-
Je něco špatně v tomto záznamu ?
Flow cytometry-based method using diversity of cytokine production differentiates between Mycobacterium tuberculosis infection and disease
K. Dolezalova, P. Hadlova, M. Ibrahimova, J. Golias, L. Baca, E. Kopecka, M. Sukholytka, M. Koziar Vasakova
Jazyk angličtina
Typ dokumentu časopisecké články, srovnávací studie
- MeSH
- antigeny bakteriální imunologie MeSH
- biologické markery krev MeSH
- CD4-pozitivní T-lymfocyty * imunologie MeSH
- CD8-pozitivní T-lymfocyty * imunologie MeSH
- cytokiny krev metabolismus MeSH
- diferenciální diagnóza MeSH
- dítě MeSH
- dospělí MeSH
- interferon gama * krev imunologie MeSH
- interleukin-2 * krev MeSH
- latentní tuberkulóza * diagnóza imunologie mikrobiologie MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- Mycobacterium tuberculosis * imunologie MeSH
- pilotní projekty MeSH
- plicní tuberkulóza diagnóza imunologie mikrobiologie krev MeSH
- prediktivní hodnota testů MeSH
- předškolní dítě MeSH
- průtoková cytometrie * metody MeSH
- senioři MeSH
- test pomocí interferonu gama metody MeSH
- TNF-alfa krev MeSH
- Check Tag
- dítě MeSH
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- předškolní dítě MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
Authors present a pilot study of the development of innovative flow cytometry-based assay with a potential for use in tuberculosis diagnostics. Currently available tests do not provide robust discrimination between latent tuberculosis infection (TBI) and tuberculosis disease (TB). The desired application is to distinguish between the two conditions by evaluating the production of a combination of three cytokines: IL-2 (interleukin-2), IFNɣ (interferon gamma) and TNFɑ (tumor necrosis factor alpha) in CD4+ and CD8+ T cells. The study was conducted on 68 participants, divided into two arms according to age (paediatric and adults). Each arm was further split into three categories (non-infection (NI), TBI, TB) based on the immune reaction to Mycobacterium tuberculosis (M.tb) after a close contact with pulmonary TB. Each blood sample was stimulated with specific M.tb antigens present in QuantiFERON tubes (TB1 and TB2). We inferred TBI or TB based on the predominant cytokine response of the CD4+ and/or CD8+ T cells. Significant differences were detected between the NI, TBI and the TB groups in TB1 in the CD4+TNFɑ+parameter in children. Along with IL-2, TNFɑ seems to be the most promising diagnostic marker in both CD4+and CD8+ T cells. However, more detailed analyses on larger cohorts are needed to confirm the observed tendencies.
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc24013368
- 003
- CZ-PrNML
- 005
- 20240905133859.0
- 007
- ta
- 008
- 240725e20240508Sco f 000 0|00 0|
- 009
- AR
- 024 7_
- $a 10.1016/j.tube.2024.102518 $2 doi
- 035 __
- $a (PubMed)38739968
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a Scotland
- 100 1_
- $a Dolezalova, Karolina $u Department of Paediatrics of the First Faculty of Medicine, Charles University, Thomayer University Hospital, Prague, Czech Republic. Electronic address: karolina.dolezalova@ftn.cz
- 245 10
- $a Flow cytometry-based method using diversity of cytokine production differentiates between Mycobacterium tuberculosis infection and disease / $c K. Dolezalova, P. Hadlova, M. Ibrahimova, J. Golias, L. Baca, E. Kopecka, M. Sukholytka, M. Koziar Vasakova
- 520 9_
- $a Authors present a pilot study of the development of innovative flow cytometry-based assay with a potential for use in tuberculosis diagnostics. Currently available tests do not provide robust discrimination between latent tuberculosis infection (TBI) and tuberculosis disease (TB). The desired application is to distinguish between the two conditions by evaluating the production of a combination of three cytokines: IL-2 (interleukin-2), IFNɣ (interferon gamma) and TNFɑ (tumor necrosis factor alpha) in CD4+ and CD8+ T cells. The study was conducted on 68 participants, divided into two arms according to age (paediatric and adults). Each arm was further split into three categories (non-infection (NI), TBI, TB) based on the immune reaction to Mycobacterium tuberculosis (M.tb) after a close contact with pulmonary TB. Each blood sample was stimulated with specific M.tb antigens present in QuantiFERON tubes (TB1 and TB2). We inferred TBI or TB based on the predominant cytokine response of the CD4+ and/or CD8+ T cells. Significant differences were detected between the NI, TBI and the TB groups in TB1 in the CD4+TNFɑ+parameter in children. Along with IL-2, TNFɑ seems to be the most promising diagnostic marker in both CD4+and CD8+ T cells. However, more detailed analyses on larger cohorts are needed to confirm the observed tendencies.
