Synthesis and degradation of proteins and DNA in Streptomyces aureofaciens
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
112013
DOI
10.1007/bf02926450
Knihovny.cz E-zdroje
- MeSH
- alanintransaminasa metabolismus MeSH
- aspartátaminotransferasy metabolismus MeSH
- bakteriální proteiny metabolismus MeSH
- chloramfenikol farmakologie MeSH
- DNA bakterií metabolismus MeSH
- leucin metabolismus MeSH
- Streptomyces aureofaciens metabolismus MeSH
- tetracykliny farmakologie MeSH
- thymin metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- alanintransaminasa MeSH
- aspartátaminotransferasy MeSH
- bakteriální proteiny MeSH
- chloramfenikol MeSH
- DNA bakterií MeSH
- leucin MeSH
- tetracykliny MeSH
- thymin MeSH
The rate of protein synthesis in Streptomyces aureofaciens, measured by incorporation of U-14C-L-leucine into cells, fluctuated during the production phase in the range of 10-15% of the values determined in the phase of intensive growth. Tetracycline partially inhibited the protein synthesis during the growth phase only. The proteins synthesized between the 6th and 18th hour of growth, were 75% degraded by the 48th hour. The DNA synthesis, measured by means of incorporation of 2-14C-thymine into the mycelium, occurred predominantly during the first 24 h of cultivation. Similarly, DNA synthesized between the 6th and 12th hour of cultivation was degraded by 75% after 48 h. The turnover of culture proteins is thus caused largely by degradation of old cells and growth of new ones which are more resistant to tetracycline. The activity of alanine aminotransferase and aspartate aminotransferase increase substantially towards the end of fermentation.
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