Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Bioenergetics of human spermatozoa in patients with testicular germ cell tumours

O. Simonik, B. Bryndova, VP. Sur, L. Ded, Z. Cockova, A. Benda, M. Qasemi, P. Pecina, A. Pecinova, D. Spevakova, T. Hradec, P. Skrobanek, Z. Ezrova, Z. Kratka, R. Kren, M. Jeseta, L. Boublikova, L. Zamecnik, T. Büchler, J. Neuzil, P. Postlerova,...

. 2025 ; 31 (1) : . [pub] 20250117

Jazyk angličtina Země Anglie, Velká Británie

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc25010233

Grantová podpora
NU20-03-00309 Ministry of Health of the Czech Republic
GA22-21082S Czech Science Foundation

In patients with testicular germ cell tumours (TGCT), sperm cryopreservation prior to anti-cancer treatment represents the main fertility preservation approach. However, it is associated with a low sperm recovery rate after thawing. Since sperm is a high-energy demanding cell, which is supplied by glycolysis and oxidative phosphorylation (OXPHOS), mitochondrial dysfunctionality can directly result in sperm anomalies. In this study, we investigated the bioenergetic pattern of cryopreserved sperm of TGCT patients in comparison with normozoospermic samples using two state-of-the-art methods: the Extracellular Flux Analyzer (XF Analyzer) and two-photon fluorescence lifetime imaging microscopy (2P-FLIM), in order to assess the contributions of OXPHOS and glycolysis to energy provision. A novel protocol for the combined measurement of OXPHOS (oxygen consumption rate: OCR) and glycolysis (extracellular acidification rate: ECAR) using the XF Analyzer was developed together with a unique customized AI-based approach for semiautomated processing of 2P-FLIM images. Our study delivers optimized low-HEPES modified human tubal fluid media (mHTF) for sperm handling during pre-analytical and analytical phases, to maintain sperm physiological parameters and optimal OCR, equivalent to OXPHOS. The negative effect of cryopreservation was signified by the deterioration of both bioenergetic pathways represented by modified OCR and ECAR curves and the derived parameters. This was true for normozoospermic as well as samples from TGCT patients, which showed even stronger damage within the respiratory chain compared to the level of glycolytic activity impairment. The impact of cryopreservation and pathology are supported by 2P-FLIM analysis, showing a significant decrease in bound NADH in contrast to unbound NAD(P)H, which reflects decreased metabolic activity in samples from TGCT patients. Our study provides novel insights into the impact of TGCT on sperm bioenergetics and delivers a verified protocol to be used for the assessment of human sperm metabolic activity, which can be a valuable tool for further research and clinical andrology.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc25010233
003      
CZ-PrNML
005      
20250429134845.0
007      
ta
008      
250415s2025 enk f 000 0|eng||
009      
AR
024    7_
$a 10.1093/molehr/gaaf005 $2 doi
035    __
$a (PubMed)40053689
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a enk
100    1_
$a Simonik, Ondrej $u Laboratory of Reproductive Biology, Institute of Biotechnology of the Czech Academy of Sciences, Vestec, Czech Republic $1 https://orcid.org/000000026577765X
245    10
$a Bioenergetics of human spermatozoa in patients with testicular germ cell tumours / $c O. Simonik, B. Bryndova, VP. Sur, L. Ded, Z. Cockova, A. Benda, M. Qasemi, P. Pecina, A. Pecinova, D. Spevakova, T. Hradec, P. Skrobanek, Z. Ezrova, Z. Kratka, R. Kren, M. Jeseta, L. Boublikova, L. Zamecnik, T. Büchler, J. Neuzil, P. Postlerova, K. Komrskova
520    9_
$a In patients with testicular germ cell tumours (TGCT), sperm cryopreservation prior to anti-cancer treatment represents the main fertility preservation approach. However, it is associated with a low sperm recovery rate after thawing. Since sperm is a high-energy demanding cell, which is supplied by glycolysis and oxidative phosphorylation (OXPHOS), mitochondrial dysfunctionality can directly result in sperm anomalies. In this study, we investigated the bioenergetic pattern of cryopreserved sperm of TGCT patients in comparison with normozoospermic samples using two state-of-the-art methods: the Extracellular Flux Analyzer (XF Analyzer) and two-photon fluorescence lifetime imaging microscopy (2P-FLIM), in order to assess the contributions of OXPHOS and glycolysis to energy provision. A novel protocol for the combined measurement of OXPHOS (oxygen consumption rate: OCR) and glycolysis (extracellular acidification rate: ECAR) using the XF Analyzer was developed together with a unique customized AI-based approach for semiautomated processing of 2P-FLIM images. Our study delivers optimized low-HEPES modified human tubal fluid media (mHTF) for sperm handling during pre-analytical and analytical phases, to maintain sperm physiological parameters and optimal OCR, equivalent to OXPHOS. The negative effect of cryopreservation was signified by the deterioration of both bioenergetic pathways represented by modified OCR and ECAR curves and the derived parameters. This was true for normozoospermic as well as samples from TGCT patients, which showed even stronger damage within the respiratory chain compared to the level of glycolytic activity impairment. The impact of cryopreservation and pathology are supported by 2P-FLIM analysis, showing a significant decrease in bound NADH in contrast to unbound NAD(P)H, which reflects decreased metabolic activity in samples from TGCT patients. Our study provides novel insights into the impact of TGCT on sperm bioenergetics and delivers a verified protocol to be used for the assessment of human sperm metabolic activity, which can be a valuable tool for further research and clinical andrology.
