Degradation by Eco R1 endonuclease of DNA isolated from phages infecting Bacillus licheniformis and Bacillus thuringiensis
Language English Country United States Media print
Document type Journal Article
PubMed
648976
DOI
10.1007/bf02915314
Knihovny.cz E-resources
- MeSH
- Bacillus thuringiensis MeSH
- Bacillus MeSH
- Bacteriophages analysis metabolism MeSH
- DNA, Viral isolation & purification metabolism MeSH
- DNA Restriction Enzymes metabolism MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- DNA, Viral MeSH
- DNA Restriction Enzymes MeSH
DNA was isolated from lytic phages of two strains, Bacillus licheniformis, a producer of bacitracin, and Bacillus thuringiensis forming protein paracrystals with pronounced insecticidal effects. Its sensitivity to Eco R1 restriction endonuclease was determined. It was the aim of the work to find out whether these phages could serve as vectors in the transfer and possible amplification of genes of the two important industrial species of bacilli. Approximate values of the molecular weight of DNA of the two phages were determined after degradation of the phage DNA by Eco R1, followed by comparison of electrophoretic mobility of individual fragments with that of the Eco R1-degraded DNA of phage lambdab2.