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a dítě $7 D002648
- 650 12
- $a latentní tuberkulóza $x diagnóza $x imunologie $x mikrobiologie $7 D055985
- 650 12
- $a průtoková cytometrie $x metody $7 D005434
- 650 _2
- $a dospělí $7 D000328
- 650 12
- $a Mycobacterium tuberculosis $x imunologie $7 D009169
- 650 12
- $a CD8-pozitivní T-lymfocyty $x imunologie $7 D018414
- 650 _2
- $a mužské pohlaví $7 D008297
- 650 _2
- $a ženské pohlaví $7 D005260
- 650 12
- $a CD4-pozitivní T-lymfocyty $x imunologie $7 D015496
- 650 12
- $a interleukin-2 $x krev $7 D007376
- 650 _2
- $a pilotní projekty $7 D010865
- 650 _2
- $a mladiství $7 D000293
- 650 _2
- $a mladý dospělý $7 D055815
- 650 _2
- $a lidé středního věku $7 D008875
- 650 12
- $a interferon gama $x krev $x imunologie $7 D007371
- 650 _2
- $a předškolní dítě $7 D002675
- 650 _2
- $a cytokiny $x krev $x metabolismus $7 D016207
- 650 _2
- $a biologické markery $x krev $7 D015415
- 650 _2
- $a TNF-alfa $x krev $7 D014409
- 650 _2
- $a diferenciální diagnóza $7 D003937
- 650 _2
- $a plicní tuberkulóza $x diagnóza $x imunologie $x mikrobiologie $x krev $7 D014397
- 650 _2
- $a prediktivní hodnota testů $7 D011237
- 650 _2
- $a antigeny bakteriální $x imunologie $7 D000942
- 650 _2
- $a test pomocí interferonu gama $x metody $7 D059425
- 650 _2
- $a senioři $7 D000368
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a srovnávací studie $7 D003160
- 700 1_
- $a Hadlova, Petra $u Childhood Leukaemia Investigation Prague (CLIP), 2nd Faculty of Medicine, Charles University, Prague, Czech Republic
- 700 1_
- $a Ibrahimova, Marketa $u Laboratory of Immunology, Thomayer University Hospital, Prague, Czech Republic
- 700 1_
- $a Golias, Jaroslav $u Laboratory of Immunology, Thomayer University Hospital, Prague, Czech Republic
- 700 1_
- $a Baca, Lubos $u Department of Paediatrics of the First Faculty of Medicine, Charles University, Thomayer University Hospital, Prague, Czech Republic
- 700 1_
- $a Kopecka, Emilia $u Department of Respiratory Medicine of the First Faculty of Medicine Charles University, Thomayer University Hospital, Prague, Czech Republic
- 700 1_
- $a Sukholytka, Mariia $u Department of Respiratory Medicine of the First Faculty of Medicine Charles University, Thomayer University Hospital, Prague, Czech Republic
- 700 1_
- $a Koziar Vasakova, Martina $u Department of Respiratory Medicine of the First Faculty of Medicine Charles University, Thomayer University Hospital, Prague, Czech Republic
- 773 0_
- $w MED00007425 $t Tuberculosis $x 1873-281X $g Roč. 147 (20240508), s. 102518
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/38739968 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y - $z 0
- 990 __
- $a 20240725 $b ABA008
- 991 __
- $a 20240905133853 $b ABA008
- 999 __
- $a ok $b bmc $g 2143277 $s 1225234
- BAS __
- $a 3
- BAS __
- $a PreBMC-MEDLINE
- BMC __
- $a 2024 $b 147 $c - $d 102518 $e 20240508 $i 1873-281X $m Tuberculosis $n Tuberculosis (Edinb) $x MED00007425
- LZP __
- $a Pubmed-20240725