650    _2
$a lidé $7 D006801
650    _2
$a mužské pohlaví $7 D008297
650    12
$a spermie $x metabolismus $7 D013094
650    12
$a testikulární nádory $x metabolismus $x patologie $7 D013736
650    12
$a kryoprezervace $x metody $7 D015925
650    12
$a energetický metabolismus $7 D004734
650    12
$a oxidativní fosforylace $7 D010085
650    12
$a glykolýza $7 D006019
650    12
$a germinální a embryonální nádory $x metabolismus $x patologie $7 D009373
650    _2
$a dospělí $7 D000328
650    _2
$a spotřeba kyslíku $x fyziologie $7 D010101
650    _2
$a mitochondrie $x metabolismus $7 D008928
650    _2
$a zachování plodnosti $x metody $7 D059247
650    _2
$a uchování spermatu $x metody $7 D012662
655    _2
$a časopisecké články $7 D016428
700    1_
$a Bryndova, Barbora $u Laboratory of Reproductive Biology, Institute of Biotechnology of the Czech Academy of Sciences, Vestec, Czech Republic $u Department of Biochemistry, Faculty of Science, Charles University, Prague, Czech Republic
700    1_
$a Sur, Vishma Pratap $u Laboratory of Reproductive Biology, Institute of Biotechnology of the Czech Academy of Sciences, Vestec, Czech Republic
700    1_
$a Ded, Lukas $u Laboratory of Reproductive Biology, Institute of Biotechnology of the Czech Academy of Sciences, Vestec, Czech Republic
700    1_
$a Cockova, Zuzana $u Imaging Methods Core Facility at BIOCEV, Faculty of Science, Charles University, Vestec, Czech Republic
700    1_
$a Benda, Ales $u Imaging Methods Core Facility at BIOCEV, Faculty of Science, Charles University, Vestec, Czech Republic
700    1_
$a Qasemi, Maryam $u Laboratory of Reproductive Biology, Institute of Biotechnology of the Czech Academy of Sciences, Vestec, Czech Republic
700    1_
$a Pecina, Petr $u Laboratory of Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, Czech Republic
700    1_
$a Pecinova, Alena $u Laboratory of Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, Czech Republic
700    1_
$a Spevakova, Daniela $u Laboratory of Reproductive Biology, Institute of Biotechnology of the Czech Academy of Sciences, Vestec, Czech Republic
700    1_
$a Hradec, Tomas $u Department of Urology, General University Hospital and First Faculty of Medicine, Charles University, Prague, Czech Republic
700    1_
$a Skrobanek, Pavel $u Department of Oncology, First Faculty of Medicine, Charles University and Thomayer University Hospital, Prague, Czech Republic
700    1_
$a Ezrova, Zuzana $u Laboratory of Molecular Therapy, Institute of Biotechnology of the Czech Academy of Sciences, BIOCEV, Vestec, Czech Republic
700    1_
$a Kratka, Zuzana $u Laboratory of Immunology, IVF Clinic GENNET, Prague, Czech Republic
700    1_
$a Kren, Radomir $u Laboratory of Embryology, IVF Clinic GENNET, Prague, Czech Republic
700    1_
$a Jeseta, Michal $u Department of Obstetrics and Gynaecology, Faculty of Medicine, Masaryk University and University Hospital Brno, Brno, Czech Republic
700    1_
$a Boublikova, Ludmila $u Department of Oncology, First Faculty of Medicine, Charles University and Thomayer University Hospital, Prague, Czech Republic
700    1_
$a Zamecnik, Libor $u Department of Urology, General University Hospital and First Faculty of Medicine, Charles University, Prague, Czech Republic
700    1_
$a Büchler, Tomas $u Department of Oncology, First Faculty of Medicine, Charles University and Thomayer University Hospital, Prague, Czech Republic $u Department of Oncology, Second Faculty of Medicine, Motol University Hospital, Prague, Czech Republic
700    1_
$a Neuzil, Jiri $u Laboratory of Molecular Therapy, Institute of Biotechnology of the Czech Academy of Sciences, BIOCEV, Vestec, Czech Republic $u School of Pharmacy and Medical Science, Griffith University, Southport, QLD, Australia
700    1_
$a Postlerova, Pavla $u Laboratory of Reproductive Biology, Institute of Biotechnology of the Czech Academy of Sciences, Vestec, Czech Republic
700    1_
$a Komrskova, Katerina $u Laboratory of Reproductive Biology, Institute of Biotechnology of the Czech Academy of Sciences, Vestec, Czech Republic $u Department of Zoology, Faculty of Science, Charles University, Prague, Czech Republic $1 https://orcid.org/0000000268372148 $7 xx0283824 jx20100128015
773    0_
$w MED00005120 $t Molecular human reproduction $x 1460-2407 $g Roč. 31, č. 1 (2025)
856    41
$u https://pubmed.ncbi.nlm.nih.gov/40053689 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y - $z 0
990    __
$a 20250415 $b ABA008
991    __
$a 20250429134840 $b ABA008
999    __
$a ok $b bmc $g 2311540 $s 1247314
BAS    __
$a 3
BAS    __
$a PreBMC-MEDLINE
BMC    __
$a 2025 $b 31 $c 1 $e 20250117 $i 1460-2407 $m Molecular human reproduction $n Mol Hum Reprod $x MED00005120
GRA    __
$a NU20-03-00309 $p Ministry of Health of the Czech Republic
GRA    __
$a GA22-21082S $p Czech Science Foundation
LZP    __
$a Pubmed-20250415

Najít záznam

Citační ukazatele

Možnosti archivace