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Comprehensive ECG reference intervals in C57BL/6N substrains provide a generalizable guide for cardiac electrophysiology studies in mice
MA. Oestereicher, JM. Wotton, S. Ayabe, G. Bou About, TK. Cheng, JH. Choi, D. Clary, EM. Dew, L. Elfertak, A. Guimond, H. Haseli Mashhadi, JD. Heaney, L. Kelsey, P. Keskivali-Bond, F. Lopez Gomez, S. Marschall, M. McFarland, H. Meziane, V. Munoz...
Language English Country United States
Document type Journal Article, Review
Grant support
UM1 HG006348
NHGRI NIH HHS - United States
NLK
ProQuest Central
from 1997-01-01 to 1 year ago
Medline Complete (EBSCOhost)
from 2000-01-01 to 1 year ago
Health & Medicine (ProQuest)
from 1997-01-01 to 1 year ago
- MeSH
- Electrophysiologic Techniques, Cardiac * MeSH
- Electrocardiography * MeSH
- Mice, Inbred Strains MeSH
- Mice, Inbred C57BL MeSH
- Mice MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Review MeSH
Reference ranges provide a powerful tool for diagnostic decision-making in clinical medicine and are enormously valuable for understanding normality in pre-clinical scientific research that uses in vivo models. As yet, there are no published reference ranges for electrocardiography (ECG) in the laboratory mouse. The first mouse-specific reference ranges for the assessment of electrical conduction are reported herein generated from an ECG dataset of unprecedented scale. International Mouse Phenotyping Consortium data from over 26,000 conscious or anesthetized C57BL/6N wildtype control mice were stratified by sex and age to develop robust ECG reference ranges. Interesting findings include that heart rate and key elements from the ECG waveform (RR-, PR-, ST-, QT-interval, QT corrected, and QRS complex) demonstrate minimal sexual dimorphism. As expected, anesthesia induces a decrease in heart rate and was shown for both inhalation (isoflurane) and injectable (tribromoethanol) anesthesia. In the absence of pharmacological, environmental, or genetic challenges, we did not observe major age-related ECG changes in C57BL/6N-inbred mice as the differences in the reference ranges of 12-week-old compared to 62-week-old mice were negligible. The generalizability of the C57BL/6N substrain reference ranges was demonstrated by comparison with ECG data from a wide range of non-IMPC studies. The close overlap in data from a wide range of mouse strains suggests that the C57BL/6N-based reference ranges can be used as a robust and comprehensive indicator of normality. We report a unique ECG reference resource of fundamental importance for any experimental study of cardiac function in mice.
German Center for Diabetes Research Ingolstädter Landstrasse 1 85764 Neuherberg Germany
Integrative Physiology Baylor College of Medicine One Baylor Plaza Houston TX 77030 USA
Molecular and Human Genetics Baylor College of Medicine One Baylor Plaza Houston TX 77030 USA
Mouse Biology Program University of California 2795 2nd Street Suite 400 Davis CA 95618 USA
The Centre for Phenogenomics The Hospital for Sick Children Toronto ON M5T 3H7 Canada
The Jackson Laboratory 600 Main Street Bar Harbor ME 04609 USA
The Mary Lyon Centre MRC Harwell Harwell Campus Oxfordshire OX11 0RD UK
References provided by Crossref.org
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