More than half of the hospital-associated infections worldwide are related to the adhesion of bacteria cells to biomedical devices and implants. To prevent these infections, it is crucial to modify biomaterial surfaces to develop the antibacterial property. In this study, chitosan (CS) and chondroitin sulfate (ChS) were chosen as antibacterial coating materials on polylactic acid (PLA) surfaces. Plasma-treated PLA surfaces were coated with CS either direct coating method or the carbodiimide coupling method. As a next step for the combined saccharide coating, CS grafted samples were immersed in ChS solution, which resulted in the polyelectrolyte complex (PEC) formation. Also in this experiment, to test the drug loading and releasing efficiency of the thin film coatings, CS grafted samples were immersed into lomefloxacin-containing ChS solution. The successful modifications were confirmed by elemental composition analysis (XPS), surface topography images (SEM), and hydrophilicity change (contact angle measurements). The carbodiimide coupling resulted in higher CS grafting on the PLA surface. The coatings with the PEC formation between CS-ChS showed improved activity against the bacteria strains than the separate coatings. Moreover, these interactions increased the lomefloxacin amount adhered to the film coatings and extended the drug release profile. Finally, the zone of inhibition test confirmed that the CS-ChS coating showed a contact killing mechanism while drug-loaded films have a dual killing mechanism, which includes contact, and release killing.

Centre of Polymer Systems University Institute Tomas Bata University in Zlin Nam T G M 5555 76001 Zlin Czech Republic

Department of Surface Engineering Jozef Stefan Institute Jamova Cesta 39 1000 Ljubljana Slovenia

Faculty of Technology Tomas Bata University in Zlín Vavreckova 275 76001 Zlin Czech Republic

National Center for Micro and Nanofabrication Technical University of Denmark Building 347 East Ørsteds Plads 2800 Kongens Lyngby Denmark

000      

00000naa a2200000 a 4500

001      

bmc22024890

003      

CZ-PrNML

005      

20221031100601.0

007      

ta

008      

221017s2022 sz f 000 0|eng||

009      

AR

024    7_

$a 10.3390/ijms23158821 $2 doi

035    __

$a (PubMed)35955952

040    __

$a ABA008 $b cze $d ABA008 $e AACR2

041    0_

$a eng

044    __

$a sz

100    1_

$a Karakurt, Ilkay $u Centre of Polymer Systems, University Institute, Tomas Bata University in Zlin, Nam. T.G.M. 5555, 76001 Zlin, Czech Republic $1 https://orcid.org/0000000199832357

245    10

$a Effect of Saccharides Coating on Antibacterial Potential and Drug Loading and Releasing Capability of Plasma Treated Polylactic Acid Films / $c I. Karakurt, K. Ozaltin, H. Pištěková, D. Vesela, J. Michael-Lindhard, P. Humpolícek, M. Mozetič, M. Lehocky

520    9_

$a More than half of the hospital-associated infections worldwide are related to the adhesion of bacteria cells to biomedical devices and implants. To prevent these infections, it is crucial to modify biomaterial surfaces to develop the antibacterial property. In this study, chitosan (CS) and chondroitin sulfate (ChS) were chosen as antibacterial coating materials on polylactic acid (PLA) surfaces. Plasma-treated PLA surfaces were coated with CS either direct coating method or the carbodiimide coupling method. As a next step for the combined saccharide coating, CS grafted samples were immersed in ChS solution, which resulted in the polyelectrolyte complex (PEC) formation. Also in this experiment, to test the drug loading and releasing efficiency of the thin film coatings, CS grafted samples were immersed into lomefloxacin-containing ChS solution. The successful modifications were confirmed by elemental composition analysis (XPS), surface topography images (SEM), and hydrophilicity change (contact angle measurements). The carbodiimide coupling resulted in higher CS grafting on the PLA surface. The coatings with the PEC formation between CS-ChS showed improved activity against the bacteria strains than the separate coatings. Moreover, these interactions increased the lomefloxacin amount adhered to the film coatings and extended the drug release profile. Finally, the zone of inhibition test confirmed that the CS-ChS coating showed a contact killing mechanism while drug-loaded films have a dual killing mechanism, which includes contact, and release killing.

650    _2

$a antibakteriální látky $x farmakologie $7 D000900

650    _2

$a karbodiimidy $x farmakologie $7 D002234

650    12

$a chitosan $x farmakologie $7 D048271

650    _2

$a biokompatibilní potahované materiály $x farmakologie $7 D020099

650    _2

$a polyestery $x farmakologie $7 D011091

650    12

$a Staphylococcus aureus $7 D013211

655    _2

$a časopisecké články $7 D016428

700    1_

$a Ozaltin, Kadir $u Centre of Polymer Systems, University Institute, Tomas Bata University in Zlin, Nam. T.G.M. 5555, 76001 Zlin, Czech Republic $1 https://orcid.org/0000000276195321

700    1_

$a Pištěková, Hana $u Centre of Polymer Systems, University Institute, Tomas Bata University in Zlin, Nam. T.G.M. 5555, 76001 Zlin, Czech Republic

700    1_

$a Vesela, Daniela $u Centre of Polymer Systems, University Institute, Tomas Bata University in Zlin, Nam. T.G.M. 5555, 76001 Zlin, Czech Republic

700    1_

$a Michael-Lindhard, Jonas $u National Center for Micro- and Nanofabrication, Technical University of Denmark, Building 347 East, Ørsteds Plads, 2800 Kongens Lyngby, Denmark

700    1_

$a Humpolícek, Petr $u Centre of Polymer Systems, University Institute, Tomas Bata University in Zlin, Nam. T.G.M. 5555, 76001 Zlin, Czech Republic $u Faculty of Technology, Tomas Bata University in Zlín, Vavreckova 275, 76001 Zlin, Czech Republic $1 https://orcid.org/0000000268376878

700    1_

$a Mozetič, Miran $u Department of Surface Engineering, Jozef Stefan Institute, Jamova Cesta 39, 1000 Ljubljana, Slovenia $1 https://orcid.org/0000000235293371

700    1_

$a Lehocky, Marian $u Centre of Polymer Systems, University Institute, Tomas Bata University in Zlin, Nam. T.G.M. 5555, 76001 Zlin, Czech Republic $u Faculty of Technology, Tomas Bata University in Zlín, Vavreckova 275, 76001 Zlin, Czech Republic $1 https://orcid.org/0000000253685029

773    0_

$w MED00176142 $t International journal of molecular sciences $x 1422-0067 $g Roč. 23, č. 15 (2022)

856    41

$u https://pubmed.ncbi.nlm.nih.gov/35955952 $y Pubmed

910    __

$a ABA008 $b sig $c sign $y p $z 0

990    __

$a 20221017 $b ABA008

991    __

$a 20221031100559 $b ABA008

999    __

$a ok $b bmc $g 1854544 $s 1176180

BAS    __

$a 3

BAS    __

$a PreBMC

BMC    __

$a 2022 $b 23 $c 15 $e 20220808 $i 1422-0067 $m International journal of molecular sciences $n Int J Mol Sci $x MED00176142

LZP    __

$a Pubmed-20221017