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Je něco špatně v tomto záznamu ?
Disclosing quantitative RT-PCR raw data during manuscript submission: a call for action
A. Untergasser, J. Hellemans, MW. Pfaffl, JM. Ruijter, MJB. van den Hoff, MP. Dragomir, D. Adamoski, SMG. Dias, RM. Reis, M. Ferracin, E. Dias-Neto, I. Marsh, M. Kubista, M. Fabbri, A. Goel, O. Slabý, E. Knutsen, B. Chen, M. Negrini, K. Mimori,...
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, práce podpořená grantem
NLK
Directory of Open Access Journals
od 2017
Free Medical Journals
od 2007 do Před 1 rokem
PubMed Central
od 2007
Europe PubMed Central
od 2007
ProQuest Central
od 2007-06-01
Wiley-Blackwell Open Access Titles
od 2007
PubMed
36916500
DOI
10.1002/1878-0261.13418
Knihovny.cz E-zdroje
- MeSH
- kvantitativní polymerázová řetězová reakce metody MeSH
- lidé MeSH
- polymerázová řetězová reakce s reverzní transkripcí MeSH
- reprodukovatelnost výsledků MeSH
- RNA * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Accuracy and transparency of scientific data are becoming more and more relevant with the increasing concern regarding the evaluation of data reproducibility in many research areas. This concern is also true for quantifying coding and noncoding RNAs, with the remarkable increase in publications reporting RNA profiling and sequencing studies. To address the problem, we propose the following recommendations: (a) accurate documentation of experimental procedures in Materials and methods (and not only in the supplementary information, as many journals have a strict mandate for making Materials and methods as visible as possible in the main text); (b) submission of RT-qPCR raw data for all experiments reported; and (c) adoption of a unified, simple format for submitted RT-qPCR raw data. The Real-time PCR Data Essential Spreadsheet Format (RDES) was created for this purpose.
B's PT Government Associate Laboratory Braga Guimarães Portugal
Berlin Institute of Health Germany
Brazilian Biosciences National Laboratory Campinas Brazil
Center for Cancer and Immunology Research Children's National Hospital Washington DC USA
City of Hope Comprehensive Cancer Center Duarte CA USA
Department of Biomolecular Medicine Ghent University Belgium
Department of Comprehensive Cancer Care Masaryk Memorial Cancer Institute Brno Czech Republic
Department of Medical and Surgical Sciences University of Bologna Italy
Department of Medical Biology Amsterdam UMC location AMC University of Amsterdam The Netherlands
Department of Pharmaceutics University of Florida College of Pharmacy Gainesville FL USA
Department of Surgery Kyushu University Beppu Hospital Japan
Division of Oncology Department of Internal Medicine Medical University of Graz Austria
Editorial Manager Molecular Oncology Cambridge UK
Genomics Core Facility European Molecular Biology Laboratory Heidelberg Germany
German Cancer Research Center Heidelberg Germany
Institute of Biotechnology CAS v v i Vestec Czech Republic
IRCCS Azienda Ospedaliero Universitaria di Bologna Italy
Medical Technology Research Centre Anglia Ruskin University Chelmsford Essex UK
Molecular Oncology Research Center Barretos Cancer Hospital Brazil
National Measurement Laboratory LGC Teddington UK
OncoRNALab Cancer Research Institute Ghent Belgium
TATAA Biocenter AB Göteborg Sweden
The Center for Non codingRNAs University of Texas MD Anderson Cancer Center Houston TX USA
Translational Oncology University Hospital of Augsburg Stenglinstrasse Germany
Zentrum für Molekulare Biologie der Universität Heidelberg Germany
Citace poskytuje Crossref.org
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