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Antifungal and antibiofilm effect of duloxetine hydrochloride against Cryptococcus neoformans and Cryptococcus gattii
AR. Rehem, LR. da Gama Viveiro, EL. De Souza Santos, PHF. do Carmo, NS. da Silva, JC. Junqueira, L. Scorzoni
Jazyk angličtina Země Česko
Typ dokumentu časopisecké články
- MeSH
- antifungální látky * farmakologie MeSH
- biofilmy * účinky léků MeSH
- Cryptococcus gattii * účinky léků MeSH
- Cryptococcus neoformans * účinky léků růst a vývoj MeSH
- duloxetinum hydrochlorid * farmakologie MeSH
- kryptokokóza farmakoterapie mikrobiologie MeSH
- mikrobiální testy citlivosti * MeSH
- Publikační typ
- časopisecké články MeSH
Cryptococcosis is an invasive mycosis caused mainly by Cryptococcus gattii and C. neoformans and is treated with amphotericin B (AMB), fluconazole and 5-fluorocytosine. However, antifungal resistance, limited and toxic antifungal arsenal stimulate the search for therapeutic strategies such as drug repurposing. Among the repurposed drugs studied, the selective serotonin reuptake inhibitors (SSRIs) have shown activity against Cryptococcus spp. However, little is known about the antifungal effect of duloxetine hydrochloride (DH), a selective serotonin and norepinephrine reuptake inhibitor (SSNRI), against C. neoformans and C. gattii. In this study, DH inhibited the growth of several C. neoformans and C. gattii strains at concentrations ranging from 15.62 to 62.50 μg/mL. In addition, DH exhibited fungicidal activity ranging from 15.62 to 250 μg/mL. In biofilm, DH treatment reduced Cryptococcus spp. biomass at a level comparable to AMB, with a significant reduction (85%) for C. neoformans biofilms. The metabolic activity of C. neoformans and C. gattii biofilms decreased significantly (99%) after treatment with DH. Scanning electron micrographs confirmed the anti-biofilm activity of DH, as isolated cells could be observed after treatment. In conclusion, DH showed promising antifungal activity against planktonic cells and biofilms of C. neoformans and C. gattii, opening perspectives for further studies with DH in vivo.
Citace poskytuje Crossref.org
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