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The ReAct project: Analysis of data from 23 different laboratories to characterise DNA recovery given two sets of activity level propositions
P. Gill, AE. Fonneløp, T. Hicks, S. Xenophontos, M. Cariolou, R. van Oorschot, I. Buckel, V. Sukser, S. Papić, S. Merkaš, A. Kostic, AM. Pereira, C. Teutsch, C. Forsberg, C. Haas, E. Petkovski, F. Hass, J. Masek, J. Stosic, YS. Lee, CK. Syn, L....
Jazyk angličtina Země Nizozemsko
Typ dokumentu časopisecké články
- MeSH
- Bayesova věta * MeSH
- DNA fingerprinting * MeSH
- DNA * genetika MeSH
- laboratoře * MeSH
- lidé MeSH
- mikrosatelitní repetice MeSH
- pravděpodobnostní funkce MeSH
- soudní genetika metody MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
The ReAct (Recovery, Activity) project is an ENFSI (European Network of Forensic Science Institutes) supported initiative comprising a large consortium of laboratories. Here, the results from more than 23 laboratories are presented. The primary purpose was to design experiments simulating typical casework circumstances; collect data and to implement Bayesian networks to assess the value (i.e., likelihood ratio) of DNA results given activity level propositions. Two different experimental designs were used to simulate a robbery, where a screwdriver was used to force a door or window. Propositions and case information were chosen following laboratory feedback listing typical casework circumstances (included in the paper). In a direct transfer experiment, the defendant owned and used the screwdriver, but he did not force the door/window in question. An unknown person used the defendant's stolen screwdriver. In an indirect transfer experiment, the defendant neither owned, saw, nor used the screwdriver, nor did they force the door or window. For the second experiment, given the defence view, the defendant never held the screwdriver. We envisaged the situation where an object manipulated by the defendant (or the defendant himself/herself) would be touched by the unknown offender who would then force the window. It was found for the direct transfer experiment that unless a single contributor profile aligning with the known person's of interest profile was retrieved, the results did not allow to discriminate between propositions. On the other hand, for the indirect transfer experiment, both single and major contributor profiles that aligned with the person of interest (POI) supported the proposition that the person used the tool rather than an unknown person who had touched an object, when indeed the former was true. There was considerable variation in median recoveries of DNA between laboratories (between 200pg-5ng) for a given experiment if quantities are taken into account. These differences affect the likelihood ratios given activity level propositions. More than 2700 samples were analysed in the course of this study. Two different Bayesian Networks are made available via an open source application written in Shiny R: Shiny_React(). For comparison, all datasets were analysed using a qualitative method categorised into absent, single, major or other given contributors. The importance of standardising methods is emphasised, alongside the necessity of developing new approaches to assign the probability of laboratory-dependent DNA recovery. Freely accessible open databases play a crucial role in supporting these efforts.
Bundeskriminalamt Wiesbaden Germany
Cellmark Forensic Services 16 Blacklands Way Abingdon OX14 1DY UK
Centre for Ecological and Evolutionary Synthesis University of Oslo Norway
Comisaría General de Policía Científica DNA Laboratory Julián González Segador Madrid Spain
Department of Clinical Medicine University of Oslo Oslo Norway
Department of Forensic Medicine Sect Forensic Genetics University of Copenhagen Denmark
Department of Forensic Sciences Oslo University Hospital Oslo Norway
Eurofins Forensic Services 1 Dukes Green Avenue Feltham TW14 0LR UK
Eurofins Forensic Services Building 414 Birchwood Park WA3 4FW UK
Eurofins Forensic Services Sir Alec Jeffrey's Building Calder Park WF2 7UA UK
Forensic Genetics Service Laboratoire National de Santé 1 Rue Louis Rech L 3555 Dudelange Luxembourg
Forensic Science Centre Ivan Vučetić MoI Ilica 335 Zagreb Croatia
Forensic Science Institute Berlin Police Tempelhofer Damm 12 12101 Berlin Germany
Forensic Science Program The Pennsylvania State University PA 16801 USA
Health Sciences Authority 11 Outram Road Singapore 169078 Singapore
Institute of Legal Medicine Medical University of Innsbruck Muellerstrasse 44 6020 Innsbruck Austria
International Commission on Missing Persons The Hague The Netherlands
Landeskriminalamt Baden Württemberg Taubenheimstr 85 70372 Stuttgart Germany
Landeskriminalamt Rheinland Pfalz Valenciaplatz 1 7 D 55118 Mainz Germany
Metropolitan Police Service Forensic Services 109 Lambeth Road London SE1 7LP UK
National Bureau of Investigation Forensic Laboratory Tikkurilantie 30 01370 Vantaa Finland
National Forensic Centre Ministry of Interior Kneza Miloša 103 Belgrade Serbia
National Forensic Centre Swedish Police Authority Linköping SE 581 94 Sweden
School of Agriculture Biomedicine and Environment La Trobe University Bundoora Australia
The laboratory of Forensic Genetics The Cyprus Institute of Neurology and Genetics Nicosia Cyprus
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- $a Gill, Peter $u Department of Forensic Sciences, Oslo University Hospital, Oslo, Norway; Department of Clinical Medicine, University of Oslo, Oslo, Norway. Electronic address: peterd.gill@gmail.com
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