Cytochemical and biological properties of Mycobacterium bovis BCG
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
330364
DOI
10.1007/bf02877655
Knihovny.cz E-zdroje
- MeSH
- antituberkulotika farmakologie MeSH
- azidy farmakologie MeSH
- BCG vakcína * MeSH
- dítě MeSH
- králíci MeSH
- kultivační média MeSH
- lidé MeSH
- morčata MeSH
- Mycobacterium bovis účinky léků enzymologie MeSH
- nikotinamidasa metabolismus MeSH
- předškolní dítě MeSH
- ureasa metabolismus MeSH
- zvířata MeSH
- Check Tag
- dítě MeSH
- králíci MeSH
- lidé MeSH
- morčata MeSH
- mužské pohlaví MeSH
- předškolní dítě MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antituberkulotika MeSH
- azidy MeSH
- BCG vakcína * MeSH
- kultivační média MeSH
- nikotinamidasa MeSH
- ureasa MeSH
It was the aim of the present communication to find a simple test for a reliable discrimination of Mycobacterium bovis BCG from Mycobacterium tuberculosis. A total of 26 BCG strains, out of them 10 Czechoslovak strains (2 lyophilized cultures of BCG of different batch, 6 strains isolated from abscesses of children after BCG-vaccination and 2 strains from fatal cases after BCG-vaccination) and 16 strains obtained from foreign laboratories, were used. Of the tested characteristics a combination of 3 tests, sensitivity to 1 microgram of 2-thiophene carbonylhydrazide (TCH), activity of 3 acylamidases (urease, nicotinamidase and pyrazinamidase) and a quantitative nitrate test, was found to be most advantageous. The Czechoslovak strains of Mycobacterium bovis BCG were fully sensitive to TCH, of the 3 acylamidases mentioned above only urease was positive and nitrate was reduced only little or not at all. On the other hand, strains of Mycobacterium tuberculosis were always resistant to TCH, had positive urease, nicotinamidase and pyrazinamidase and reduced nitrate very intensively.
