z. Lacinova, I. Netuka, M. Haluzik

. 2022 ; 23 (17) : . [pub] 20220901

Jazyk angličtina Země Švýcarsko

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc22024589

Grantová podpora
LX22NPO5104 National Institute for Research of Metabolic and Cardiovascular Diseases
NV19-02-00118 Institute of Clinical and Experimental Medicine

(1) Background: C1q TNF-related protein 3 (CTRP3) is an adipokine with anti-inflammatory and cardioprotective properties. In our study, we explored changes in serum CTRP3 and its gene expression in epicardial (EAT) and subcutaneous (SAT) adipose tissue in patients with and without coronary artery disease (CAD) and type 2 diabetes mellitus (T2DM) undergoing elective cardiac surgery. (2) Methods: SAT, EAT, and blood samples were collected at the start and end of surgery from 34 patients: (i) 11 without CAD or T2DM, (ii) 14 with CAD and without T2DM, and (iii) 9 with both CAD and T2DM. mRNA levels of CTRP3 were assessed by quantitative reverse transcription PCR. Circulating levels of CTRP3 and other factors were measured using ELISA and Luminex Multiplex commercial kits. (3) Results: Baseline plasma levels of TNF-α and IL6 did not differ among the groups and increased at the end of surgery. Baseline circulating levels of CTRP3 did not differ among the groups and decreased after surgery. In contrast, baseline CTRP3 mRNA levels in EAT were significantly decreased in CAD/T2DM group, while no differences were found for TNF-α and IL6 gene expression. (4) Conclusions: Our data suggest that decreased EAT mRNA levels of CTRP3 could contribute to higher risk of atherosclerosis in patients with CAD and T2DM.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc22024589
003      
CZ-PrNML
005      
20221031100626.0
007      
ta
008      
221017s2022 sz f 000 0|eng||
009      
AR
024    7_
$a 10.3390/ijms23179988 $2 doi
035    __
$a (PubMed)36077376
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a sz
100    1_
$a Matloch, Zdenek $u Department of Cardiothoracic Anaesthesia and Intensive Care, The James Cook University Hospital, Marton Road, Middlesbrough TS4 3BW, UK $u First Faculty of Medicine, Charles University, Katerinska 32, 121 08 Prague, Czech Republic
245    10
$a Decreased Epicardial CTRP3 mRNA Levels in Patients with Type 2 Diabetes Mellitus and Coronary Artery Disease Undergoing Elective Cardiac Surgery: A Possible Association with Coronary Atherosclerosis / $c Z. Matloch, M. Mraz, BJ. Kasperova, H. Kratochvilova, P. Svoboda, I. Pleyerova, K. Reznickova, S. Norman, D. Hlavacek, J. Mahrik, P. Ivak, Z. Lacinova, I. Netuka, M. Haluzik
520    9_
$a (1) Background: C1q TNF-related protein 3 (CTRP3) is an adipokine with anti-inflammatory and cardioprotective properties. In our study, we explored changes in serum CTRP3 and its gene expression in epicardial (EAT) and subcutaneous (SAT) adipose tissue in patients with and without coronary artery disease (CAD) and type 2 diabetes mellitus (T2DM) undergoing elective cardiac surgery. (2) Methods: SAT, EAT, and blood samples were collected at the start and end of surgery from 34 patients: (i) 11 without CAD or T2DM, (ii) 14 with CAD and without T2DM, and (iii) 9 with both CAD and T2DM. mRNA levels of CTRP3 were assessed by quantitative reverse transcription PCR. Circulating levels of CTRP3 and other factors were measured using ELISA and Luminex Multiplex commercial kits. (3) Results: Baseline plasma levels of TNF-α and IL6 did not differ among the groups and increased at the end of surgery. Baseline circulating levels of CTRP3 did not differ among the groups and decreased after surgery. In contrast, baseline CTRP3 mRNA levels in EAT were significantly decreased in CAD/T2DM group, while no differences were found for TNF-α and IL6 gene expression. (4) Conclusions: Our data suggest that decreased EAT mRNA levels of CTRP3 could contribute to higher risk of atherosclerosis in patients with CAD and T2DM.
650    _2
$a tuková tkáň $x metabolismus $7 D000273
650    12
$a kardiochirurgické výkony $7 D006348
650    12
$a nemoci koronárních tepen $x komplikace $x genetika $x chirurgie $7 D003324
650    12
