Metastatic colorectal cancer is one of the most critical causes of cancer-related dead in patients suffering this type of malignancy. As the liver metastases are found in almost 25-50% of all colorectal cancer patients, there is an urgent need for suitable in vitro and in vivo models. To the best of our knowledge, only a limited number of well-described 2D or 3D organoid/spheroid cell lines originated from human liver metastases was reported for this type of malignancy and submitted to ATCC and other repository centers for general use. Here, we present a novel, well-characterized cell line Coala that was derived directly from liver metastasis of patient with colorectal cancer. Coala cancer cell line was derived by 2D and 3D culture technology and can be, therefore, cultured as standard 2D cell line or as 3D spheroid culture. When transplanted into athymic mice, Coala cell line forms fast growing tumors with histological features similar to original tumor and expressed CDX2, SATB2, and CK20. Whole exome sequencing analysis of Coala cell line showed the presence of pathogenic mutations in C8B, PRKCQ, IRGM, FGFR4, POLR1C, APC and TP53 genes. As verified by antibody array chip analysis, Coala cells express Snail, Pdx-1, FoxA2, and E-cadherin. Our cell line represents a new, well characterized in vitro research tool for cancer research and will be available to researchers in the field via biorepository centers.

Biomedical Centre Martin Jessenius Faculty of Medicine in Martin Comenius University in Bratislava Mala Hora 4C 036 01 Martin Slovakia

Biomedical Research Center of Slovak Academy of Sciences Cancer Research Institute Dubravska cesta 9 845 05 Bratislava Slovakia

Central European Institute of Technology Faculty of Medicine Masaryk University Brno Czech Republic

Clinic of Radiology Jessenius Faculty of Medicine in Martin and University Hospital Martin Comenius University in Bratislava 036 01 Martin Slovakia

Clinic of Surgery and Transplant Center Jessenius Faculty of Medicine in Martin and University Hospital Martin Comenius University in Bratislava 036 01 Martin Slovakia

Department of Clinical Genetics Jessenius Faculty of Medicine in Martin and University Hospital Martin Comenius University in Bratislava 036 01 Martin Slovakia

Department of Genetics The National Institute of Oncology Bratislava Slovakia

Department of Internal Medicine Hematology and Oncology University Hospital Brno Brno Czech Republic

Department of Medical Biochemistry Jessenius Faculty of Medicine in Martin Comenius University in Bratislava 036 01 Martin Slovakia

Department of Molecular Biology and Genomics Jessenius Faculty of Medicine in Martin Comenius University in Bratislava 036 01 Martin Slovakia

Department of Pathological Anatomy Jessenius Faculty of Medicine in Martin and University Hospital Martin Comenius University in Bratislava 036 01 Martin Slovakia

Department of Pathological Physiology and Central Animal Facility Jessenius Faculty of Medicine in Martin Comenius University in Bratislava 036 01 Martin Slovakia

GAMMA ZA 911 01 Trencin Slovakia

Institute of Medical Biology Genetics and Clinical Genetics Faculty of Medicine Comenius University 811 08 Bratislava Slovakia

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