Effect of inhibitors on acid production by baker's yeast
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
105974
DOI
10.1007/bf02885568
Knihovny.cz E-zdroje
- MeSH
- arseničnany farmakologie MeSH
- azidy farmakologie MeSH
- dicyklohexylkarbodiimid farmakologie MeSH
- dinitrofenoly farmakologie MeSH
- dusičnan uranylu farmakologie MeSH
- EDTA farmakologie MeSH
- ethanol farmakologie MeSH
- ethylmaleimid farmakologie MeSH
- fluorid sodný farmakologie MeSH
- jodacetamid farmakologie MeSH
- kyanid draselný farmakologie MeSH
- kyseliny metabolismus MeSH
- nitrily farmakologie MeSH
- oligomyciny farmakologie MeSH
- pikráty farmakologie MeSH
- Saccharomyces cerevisiae metabolismus MeSH
- trinitrobenzeny farmakologie MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- arseničnany MeSH
- azidy MeSH
- dicyklohexylkarbodiimid MeSH
- dinitrofenoly MeSH
- dusičnan uranylu MeSH
- EDTA MeSH
- ethanol MeSH
- ethylmaleimid MeSH
- fluorid sodný MeSH
- jodacetamid MeSH
- kyanid draselný MeSH
- kyseliny MeSH
- nitrily MeSH
- oligomyciny MeSH
- pikráty MeSH
- trinitrobenzeny MeSH
Glucose-induced acid extrusion, respiration and anaerobic fermentation in baker's yeast was studied with the aid of sixteen inhibitors. Uranyl(2+) nitrate affected the acid extrusion more anaerobically than aerobically; the complexing of Mg2+ and Ca2+ by EDTA at the membrane had no effect. Inhibitors of glycolysis (iodoacetamide, N-ethylmaleimide, fluoride) suppressed acid production markedly, and so did the phosphorylation-blocking arsenate. Fluoroacetate, inhibiting the citric-acid cycle, had no effect. Inhibition by uncouplers depended on their pKa values: 2,4,6-trinitrophenol (pKa 0.4) less than 2,4-dinitrophenol (4.1) less than azide (4.7) less than 3-chlorophenylhydrazonomalononitrile (6.0). Inhibition by trinitrophenol was only slightly increased by its acetylation. Cyanide and nonpermeant oligomycin showed practically no effect; inhibition by dicyclohexylcarbodiimide was delayed but potent. The concentration profiles of inhibition of acid production differed from those of respiration and fermentation. Thus, though the acid production is a metabolically dependent process, it does not reflect the intensity of metabolism, except partly in the first half of glycolysis.
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Extrusion of metabolites from baker's yeast during glucose-induced acidification