$a diabetes mellitus 2. typu $x komplikace $x genetika $x chirurgie $7 D003924
650    _2
$a lidé $7 D006801
650    _2
$a interleukin-6 $x metabolismus $7 D015850
650    _2
$a perikard $x metabolismus $7 D010496
650    _2
$a messenger RNA $x metabolismus $7 D012333
650    _2
$a TNF-alfa $x genetika $x metabolismus $7 D014409
655    _2
$a časopisecké články $7 D016428
700    1_
$a Mraz, Milos $u Diabetes Centre, Institute for Clinical and Experimental Medicine, Videnska 1958/9, 140 21 Prague, Czech Republic
700    1_
$a Kasperova, Barbora Judita $u Diabetes Centre, Institute for Clinical and Experimental Medicine, Videnska 1958/9, 140 21 Prague, Czech Republic $1 https://orcid.org/0000000345781093
700    1_
$a Kratochvilova, Helena $u Institute of Medical Biochemistry and Laboratory Diagnostics, First Faculty of Medicine, Charles University and General University Hospital, U Nemocnice 499/2, 128 08 Prague, Czech Republic $u Experimental Medicine Centre, Institute for Clinical and Experimental Medicine, Videnska 1958/9, 140 21 Prague, Czech Republic
700    1_
$a Svoboda, Petr $u Experimental Medicine Centre, Institute for Clinical and Experimental Medicine, Videnska 1958/9, 140 21 Prague, Czech Republic $u Department of Biochemistry and Microbiology, University of Chemistry and Technology Prague, Technicka 5, 166 28 Prague, Czech Republic $1 https://orcid.org/0000000172974208
700    1_
$a Pleyerova, Iveta $u Experimental Medicine Centre, Institute for Clinical and Experimental Medicine, Videnska 1958/9, 140 21 Prague, Czech Republic $1 https://orcid.org/0000000268941558
700    1_
$a Reznickova, Katerina $u Diabetes Centre, Institute for Clinical and Experimental Medicine, Videnska 1958/9, 140 21 Prague, Czech Republic $1 https://orcid.org/0000000337103866
700    1_
$a Norman, Sarah $u Department of Anaesthesia, The James Cook University Hospital, Marton Road, Middlesbrough TS4 3BW, UK
700    1_
$a Hlavacek, Daniel $u Department of Cardiac Surgery, Institute for Clinical and Experimental Medicine, Videnska 1958/9, 140 21 Prague, Czech Republic $1 https://orcid.org/0000000184531666
700    1_
$a Mahrik, Jakub $u Department of Cardiac Surgery, Institute for Clinical and Experimental Medicine, Videnska 1958/9, 140 21 Prague, Czech Republic $1 https://orcid.org/0000000291631881
700    1_
$a Ivak, Peter $u Department of Cardiac Surgery, Institute for Clinical and Experimental Medicine, Videnska 1958/9, 140 21 Prague, Czech Republic $u Department of Physiology, Third Faculty of Medicine, Charles University, Ruska 87, 100 00 Prague, Czech Republic
700    1_
$a Lacinova, Zdenka $u Institute of Medical Biochemistry and Laboratory Diagnostics, First Faculty of Medicine, Charles University and General University Hospital, U Nemocnice 499/2, 128 08 Prague, Czech Republic $u Experimental Medicine Centre, Institute for Clinical and Experimental Medicine, Videnska 1958/9, 140 21 Prague, Czech Republic
700    1_
$a Netuka, Ivan $u Department of Cardiac Surgery, Institute for Clinical and Experimental Medicine, Videnska 1958/9, 140 21 Prague, Czech Republic
700    1_
$a Haluzik, Martin $u Diabetes Centre, Institute for Clinical and Experimental Medicine, Videnska 1958/9, 140 21 Prague, Czech Republic $u Institute of Medical Biochemistry and Laboratory Diagnostics, First Faculty of Medicine, Charles University and General University Hospital, U Nemocnice 499/2, 128 08 Prague, Czech Republic
773    0_
$w MED00176142 $t International journal of molecular sciences $x 1422-0067 $g Roč. 23, č. 17 (2022)
856    41
$u https://pubmed.ncbi.nlm.nih.gov/36077376 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20221017 $b ABA008
991    __
$a 20221031100623 $b ABA008
999    __
$a ok $b bmc $g 1854360 $s 1175879
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2022 $b 23 $c 17 $e 20220901 $i 1422-0067 $m International journal of molecular sciences $n Int J Mol Sci $x MED00176142
GRA    __
$a LX22NPO5104 $p National Institute for Research of Metabolic and Cardiovascular Diseases
GRA    __
$a NV19-02-00118 $p Institute of Clinical and Experimental Medicine
LZP    __
$a Pubmed-20221017

Najít záznam

Citační ukazatele

Pouze přihlášení uživatelé

Možnosti archivace