-
Something wrong with this record ?
Seeing the Spikes: The Future of Targetable Synthetic Voltage Sensors
T. Fiala, D. Sulzer, D. Sames
Language English Country United States
Document type Journal Article, Review, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't
Grant support
R01 DA007418
NIDA NIH HHS - United States
R01 MH122470
NIMH NIH HHS - United States
- MeSH
- Action Potentials physiology drug effects MeSH
- Fluorescent Dyes MeSH
- Humans MeSH
- Membrane Potentials physiology MeSH
- Neurons * physiology MeSH
- Voltage-Sensitive Dye Imaging methods trends MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Review MeSH
- Research Support, N.I.H., Extramural MeSH
Measuring the transduction of electrical signals within neurons is a key capability in neuroscience. Fluorescent voltage sensitive dyes (VSDs) were early tools that complemented classical electrophysiology by enabling the optical recording of membrane potential changes from many cells simultaneously. Recent advances in the VSD field have led to bright and highly sensitive sensors that can be targeted to the desired cell populations in live brain tissue. Despite this progress, recently, protein-based genetically encoded voltage indicators (GEVIs) have become the go-to tools for targeted voltage imaging in complex environments. In this Perspective, we summarize progress in developing targetable VSDs, discuss areas where these synthetic sensors are or could become relevant, and outline hurdles that need to be overcome to promote the routine use of targetable VSDs in neuroscience research.
Department of Chemistry Columbia University New York New York 10027 United States
Department of Chemistry Faculty of Science Masaryk University Kamenice 5 625 00 Brno Czech Republic
Laboratory of Organic Chemistry ETH Zürich D CHAB Vladimir Prelog Weg 3 8093 Zürich Switzerland
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc25009561
- 003
- CZ-PrNML
- 005
- 20250429135442.0
- 007
- ta
- 008
- 250415s2025 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1021/acschemneuro.4c00849 $2 doi
- 035 __
- $a (PubMed)39943826
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Fiala, Tomas $u Laboratory of Organic Chemistry, ETH Zürich, D-CHAB, Vladimir-Prelog-Weg 3, 8093 Zürich, Switzerland $u Department of Chemistry, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic $1 https://orcid.org/0000000169494561
- 245 10
- $a Seeing the Spikes: The Future of Targetable Synthetic Voltage Sensors / $c T. Fiala, D. Sulzer, D. Sames
- 520 9_
- $a Measuring the transduction of electrical signals within neurons is a key capability in neuroscience. Fluorescent voltage sensitive dyes (VSDs) were early tools that complemented classical electrophysiology by enabling the optical recording of membrane potential changes from many cells simultaneously. Recent advances in the VSD field have led to bright and highly sensitive sensors that can be targeted to the desired cell populations in live brain tissue. Despite this progress, recently, protein-based genetically encoded voltage indicators (GEVIs) have become the go-to tools for targeted voltage imaging in complex environments. In this Perspective, we summarize progress in developing targetable VSDs, discuss areas where these synthetic sensors are or could become relevant, and outline hurdles that need to be overcome to promote the routine use of targetable VSDs in neuroscience research.
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a lidé $7 D006801
- 650 12
- $a neurony $x fyziologie $7 D009474
- 650 _2
- $a zobrazování pomocí barviva citlivého na potenciál $x metody $x trendy $7 D056969
- 650 _2
- $a fluorescenční barviva $7 D005456
- 650 _2
- $a akční potenciály $x fyziologie $x účinky léků $7 D000200
- 650 _2
- $a membránové potenciály $x fyziologie $7 D008564
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a přehledy $7 D016454
- 655 _2
- $a Research Support, N.I.H., Extramural $7 D052061
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Sulzer, David $u Departments of Neurology, Psychiatry, and Pharmacology, Columbia University Irving Medical Center, New York, New York 10032, United States $u Department of Molecular Therapeutics, New York State Psychiatric Institute, New York, New York 10032, United States $1 https://orcid.org/0000000176320439
- 700 1_
- $a Sames, Dalibor $u Department of Chemistry, Columbia University, New York, New York 10027, United States $u The Zuckerman Mind Brain Behavior Institute, Columbia University, New York, New York 10027, United States $1 https://orcid.org/0000000169112260
- 773 0_
- $w MED00193636 $t ACS chemical neuroscience $x 1948-7193 $g Roč. 16, č. 5 (2025), s. 761-771
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/39943826 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y - $z 0
- 990 __
- $a 20250415 $b ABA008
- 991 __
- $a 20250429135437 $b ABA008
- 999 __
- $a ok $b bmc $g 2311130 $s 1246642
- BAS __
- $a 3
- BAS __
- $a PreBMC-MEDLINE
- BMC __
- $a 2025 $b 16 $c 5 $d 761-771 $e 20250213 $i 1948-7193 $m ACS chemical neuroscience $n ACS Chem Neurosci $x MED00193636
- GRA __
- $a R01 DA007418 $p NIDA NIH HHS $2 United States
- GRA __
- $a R01 MH122470 $p NIMH NIH HHS $2 United States
- LZP __
- $a Pubmed-20250415
